From www.bloodjournal.org by guest on February 6, 2015. For personal use only. Prognostic Factors in Agnogenic Myeloid Metaplasia: A Report on 195 Cases With a New Scoring System By Brigitte Dupriez, Pierre Morel, Jean L. Demon/, Jean L. Lai, Marc Simon, Isabelle Plantier, and Francis Bauters We studied the survival of 195 patients with agnogenic myeloid metaplasia (AMM) diagnosed between 1962 and 1992 in an attempt to stratify patients into risk groups. Median survival was 42 months. Adverse prognosticfactors for survival were age > 60 years, hepatomegaly, weight loss, low hemoglobin level (Hb), low or very high leukocyte count (WBC), high percentage of circulating blasts, male sex, and low platelet count. A new scoring system based on two adverse prognostic factors, namely Hb e 10 g/dL and WBC e 4 or >30 x l@/L, was able to separate patients in three groups with low (0 factor), intermediate (1 factor), and high (2 factors) risks, associated with a median survival of 93, 26, and 13 months, respectively.An abnormal karyotype (32 cases of 94 tested patients) was associated with a short survival, especially in the low-risk group (median survival of 50 v 112 months in patients with normal karyotype). The prognostic factors for acute conversion were WBC > 30 x 10s/L and abnormal karyotype. Thus, hemoglobin level and leukocyte count provide a simple prognostic model for survival in AMM, and the adverse prognosticvalue of abnormal karyotype may be related to a higher rate of acute conversion. 0 1996 by The American Society of Hematology. A single agent oral chemotherapy (hydroxyurea, 78 cases; busulfan, 12 cases; pipobroman, 10 cases); 27 patients with cytopenias received androgen; 25 patients underwent splenectomy, usually when other treatments failed; and 3 elderly patients had splenic irradiation because of surgical contraindication. Prognostic factors analyzed. The following initial characteristics were analyzed in all patients: age, sex, spleen size, hepatomegaly, weight loss, hemoglobin concentration (Hb), white blood cell count (WBC), platelet count, percentage of circulating blasts, percentage of immature granulocytes (IG, excluding blasts), reticulocyte count, and MF staging at BM biopsy. Njoku and Visani scoring Njoku et a15identified four classes of decreassystems were ing survival from I to IV, by using Hb level (classes 1 to 11, >10 g/dL; classes I11 and IV, < 10 g/dL) and reticulocyte count (classes I and IV, <2 %; classes I1 to 111, >2%). Visani et a12 identified three prognostic subgroups by taking the following adverse prognostic factors into account: Hb < 10 g/dL and percentage of WBC precursors (blasts, promyelocytes, and myelocytes) > 10%. Low-risk patients had no adverse prognostic factor, intermediate-risk patients only had anemia, and high-risk patients had WBC precursors > lo%, independent of Hb level. Statistical analysis. All analyses were performed with the statistical application system (SAS) software (SAS Institute Inc, Cary, NC). The closing date was June 1, 1994. The survival curves were drawn by the Kaplan-Meier product limit method.” Survival was measured from diagnosis to death or last follow-up. Progression to ANLL was defined by the presence of >30% BM blasts or >20% PB blasts. Statistical comparisons between actuarial survival curves were based on log rank and Cox tests. The parameters significant at the .05 level for survival were introduced in a first proportional hazards model regression analysis of survival (Cox model) with the forward stepwise ~election.’~ The GNOGENIC MYELOID metaplasia (AMM)is a myeloproliferative disorder characterized by bone marrow (BM) fibrosis and extramedullary hematopoiesis. Individual clinical course ranging from 1 to over 30 years has been reported, with a median survival of approximately 4 years from diagnosis.’-’ Progressive marrow failure, transformation into acute nonlymphoblastic leukemia (ANLL), and portal hypertension (PHT) are the major causes of death. Several clinical and biological prognostic parameters have been studied, with varying and sometimes conflicting results, except for anemia, constantly associated with short survival.’-7In addition, we previously reported the adverse prognostic value of abnormal karyotype.6 In this study on 195 AMM patients, we report a prognostic analysis of survival and progression to ANLL and PHT.Using multivariate analysis, we develop a new simple scoring system for clinical use and confirm the independent prognostic value of cytogenetics. MATERIALS AND METHODS Patients. Between January 1962 and December 1992, we diagnosed AMM in 195 patients. All patients fulfilled the polycythemia vera study group criteria for A M M ,which were splenomegaly, red cell poikilocytosis, leukoerythroblastosis, absence of monocytosis, BM fibrosis without any identifiable cause, and absence of Philadelphia chromosome.’ Patients with postpolycythemia myelofibrosis (MF), acute (malignant) MF, and myelodysplastic syndromes with MF were excluded? Hematologic examinations were performed by standard methods: peripheral blood (PB) and BM May-Griinwald-Giemsastained films. BM trephine biopsy was performed at diagnosis in all patients and stained with standard and silver stain for reticulin fibers; three stages of MF were defined according to the usual classification.” Cytogenetic analysis was available at diagnosis in 94 of 133 patients diagnosed since 1980 (48.2% of the whole group). Metaphases were obtained on BM or blood samples, after short-term (24 hours) culture without stimulation. Chromosomes were analyzed with conventional Giemsa stain and the R banding technique and classified according to the Intemational System for Cytogenetic Nomenclature.” Any anomaly present in at least three cells was considered clonal. Treatment. Treatment was based on age, presence of a poor performance status, importance of cytopenias, or myeloproliferative features. More precisely, 34 asymptomatic patients received no treatment and 52 received supportive care only (essentially transfusion). One hundred nine patients received at least one of the following therapies: 82 patients with very large or symptomatic splenomegaly and very high white cell or platelet counts received one or more 81004VOI 88, NO 3 (August 1). 1996: pp 1013-1018 From the Service des Maladies du Sang, the Service de Cytogdndtique, Centre Hospitalier Rdgional et Universitaire de Lille; the Service d’Hdmatologie Clinique, Centre Hospitalier de Lens; the Laboratoire, Hdpital Saint Vincent, Lille; The Service d’Hdmtologie Clinique, Centre Hospitalier Gdndral de Valenciennes, France. Submitted November 27, 1995; accepted March 27, 19%. Address reprint requests to Brigitte Dupriez, MD, Service d ’ H d m tologie Clinique, Centre Hospitalier Dr Schafier, 62307 Lens Cedex, France. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C.section 1734 solely to indicate this fact. 0 1996 by The American Society of Hematology. 0006-497I/96/8803-0023$3.00/0 1013 From www.bloodjournal.org by guest on February 6, 2015. For personal use only. 1014 DUPRIEZ ET AL procedure was stopped when the P for entering an additional factor was above .05. Using the UNIFORM function of the SAS software, we randomly divided the 195 patients into a training and a test subsets containing 130 and 65 cases, respectively. Thus, we could develop a prognostic index in the training sample and test it in the other sample. We carried out a second proportional hazards model regression analysis of survival (Cox model) with the forward stepwise selection in the training sample. Initial characteristics selected by this multivariate analysis of survival were included in a third proportional hazards model regression analysis of survival performed in the training sample with the best selection. Thus, only two variables were selected for inclusion in a scoring system (named LILLE score). For routine clinical use, we examined a categorized model with the two selected variables and their optimal cutoff values. We validated this model in the test sample and in the overall group of 195 patients with the log-rank test. Because karyotype was available in only 94 patients, the prognostic value of this variable (normal v abnormal) was assessed separately by univariate analysis, and a last Cox model was built with the following covariates: karyotype and LILLE score. The prognostic factors for time to death from ANLL or PHT were analyzed by the log-rank test. SMR = OIE = I12/20.84 = 5.38 ( p < 10-4) Ob RESULTS Initial characteristics and clinical course. There were 105 males and 90 females, with a median age of 65 years (range 27 to 85). Sixty patients (30.8%) were 5 6 0 years old and 18 (9.2%) 5 5 0 years. Anemia was present in 139 cases (71.3%) and Hb < 10 gldL in 44.6%. WBC exceeded 30 X 109/L in 22 cases (11.2%), and 16 patients (8.2%) had leukopenia. Thrombocytopenia was encountered in 5 1 patients (26.2%) and thrombocytosis in 56 (28.7%). All but 7 patients (3.6%) had immature granulocytes, whereas 104 patients (53.3%) had PB blasts with a percentage 2 5% in only 19 cases (9.7%). Thirty-two patients (34%) had clonal chromosomal abnormalities, present either in all cells (19 cases) or coexisting with normal metaphases (13 cases with a mosaicism): de12Oq (11 cases); dell3q (8 cases); trisomy 8 (4cases); trisomy 21 or add (21) @11) (2 cases); de1(12)(q22) (2 cases); -Y (2 cases); and several others, accounting for one case each del(S)(pl4), -12, -17, -18, dup(l)(q32;q44), der(l7) t(12; 17)(q24;qll), 12, +19, t(13; 17)(q31;q22), t(1;7) (p31;p22), t(9;21)(p23;qll). Seven subjects exhibited complex aberrations. Patients with abnormal karyotype presented with weight loss more frequently than other patients (P = .02). No other difference in initial characteristics were found. With a median follow-up of 36 months, the median survival of the 195 patients was 42 months (range 1 to 228). At the time of the analysis, 148 patients (75.9%) had died, 40 were still alive 16+ to 216+ months from diagnosis, and 7 (3.6%) were lost to follow-up. The actuarial survival rates at 2 and 5 years were 68% 5 3% and 40% 2 4%, respectively (Fig 1). Thirty-three patients (22.3%) died from BM failure (severe anemia, infection, or hemorrhage) without ANLL transformation, 22 patients (14.9%) from ANLL transformation, 16 patients (10.8%) from PHT, 6 patients (4%) from cachexia, 18 patients (12.2%) from cardiovascular complications, 23 patients (15.5%) from unrelated causes, and 30 patients (20.3%) from unknown causes. Sixteen patients developed and died from PHT during the study period, 12 to 0 U 14 S Y M R M S Fig 1. Overall survival of 196 patients. The standard mortality ratio t.Chniqu.02' comp8md the mort.lity oburved before January 1, 1991 in the 180 p.tknts d i a g n d befora January 1, 1991, the date of the last reckoning In France, with the mortality exp.ot.d, during the Jmibr period of timo, in an age-and sex-matchod population of the Region Nod-Pas-de-Calais in Northern France. 159 months (median 84 months) after diagnosis of AMM. The actuarial cumulative risk of death from PHT was 7% at 5 years. Twenty-two patients developed and died from ANLL during the study period, 12 to 136 months (median 36 months) after diagnosis of AMM.The actuarial cumulative risks of death from leukemic transformation at 1 and 5 years were 2% and 16%, respectively. Treatment of both complications has only been symptomatic and palliative. Univariate anafysis of survival prognostic factors {Table 1). Hematologic parameters associated with adverse prognostic significance at the .01 level for both log-rank and Cox tests were, by decreasing order age > 60 years, hepatomegaly, weight loss, low Hb concentration, low or very high WBC count, high percentage of circulating blasts, male sex, low platelet count, and abnormal karyotype. The optimal cutoff value for Hb level was 10 g/a.Both patients with leukopenia or WBC counts > 30 X 109L had identical survival (Fig 2). Thus, this variable could be categorized into two subgroups, namely high-risk patients with WBC > 30 x 109L or <4 x 10% and low-risk patients with WBC 4 X lo9& and 5 3 0 X 109L. The percentage of immature granulocytes, the BM staging, the spleen size, and the reticulocyte count had no prognostic value for survival at the .01 level. From www.bloodjournal.org by guest on February 6, 2015. For personal use only. 1015 PROGNOSIS IN AGNOGENIC MYELOID METAPLASIA Table 1. Univariate Analysis Patients Median Survival (95% confidence interval) 66 129 80 (62-1 12) 36 (26-43) 10-4 90 105 64 (47-90) 36 (28-42) .001 121 74 48 (36-72) 38 (31-49) .I5 107 88 62 (48-90) 33 (24-37) 10-4 158 37 48 (42-73) 18 (12-33) 10-4 77 97 21 47 (38-59) 38 (30-72) 24 (15-111) .86 87 108 25 (18-36) 80 (54-96) 10-4 16 158 21 26 (13-48) 48 (38-73) 18 (12-36) 10-4 Fig 2. Survival of 195 patients according to WBC: (0).2 4 x 10'1 L and <30 x IV/L; (+I, <4 x l@/L; and (0I, >30 x lb/L. 51 144 26 (17-48) 48 (38-73) ,002 150 45 49 (42-78) 24 (17-36) 10-4 80 115 59 (43-82) 36 (24-48) .02 89 64 38 (26-54) 47 (31-79) .75 62 32 90 (54-112) 36 (27-44) .003 was available in only 94 patients, this variable was removed from the subsequent analysis. The Cox proportional hazards regression method, performed in the overall group of patients with all significant variables except karyotype, selected six parameters: Hb level, WBC count, weight loss, age, sex, and circulating blasts. They are listed in the order entered by the forward stepwise modeling procedure in Table 2. A similar forward stepwise regression analysis was performed in the training group of 130 patients. Six variables were selected: Hb level, WBC count, weight loss, sex, blasts, and age. Another regression analysis performed with the best selection in the training sample of patients identified an No. of Parameter Age (yr) s 60 >60 Sex Female Male Splenomegaly (cm below coastal margin) <IO 210 Hepatomegaly Absent Present Weight loss Absent Present BM staging I II 111 Hb (e/dL) <10 210 WBC (xlOa/dL) <4 4-30 >30 Platelets (xlOg/L) <150 ~150 Circulating blasts (%) s2 >2 Immature granulocytes (%) s5 >5 Reticulocytes (xlO*/L) SI00 >IO0 Karyotype Normal Abnormal P iili 1 ', 0 1) 14 l d l W R M S m Table 2. Multivariate Analysis (Stepwise Procedure) Therapeutic modalities did not influence survival, except for transfusion; survival of patients who required packed red cells was shorter than survival of patients who did not (P = .OOOl). This later correlation was probably related to the adverse prognostic value of anemia. As expected, the scoring systems of Njoku and Visani had a strong prognostic value for survival (P = However reticulocytes and WBC precursors (taking into account either IG alone or IG + blasts and cutoff value of 10% or 15%) retained no prognostic significance when the analyses were restricted to the subgroups of patients with a similar Hb level (< or 210 g/dL). Multivariate analysis of survival: Development and validation of the scoring system (Table 2). Because karyotype step Variable Entered Overall population (195 patients) 1 Hb WBC 2 Weight loss 3 Age 4 Sex 5 Blasts 6 Training set (130 points) 1 Hb WBC 2 3 Weight loss 4 Sex Blasts 5 6 Age Chi-square P 38.5 40.7 19.0 15.7 11.0 4.8 .0001 .0001 .0001 .0001 .009 .0282 25.9 24.2 13.3 15.1 6.7 6.3 .0001 .OOOl ,0003 .0001 .0094 .0116 From www.bloodjournal.org by guest on February 6, 2015. For personal use only. 1016 DUPRIEZ ET A1 Table 3. LlLLE Scoring System (For Predicting Survival in AMM) Adverse prognostic factors Hb <IO g/dL WBC <4 or >30 x 109/L The scoring system (number of adverse prognostic factors) Factor No. Risk Group Cases (96) Median Survival (mo) 0 Low Intermediate High 41 45 8 93 26 13 1 2 optimal model including two covariates: Hb level and WBC count. These two variables and their optimal cutoff values were included in a categorized Cox model. This model was nearly as effective (Wald chi-square 45.3) as the continuous model (Wald chi-square 52.9). We established a numerical scoring index with the same code system as the categorized regression model. A scoring system (Table 3)was constructed with the two major variables: Hb and WBC. One point each was allocated for Hb < 10 g/& and WBC < 4 X 109/L or >30 X 109/L. The total score (addition of the points for the two variables) separated the patients of the training group ( P = .OOOl), the test group ( P = .002), and the overall population into three subgroups. Low-risk patients (score 0; 47% of 195 patients), intermediate-risk patients (score l ; 45% of 195 patients), and high-risk patients (score 2; 8% of 195 patients) had a median survival of 93, 26, and 13 months, respectively (chi-square 110, P < (Fig 3). U- 04 0.i . 01- j 05 -: j 4 : 0.4 ; m f Uf S : 01: 01; 0.0 - 0 11 14 36 M 60 12 M I m Fig 3. Survival of 9 l5 patients according to the score: l+J, highrisk; (Dl, Intermediate risk; and IO ), low-risk. I 0.i . 01 M- 0 U 14 36 M I I Z U O I UONns Fig 4. Survival of patientsaccording to karyotype: (+), normel and (OJ,abnormal. Prognostic value of karyotype. An abnormal karyotype was also associated witha short survival (P = .003)in the 94 evaluable patients (Fig 4). Sixty of 94 patients with evaluable karyotype died: 36 of 62 with normal karyotype and 24 of 32 with an abnormal one. When the survival analysis was restricted to the karyotyped patients, the scoring system remained highly significant. Cytogenetics retained prognostic value in the low-risk group (48 cases); indeed, patients with normal karyotype (33 cases) had a mediansurvival of 112 months, whereas patients with abnormal karyotype (15 cases) had a median survival of 50 months ( P = .03) (Fig 5). Prognostic factors of death from ANU or PHT. Only two variables were found associated with a short time to death from ANLL: WBC count > 30 X 109/L( P = .OO01) and abnormal karyotype ( P = .Ol). Eleven deaths were related to acute conversion in the subgroup of patients with evaluable karyotype: 4 cases occurred in 36 patients with a normal karyotype (11%) and 7 cases in 32 patients (25%) with an abnormal one. There was no more leukemic conversion in the splenectomized patients than in other patients: 4 of 25 splenectomized patients died from ANLL (16%). Five variables were found associated with a short time to death from PHT:circulating blasts > 2% ( P = .001), weight loss ( P = .Ol), WBC count > 3O.lO9/L ( P = .02), hepatomegaly ( P = .03) and spleen z 10 cmunder the costal margin ( P = .M). DISCUSSION Despite of a large heterogeneity in median survival, several attempts have been made to identify clinical and labora- From www.bloodjournal.org by guest on February 6, 2015. For personal use only. 1017 PROGNOSIS IN AGNOGENIC MYELOID METAPLASIA and, more recently, cytogenetic studies and plasma soluble interleukin-2 receptors. On BM biopsy, decreased marrow cellularity and higher degree of fibrosis (III > I1 > I) was considered of adverse prognostic value," as was pattern of BM aplasia.' Patients with mild hepatic myeloid metaplasia M(HMM) survived longer than those with marked HMM in a clinicopathologic study of 22 cases.I4 01 Ferrokinetic studies provided sometimes conflicting results. A lower red cell volume was highly significant but not a larger plasma volume for Najean et al," and conversely for Njoku et a15; lower erythroid iron incorporation and higher hemolysis were also found ~ejorative'~; and a pattern of erythroid failure, described as class III by Barosi et al,1*16 usually correlated with BM aplasia, carried a bad prognostic witha high incidence of acute conversion. However, by multivariate analysis, only routine clinical and hematologic parameters retained prognostic value in our series as in others. Varki et a14 identified three risk groups on the basis of constitutional symptoms, platelet count, and Hb level. DJ Although most multivariate analyses agreed for the prognostic value of anemia, the other independent prognostic vari01ables remained controversial. Njoku et a15 proposed a score based on Hb and reticulocyte count. Barosi et a l l built a 0 U 11 X U ~ R M ~ prognostic classification tree in a series of 137 patients with age, Hb level, percentage of immature myeloid cells, and m histologic or isotopic BM features. Finally, Visani et a l' Fig 5. Survival of patients with low-risk score accordingto karyoidentified three prognostic subgroups by taking into account type: (+l, normal and (0). abnormal. percentage of WBC precursors and Hb concentration. These scores had strong prognostic value for survival; however, their prognostic value in the present series was probably only provided by Hb level. The prognostic index presented in tory features predicting survival. A short delay (<13 months) this report based only on two simple hematologic parameters between first signs and diagnosis,' the presence of constiturecorded at diagnosis (Hb level and WBC count) was able tional symptoms (fever, night sweats, andor weight loss),' to divide the patients into three groups withsignificantly spleen s i ~ e ? ' ~ *liver ' ~ size,' age > 60 years,244.10the different survival. In contrast with previous reports of progmale sex,'O anemia,'-5.'0"4thrombo~ytopenia,4.'~ reticulocyte nostic systems, we could validate the present scoring system count? increased percentage of immature granulocytes, a n d in a test population. or of circulating have all been found to influence The difference between our results and those of other survival. In the present study on 195 patients, until now the published series might be due to the following reasons: the largest study in A " , the clinical and hematologic features varying criteria for diagnosis (including or not postpolycyare similar to those reported in other large series. Univariate themic MF, acute MF, or myelodysplasia with MF), the analysis confirmed the prognostic value of most previously large heterogeneity of features and outcome in A " , and reported factors, especially anemia.'".'0.'4 Thrombocytopenia the large number of subjects required for the Cox propormoderately affected survival and was not selected in multitional hazard model. variate analysis, probably because of a correlation with aneWe previously reported the adverse prognostic value of mia, as previously demonstrated: Reticulocyte count, spleen abnormal karyotype: first suggested, albeit indirectly, in size, and BM staging had no prognostic value. To our knowl1982 by Besa et all8 and recently confirmed by Reilly et al.I9 edge, this is the first time that the leukocyte count at diagnoThe adverse prognostic value associated with an abnormal sis has been shown to be highly significant, for survival, karyotype among our low-risk patients suggested that the both in univariate and in multivariate analysis; both leukopepresent scoring system might be improved by cytogenetics. nia and high leukocytosis (>30 X 109/L)were associated The insufficient number of patients with available karyotype with short survival. The percentage of circulating blasts was at the time of the analysis precluded the selection of this also significant, but at a lower degree, in multivariate analyparameter in multivariate analysis. sis, and the percentage of immature granulocytes had only a moderate significant effect. Previous studies found progFew series reported on the prognostic factors for progresnostic value of immature granulocytes andor blasts,'.3 but sion to AML. High percentage of WBC precursors was assocomparison is difficult as their definition of WBC precursors ciated with frequent progression to AML and short survival included blasts and metamyelocytes. in twp previous studies." We found that high WBC count Other prognostic factors have been des~ribed,'.~,'~.'~"~ m' was associated with more frequent death from acute convercluding BM biopsy, liver biopsy, ferrokinetic parameters, sion, but high WBC precursors count was not. Patients with 7 From www.bloodjournal.org by guest on February 6, 2015. For personal use only. 1018 DUPRIEZ ET AL abnormal karyotype also carried a high risk of progression to AML, as suggested in our previous report^.^.^ These results are partially different from those of Reilly et al,I9 who only demonstrated a shorter survival for patients with abnormal karyotype and not increased risk of ANLL. Finally, an unexpected high rate of blastic transformation was observed in a recent study on 7 1 splenectomized patients, without significantly different survival.2o In conclusion, our study shows that the combination of Hb level and leukocyte count provides a useful and simple prognostic model for survival in AMM. In addition, we confirm the adverse prognostic value of abnormal karyotype, probably related in part to an increased risk of acute conversion. Treatment of A“ is still controversial. Our staging system could be useful to identify patients with limited life expectancy, for whom new therapeutic approaches might be investigated, mainly BM transplantation in younger ones. REFERENCES 1. Barosi G, Berzuini C, Liberato LN, Costa A, Polino G, Ascari E: A prognostic classification of myelofibrosis with myeloid metaplasia. Br J Haematol 70:397, 1988 2. Thiele J, Kvasnicka HM, Steinberg T, Zankovich R, Fischer R, Diehl V: Survival in primary (idiopathic) osteomyelofibrosis, socalled agnogenic myeloid metaplasia. Leuk Lymph 6:389, 1992 3. Visani G, Finelli C, Castelli U, Petti MC, Ricci N. Vianelli N, Gianni L, Zuffa E, Aloe Spiriti MA, Latagliata R, Pileri S, Magrini U, Gugliota L, Mona E, Bernasconi C, Mandelli F, Tura S: Myelofibrosis with myeloid metaplasia: Clinical and haematological parameters predicting survival in a series of 133 patients. Br J Haematol 75:4, 1990 4. Varki A, Lottenberg R, Griffith R, Reinhard E: The syndrome of idiopathic myelofibrosis. A clinico-pathologic review with emphasis on the prognostic variables predicting survival. Medicine 62:353, 1983 5. Njoku OS, Lewis SM, Catovski D, Gordon-Smith EC: Anaemia in myelofibrosis: Its value in prognosis. Br J Haematol 54:79, 1983 6. Demory JL, Dupriez B, Fenaux P, Lai’ JL, Beuscart R, Jouet JP, Deminatti M, Bauters F: Cytogenetic studies and their prognostic significance in agnogenic myeloid metaplasia: A report on 47 cases. Blood 72:855, 1988 7. Dupriez B, Demory JL, Lai JL, Fenaux P, Bauters F, Barosi G: Prognostic classification of myelofibrosis with myeloid metaplasia. Br J Haematol 73:136, 1989 (letter) 8. Laszlo J: Myeloproliferative disorders (MPD): Myelofibrosis, myelosclerosis, extramedullary hematopoiesis, undifferentiated MPD and hemorrhagic thrombocytemia. Semin Hematol4:409, 1975 9. Bearman RM, Pangalis GA, Rappaport H: Acute (“malignant”) myelosclerosis. Cancer 43:279, 1979 10. Chelloul N, BriBreJ, Laval-Jeantet M, Najean Y, Vorhauer W, Jacquillat C: Prognostic of myeloid metaplasia with myelofibrosis. Biomedicine 24:272, 1976 11. ISCN: An international system for human cytogenetic nomenclature. Cytogenet Cell Genet 21:309, 1978 12. Kaplan EL, Meier P: Nonparametric estimation from incomplete observations. J Am Stat Assoc 53:457, 1958 13. Cox DR: Regression models and lifetables (with discussion). JR Stat SOC34:187, 1972 14. Pereira A, Bruggera M, Cervantes F,Rozman C: Liver involvement at diagnosis of primary myelofibrosis. A clinicopathological study of twenty-two cases. Eur J Haematol 40:355, 1988 15. Najean Y, Cacchione R, Castro Malaspina H, Dresch C: Erythrokinetic studies in myelofibrosis: Their significance for prognosis. Br J Haematol 40205, 1978 16. Barosi G, Cazzola M, Frassoni F, Orlandi E, Stefanelli M: Erythropoiesis in myelofibrosis with myeloid metaplasia: Recognition of different classes of patients by erythrokinetics. Br J Haematol 48:263, 1981 17. Wang JC, Wang A: Plasma soluble interleukin-2 receptor in patients with primary myelofibrosis. Br J Haematol 86:380, 1994 18. Besa EC, Nowell PC, Geller NL, Gardner FM: Analysis of the androgen response of 23 patients with agnogenic myeloid metaplasia. The value of chromosomal studies in predicting response and survival. Cancer 49:308, 1982 19. Reilly S T , Wilson G, Barnett D, Watmore A, Potter A: Karyotypic and ras gene mutational analysis in idiopathic myelofibrosis. Br J Haematol 88575, 1994 20. Barosi G, Ambrosetti A, Buratti A, Finelli C, Liberato NL, Quaglini S, Ricetti MM, Visani G, Tura S, Ascari E: Splenectomy for patients with myelofibrosis with myeloid metaplasia: Pretreatment variables and outcome prediction. Leukemia 7:200, 1993 21. Breslow NE, DayNE: The standardized mortality ratio, in Sen PK Biostatistics (eds): Statistics in Biomedical, Public Health and Environmental Sciences. New York, NY, North-HollandElsevier Science Publishers BV, 1985, p 55 From www.bloodjournal.org by guest on February 6, 2015. For personal use only. 1996 88: 1013-1018 Prognostic factors in agnogenic myeloid metaplasia: a report on 195 cases with a new scoring system [see comments] B Dupriez, P Morel, JL Demory, JL Lai, M Simon, I Plantier and F Bauters Updated information and services can be found at: http://www.bloodjournal.org/content/88/3/1013.full.html Articles on similar topics can be found in the following Blood collections Information about reproducing this article in parts or in its entirety may be found online at: http://www.bloodjournal.org/site/misc/rights.xhtml#repub_requests Information about ordering reprints may be found online at: http://www.bloodjournal.org/site/misc/rights.xhtml#reprints Information about subscriptions and ASH membership may be found online at: http://www.bloodjournal.org/site/subscriptions/index.xhtml Blood (print ISSN 0006-4971, online ISSN 1528-0020), is published weekly by the American Society of Hematology, 2021 L St, NW, Suite 900, Washington DC 20036. 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