1. Art and Diseño

Page:
1 of 2
Customer No.:
2500
Date:
28.01.2015
Test Report No. 1
Test Order: GMO analysis - 5er screening + plant-IDs – qualitative
Receipt of sample: 19.01.2015, begin of analysis: 19.01.2015, end of analysis: 20.01.2015
Sample description: Bees protein feed of FeedBee, Apipowder and water, sample code:
without, quantity: approx. 1130g (including packing)
5er-screening (35S, NOS, epsps-, pat-, bar-Gen) + plant IDs
Sampling was carried out by the customer who is responsible for representativeness and logistics.
Packing of the sample: The sample was intact; there were no signs of manipulation.
Result
Screening
Plant IDs
Reference genes
35S-promoter
NOS-terminator
epsps-gene
pat-gene
bar-gene
Maize
Soy
Canola
Mustard
detected
detected
detected
detected
not detected
genetic patterns confirmed
genetic patterns confirmed
genetic patterns not confirmed
genetic patterns confirmed
universal reference gene
detected
In the sample the detection of the sequence corresponding to 35S-promoter is positive, to NOSterminator is positive, to epsps-gene is positive, to pat-gene is positive and to bar-gene is negative.
The detection of the universal reference gene demonstrates the presence of DNA in the sample which can
be amplified.
Responsible for the content: Dr. Rösel
Illertissen, ............................... .................................................................
Dr. S. Rösel, Dr. K. Neumann: laboratory director; Dr. E. Nüßeler: laboratory vice-director; J. Kühnemann: senior specialist
GMO analyses – detection of genetically modified DNA
Using Real Time PCR (polymerase chain reaction) DNA sequences of the genetic material which are
frequently used in genetically modified plants can be detected. In order to prove the informational value of
the PCR, an additional reference gene is analysed. This is a gene that is also found in not genetically
modified plants.
Method of analysis:
Homogenization of solid samples: The amount shown in the sample description is milled and
homogenized. 200 mg up to 2500 mg of the material are analysed.
Analysis was done as triple extraction.
DNA extraction method: The DNA is extracted using standard molecular biological methods (according to
DIN EN ISO 21571:2005).
Qualitative determination of 35S, NOS (LP-P-01-41 2014-09), epsps- (LP-P-01-50 2011-04), pat(LP-P-01-51 2011-04) and bar-gene (LP-P-01-50 2011-04): The analysis is done according to ASU L
00.00 122 and on the basis of the international valid standards DIN EN ISO 24276:2006 as well as DIN EN
ISO 21570:2005 using Real Time PCR (45 cycles), and different publicly available publications. The
sequence of 35S-promotor originates from the Cauliflower Mosaic Virus (CaMV) and can be found for
®
example in Roundup Ready -Soy and in several GM maize and canola events. This test does not
differentiate if the 35S promoter originates from one or more GMO plants or rather from the CaMV
(cauliflower mosaic virus). The sequence of the NOS-terminator (Nopalin Synthase Terminator) originates
from Agrobacterium tumefaciens, and is found amongst others in Roundup Ready®-Soy as well as in
several GM maize and canola events. epsps-gene: imparts tolerance towards the herbicide Roundup
®
Ready (as in the GM-canola event GT73 or in the maize event MON88017), detection of CTP2CP4epsps-construct. pat-gene: imparts a tolerance towards the herbicide BASTA, doing so amongst
others in GM-canola event Topas 19/2, Falcon GS4090 or maize event T25, detection of pCaMV35S-patconstruct. bar-gene: imparts a tolerance towards the herbicide BASTA, e.g. in the GM-canola events MS8
and RF3 or the GM-maize event Bt176.
Controls and reference material: In each analysis positive and negative controls were used. The
negative control does not contain DNA and serves to demonstrate the lack of contaminations.
Limit of detection (LOD): < 0,01% in raw materials. The limit of detection also depends on the kind of
sample matrix.
Interpretation: Qualitative Real Time-PCR (LP-P-02-07 2004-10)
The results of analyses done by lifeprint GmbH apply exclusively to the actually analyzed part of sample sent by the customer. They do not have to be
representative for the whole product / good, from which the sample was taken out by the customer. The accreditation is exclusively valid for the test
methods mentioned in the annex of the certificate of accreditation. Lifeprint GmbH excludes any liability for direct or indirect damages in respect to the
performed analysis. None of the test reports might be replicated, not even in parts or any other form (by copies, micro films etc.) or distributed externally by
using electronic systems. The test report is for the exclusive use of the customer. In addition our General Terms and Conditions are apply.
Page 2 of 2
Page:
1 of 2
Customer No.:
2500
Date:
28.01.2015
Test Report No. 2
Test Order: GMO analysis - plant-IDs – qualitative
Further test to test report 200840992
Receipt of sample: 19.01.2015, begin of analysis: 19.01.2015, end of analysis: 21.01.2015
Sample description: Bees protein feed of FeedBee, Apipowder and water, sample code:
without, quantity: aprox. 1130g (including packing)
Plant IDs
Sampling was carried out by the customer who is responsible for representativeness and logistics.
Packing of the sample: The sample was intact; there were no signs of manipulation.
Result
Plant IDs
Wheat
Alfalfa (Lucerne)
Rice
Potato
Brassicaceen
Sugar beet
Cotton
genetic pattern confirmed *
genetic pattern confirmed *
genetic pattern confirmed *
genetic pattern confirmed *
genetic pattern confirmed *
not detected
not detected
Reference genes
universal reference gene
detected
In the sample the detection of the genetic patterns for above mentioned parameters excluding sugar beet
and cotton were confirmed. The detection of the universal reference gene demonstrates the presence of
DNA in the sample which can be amplified.
*Evaluation: The raw data during the identification point to findings of traces.
Responsible for the content: Dr. Rösel
Illertissen, ............................... .................................................................
Dr. S. Rösel, Dr. K. Neumann: laboratory director; Dr. E. Nüßeler: laboratory vice-director; J. Kühnemann: senior specialist
GMO analyses – detection of genetically modified DNA
Using Real Time PCR (polymerase chain reaction) DNA sequences of the genetic material which are
frequently used in genetically modified plants can be detected. In order to prove the informational value of
the PCR, an additional reference gene is analysed. This is a gene that is also found in not genetically
modified plants.
Method of analysis:
Homogenization of solid samples: The amount shown in the sample description is milled and
homogenized. 200 mg up to 2500 mg of the material are analysed.
All analyses are done as double extraction.
DNA extraction method: The DNA is extracted using standard molecular biological methods (according to
DIN EN ISO 21571:2005).
Qualitative determination of cotton (LP-P-04-06 2007-12); wheat (LP-P-04-37 2014-02); Alfalfa (LP-P04-34 2013-11); rice (LP-P-04-07 2006-09) and sugar beet (LP-P-04-08 2004-08), potato (LP-P-04-05
2008-02) and Brassicaceen (LP-P-04-03 2002-03): The analysis is done according to
ASU L 00.00 122 and on the basis of the international valid standards DIN EN ISO 24276:2006 as well as
DIN EN ISO 21570:2005 using Real Time PCR (45 cycles), and different publicly available publications.
The detection of the respective species-specific genes shows the presence of this plant. A statement if the
sample contains genetically modified components is not done by this analysis.
Controls and reference material: In each analysis positive and negative controls were used. The
negative control does not contain DNA and serves to demonstrate the lack of contaminations.
Limit of detection (LOD): < 0,01% in raw materials. The limit of detection also depends on the kind of
sample matrix.
Interpretation: Qualitative Real Time-PCR (LP-P-02-07 2004-10)
The results of analyses done by lifeprint GmbH apply exclusively to the actually analyzed part of sample sent by the customer. They do not have to be
representative for the whole product / good, from which the sample was taken out by the customer. The accreditation is exclusively valid for the test
methods mentioned in the annex of the certificate of accreditation. Lifeprint GmbH excludes any liability for direct or indirect damages in respect to the
performed analysis. None of the test reports might be replicated, not even in parts or any other form (by copies, micro films etc.) or distributed externally by
using electronic systems. The test report is for the exclusive use of the customer. In addition our General Terms and Conditions are apply.
Page 2 of 2
Page:
Customer No.:
Date:
1 of 2
2500
28.01.2015
Test Report No. 3
Test Order: GMO analysis – soy variety IDs – qualitative
Further test to test report no. 200840992
Receipt of sample: 19.01.2015, begin of analysis: 19.01.2015, end of analysis: 21.01.2015
Sample description: Bees protein feed of FeedBee, Apipowder and water, sample code: without,
quantity: aprox. 1130g (including packing)
Soy variety IDs
Sampling was carried out by the customer who is responsible for representativeness and logistics.
Packing of the sample: The sample was intact, there were no signs of manipulation.
Result
Soy
GTS-40-3-2 (RRS-1)
detected
detected
not detected
not detected
not detected
not detected
not detected
not detected
not detected
Reference gene
MON89788 (RRS-2)
FG72
CV127
305423
MON87705
MON87708
A2704
A5547
soy specific reference gene
detected *
In the sample the detection of the sequence corresponding to GTS-40-3-2 and MON89788 is positive.
The detection of the soy specific reference gene demonstrates the presence of DNA in the sample which
can be amplified.
* Evaluation: The sample contains relatively few soy specific DNA sequences. This indicates
comparatively low soy contents.
Responsible for the content: Dr. Rösel
Illertissen,...............................
.................................................................
Dr. S. Rösel, Dr. K. Neumann: laboratory director; Dr. E. Nüßeler: laboratory vice-director; J. Kühnemann: senor specialist
GMO analyses – detection of genetically modified DNA
Using Real Time PCR (polymerase chain reaction) DNA sequences of the genetic material which are
frequently used in genetically modified plants can be detected. In order to prove the informational value of
the PCR, an additional reference gene is analysed. This is a gene that is also found in not genetically
modified plants.
Method of analysis:
Homogenization of solid samples: The amount shown in the sample description is milled and
homogenized. 200 mg up to 2500 mg of the material are analysed.
All analyses are done as double extraction.
DNA extraction method: The DNA is extracted using standard molecular biological methods (according to
DIN EN ISO 21571:2005).
Qualitative determination of the GMO soy variety GTS-40-3-2 (RRS-1, MON-Ø4Ø32-6) (LP-P-01-08
2001-05), MON89788 (RRS-2, MON-89788-1) (LP-P-01-09 2008-08), MON87705 (MON877Ø5-6) (LP-P01-65 2013-08), MON87708 (MON-877Ø8-9) (LP-P-01-70 2014-05), FG72 (MST-FGØ72-2) (LP-P-01-68
2013-09), CV127 (BPS-CV127-9) (LP-P-01-61 2013-07), 305423 (DP-3Ø5423-1) (LP-P-01-39 2009-10),
A2704-12 (ACS-GMØØ5-3) (LP-P-01-10 2008-07) and A5547-127 (ACS-GMØØ6-4) (LP-P-01-47): The
analysis is done by Real Time-PCR (45 cycles) according to protocols of the Joint Research Centre (JRC)
of the European Commission, on the basis of the international valid standards as well as different publicly
available publications.
Controls and reference material: In each analysis positive and negative controls were used. The
negative control does not contain DNA and serves to demonstrate the lack of contaminations.
Limit of Detection (LOD):
Roundup Ready-Soy-1: < 0,01% in raw materials; MON89788: ≤ 0,045% in raw materials; A2704-12;
FG72; A5547: ≤ 0,023 % in raw materials; CV127; 305423; MON87705; MON87708: < 0,04%. The limit of
detection also depends on the kind of sample matrix.
Interpretation: Qualitative Real Time-PCR (LP-P-02-07 2004-10)
The results of analyses done by lifeprint GmbH apply exclusively to the actually analyzed part of sample sent by the customer. They do not have
to be representative for the whole product / good, from which the sample was taken out by the customer. The accreditation is exclusively valid for
the test methods mentioned in the annex of the certificate of accreditation. Lifeprint GmbH excludes any liability for direct or indirect damages in respect to
the performed analysis. None of the test reports might be replicated, not even in parts or any other form (by copies, micro films etc.) or distributed
externally by using electronic systems. The test report is for the exclusive use of the customer. In addition our General Terms and Conditions apply.
Page 2 of 2
Page:
Customer No..:
Date:
1 of 3
2500
28.01.2015
Test Report No. 4
Test Order: GMO analysis – maize variety IDs – qualitative
Further test to test report no. 200840992
Receipt of sample: 19.01.2015, begin of analysis: 19.01.2015, end of analysis: 21.01.2015
Sample description: Bees protein feed of FeedBee, Apipowder and water, sample code: without,
quantity: aprox. 1130g (including packing)
Maize variety IDs
Sampling was carried out by the customer who is responsible for representativeness and logistics.
Packing of the sample: The sample was intact, there were no signs of manipulation.
Result
Maize
Reference gene
MON89034
detected
NK603
detected
DAS59122
detected
MIR604
detected
Bt11
detected
TC1507
detected
MIR162
detected
MON88017
detected
MON810
detected
GA21
Bt176
3272
98140
T25
DAS40278
LY038
MON863
MON87460
detected
not detected
not detected
not detected
not detected
not detected
not detected
not detected
not detected
maize specific reference gene
detected
In the sample the detection of the sequence corresponding to MON89034, NK603, DAS59122, MIR604,
Bt11, TC1507, MIR162, MON88017, MON810 and GA21 is positive, for Bt176, 3272, 98140, T25,
DAS40278, LY038, MON863 and MON87460 it is negative.
The detection of the maize specific reference gene demonstrates the presence of DNA in the sample which
can be amplified.
Responsible for the content: Dr. Rösel
Illertissen,...............................
.................................................................
Dr. S. Rösel, Dr. K. Neumann: laboratory director; Dr. E. Nüßeler: laboratory vice-director; J. Kühnemann: senior specialist
The results of analyses done by lifeprint GmbH apply exclusively to the actually analyzed part of sample sent by the customer. They do not have to be
representative for the whole product / good, from which the sample was taken out by the customer. The accreditation is exclusively valid for the test
methods mentioned in the annex of the certificate of accreditation. Lifeprint GmbH excludes any liability for direct or indirect damages in respect to the
performed analysis. None of the test reports might be replicated, not even in parts or any other form (by copies, micro films etc.) or distributed externally by
using electronic systems. The test report is for the exclusive use of the customer. In addition our General Terms and Conditions are apply.
Page 2 of 3
GMO analyses – detection of genetically modified DNA
Using Real Time PCR (polymerase chain reaction) DNA sequences of the genetic material which are
frequently used in genetically modified plants can be detected. In order to prove the informational value of
the PCR, an additional reference gene is analysed. This is a gene that is also found in not genetically
modified plants.
Method of analysis:
Homogenization of solid samples: The amount shown in the sample description is milled and
homogenized. 200 mg up to 2500 mg of the material are analysed.
All analyses are done as double extraction.
DNA extraction method: The DNA is extracted using standard molecular biological methods (according to
DIN EN ISO 21571:2005).
Qualitative determination of GMO maize varieties MON88017 (MON88Ø17-3) (LP-P-01-40 2009-07),
NK603 (MON-ØØ6Ø3) (LP-P-01-19 2007-03), MON810 (MON-ØØ81Ø-6) (LP-P-01-14 2004-10), TC1507
(DAS-Ø15Ø7-1) (LP-P-01-18 2005-10), DAS59122 (DAS-59122-7) (LP-P-01-16 2007-11); T25 (ACSZMØØ3-2) (LP-P-01-15 2007-11), Bt11 (SYN-BTØ11-1) (LP-P-01-13 2006-03), Bt176 (SYN-EV176-9)
(LP-P-01-17 2002-01), MON89034 (MON-89Ø34-3) (LP-P-01-33 2010-02), 3272 (SYN-E3272-5) (LP-P-0145 2011-04), 98140 (DP-Ø9814Ø-6) (LP-P-01-46 2011-04), DAS40278-9 (DAS-4 278-9) (LP-P-01-74
2014-10); MIR604 (SYN-IR6 4-5) (LP-P-01-12 2007-05); LY038 (RENMIR162 (SYN-IR162-4) (LP-P-01-53 2011-07); MON863 (MONMON87460 (MON-8746 -4) (LP-P-01-66 2013-09); GA21 (MON-
38-3) (LP-P-01-73 2014-10);
863-5) (LP-P-01-11 2001-05);
21-9) (LP-P-01-20 2005-10): The
analysis was done by Real Time-PCR (45 cycles) according to protocols of the Joint Research
Centre (JRC) of the European Commission, on the basis of the international valid standards as well as
different publicly available publications.
Controls and reference material: In each analysis positive and negative controls were used. The
negative control does not contain DNA and serves to demonstrate the lack of contaminations.
Limit of Detection (LOD):
Bt176: < 0,01% in raw materials; Bt11; MON89034; 3272; MON98140; MIR162; MON87460: 0,04% in raw
materials; DAS40278: <0,04 % in raw materials; MON88017, TC1507, DAS59122, MON810; T25; MIR604;
LY038: ≤ 0,045% in raw materials; NK603; MON863; GA21: ≤ 0,05% in raw materials. The limit of
detection also depends on the kind of sample matrix.
Interpretation: Qualitative Real Time-PCR (LP-P-02-07 2004-10)
The results of analyses done by lifeprint GmbH apply exclusively to the actually analyzed part of sample sent by the customer. They do not have to be
representative for the whole product / good, from which the sample was taken out by the customer. The accreditation is exclusively valid for the test
methods mentioned in the annex of the certificate of accreditation. Lifeprint GmbH excludes any liability for direct or indirect damages in respect to the
performed analysis. None of the test reports might be replicated, not even in parts or any other form (by copies, micro films etc.) or distributed externally by
using electronic systems. The test report is for the exclusive use of the customer. In addition our General Terms and Conditions are apply.
Page 3 of 3