1. No category

Research Group on Biotechnology and Reproductive Biology in Woody
Species.
Universities of Santiago de Compostela and Vigo, Spain
Publications
Last compilation made by Manuel Rey, November 25th 2015, sorted chronologically, most
recent first.
Royo, C.; Carbonell-Bejerano, P.; Torres-Pérez, R.; Nebish, A.; Martínez, Ó.; Rey, M.;
Aroutiounian, R.; Ibáñez, J.; Martínez-Zapater, J.M. 2015. Developmental,
transcriptome, and genetic alterations associated with parthenocarpy in the grapevine
seedless somatic variant Corinto bianco. Journal of Experimental Botany (published
online, doi:10.1093/jxb/erv452).
Acanda, Y.; Martínez, Ó.; González, M.V.; Prado, M.J.; Rey, M. 2015. Highly efficient in
vitro tetraploid plant production via colchicine treatment using embryogenic
suspension cultures in grapevine (Vitis vinifera cv. Mencía). Plant Cell, Tissue and
Organ Culture 123: 547-555.
Ferradás, Y.; López, M.; Testillano, P.; Rey, M.; González, M.V. 2015. TUNEL assay in
kiwifruit stigmatic arms. Bio-protocol 5(7): e1434. http://www.bio-protocol.org/
e1434.
Ferradás, Y.; López, M.; Rey, M.; González, M.V. 2014. Programmed cell death in kiwifruit
stigmatic arms and its relationship to the effective pollination period and the progamic
phase. Annals of Botany 114: 35-45.
Prado, M.J.; Largo, A.; Domínguez, C.; González, M.V.; Rey, M.; Centeno, M.L. 2014.
Determination of abscisic acid and its glucosyl ester in embryogenic callus cultures of
Vitis vinifera in relation to the maturation of somatic embryos using a new liquid
chromatography-ELISA analysis method. Journal of Plant Physiology 171: 852-859.
Acanda, Y.; Martínez, Ó.; Prado, M.J.; González, M.V.; Rey, M. 2014. EMS mutagenesis
and qPCR-HRM prescreening for point mutations in an embryogenic cell suspension of
grapevine. Plant Cell Reports 33: 471-481.
Acanda, Y.; Prado, M.J.; González, M.V.; Rey, M. 2013. Somatic embryogenesis from
stamen filaments in grapevine (Vitis vinifera L. cv. Mencía): changes in ploidy level
and nuclear DNA content. In Vitro Cellular and Developmental Biology - Plant 49:
276-284.
González, M.V.; Cuenca, B.; López, M.; Prado, M.J.; Rey, M. 2011. Molecular
characterization of chestnut plants selected for putative resistance to Phytophthora
cinnamomi using SSR markers. Scientia Horticulturae 130: 459-467.
Rey, M. 2010. Vid (Vitis vinifera L., Vitaceae). In: La Biotecnología Aplicada al
Mejoramiento de los Cultivos de Frutas Tropicales. M. Perea-Dallos, L.P. MatallanaRamírez, A. Tirado-Perea, eds. Editorial Universidad Nacional de Colombia, Bogotá,
Colombia. pp: 528-565. ISBN: 978-958-719-536-1.
Prado, M.J.; Grueiro, M.P.; González, M.V.; Testillano, P.S.; Domínguez, C.; López, M.;
Rey, M. 2010. Efficient plant regeneration through somatic embryogenesis from
anthers and ovaries of six autochthonous grapevine cultivars from Galicia (Spain).
Scientia Horticulturae 125: 342-352.
Prado, M.J.; Rodriguez, E.; Rey, L.; González, M.V.; Santos, C.; Rey, M. 2010. Detection
of somaclonal variants in somatic embryogenesis-regenerated plants of Vitis vinifera
by flow cytometry and microsatellite markers. Plant Cell, Tissue and Organ Culture
103: 49-59.
Novo, M.; Romo, S.; Rey, M.; Prado, M.J.; González, M.V. 2010. Identification and
sequence characterisation of molecular markers polymorphic between male kiwifruit
(Actinidia chinensis var. deliciosa (A. Chev.) A. Chev.) accessions exhibiting different
flowering time. Euphytica 175: 109-121.
López, M.; Cid, N.; González, M.V.; Cuenca, B.; Prado, M.J.; Rey, M. 2009. Microsatellite
and AFLP analysis of autochthonous grapevine cultivars from Galicia (Spain). American
Journal of Enology and Viticulture 60(2): 215-222.
Prado, M.J.; González, M.V.; Romo, S.; Herrera, M.T. 2007. Adventitious plant
regeneration on leaf explants from adult male kiwifruit and AFLP analysis of genetic
variation. Plant Cell, Tissue and Organ Culture 88: 1-10.
Prado, M.J.; Romo, S.; Novo, M.; Rey, M.; Herrera, M.T.; González, M.V. 2006. Molecular
characterization of three comercial cultivars and a new pollinator in kiwifruit (A.
deliciosa). HortScience 41: 90-95.
Couselo, J.L.; Varela, P.; Rey, M. 2006. Effect of benzyladenine concentration and doublephase culture system on in vitro multiplication of adult Albariño plants. American
Journal of Enology and Viticulture 57: 109-112.
Prado, M.J.; Herrera, M.T.; Vázquez, R.A.; Romo, S.; González, M.V. 2005.
Micropropagation of two selected males kiwifruit and analysis of genetic variation with
AFLP markers. HortScience 40: 740-746.
Di Gaspero, G.; Cipriani, G.; Marrazzo, M.T.; Andreetta, D.; Prado, M.J.; Testolin, R.
2005. Isolation of (AC)n-microsatellites in Vitis vinifera (L.) and analysis of genetic
background in grapevines under marker assisted selection. Molecular Breeding 15:
11-20.
Bueno, M.A.; Pintos, B.; Prado, M.J.; Gómez, A.; Manzanera, J.A. 2004. Androgenesis: a
tool for woody plant breeding. In: Recent Res. Devel. Genet. Breeding (S.G. Pandalai,
ed.). Vol. 1. pp. 365-383. Research Signpost, Kerala, India.
Ávila, C.; Cantón, F.R.; Barnestein, P.; Suárez, M.-F.; Marraccini, P.; Rey, M.; Humara,
J.M.; Ordás, R.; Cánovas, F.M. 2002. Structural and functional characterization of the
5' upstream region of a glutamine synthetase gene from Scots pine. Annals of Forest
Science 59: 669-673.
Ávila, C.; Cantón, F.R.; Barnestein, P.; Suárez, M.-F.; Marraccini, P.; Rey, M.; Humara,
J.M.; Ordás, R.; Cánovas, F.M. 2001. The promoter of a cytosolic glutamine synthetase
gene from the conifer Pinus sylvestris is active in cotyledons of germinating seeds and
light-regulated in transgenic Arabidopsis thaliana. Physiologia Plantarum 112:
388-396.
Desiderio, A.; Franconi, R.; López, M.; Villani, M.E.; Viti, F.; Chiaraluce, R.; Consalvi, V.;
Neri, D.; Benvenuto, E. 2001. A semi-synthetic repertoire of intrinsically stable
antibody fragments derived from a single-framework scaffold. Journal of Molecular
Biology 310: 603-615.
Humara, J.M.; López, M.; Casares, A.; Majada, J. 2000. Temperature and provenance as
two factors affecting Eucaliptus nitens seed germination. Forestry 73: 87-90.
López, M.; Humara, J.M.; Casares, A.; Majada, J. 2000. The effect of temperature and
water stress on laboratory germination of Eucalyptus globulus Labill. seeds of different
sizes. Annals of Forest Science 57: 245-250.
López, M.; Humara, J.M.; Rodríguez, R.; Ordás, R.J. 2000. Transient uidA gene
expression in electroporated cotyledonary protoplasts of Pinus nigra Arn. ssp.
Salzmannii and in bombarded cotyledons. Canadian Journal of Forest Research 30:
448-445.
López, M.; Humara, J.M.; Rodríguez, R.; Ordás, R.J. 2000. Factors involved in
Agrobacterium tumefaciens-mediated gene transfer into Pinus nigra Arn. Ssp.
Salzmannii (Dunal) Franco. Euphytica 114: 195-203.
González, M.V.; López, M.; Valdés, E.; Ordás, R.J. 2000. Micropropagation of three berry
fruit species using nodal segments from field-grown plants. Annals of Applied Biology
137: 73-78.
Humara, J.M.; López, M.; Ordás, R.J. 1999. Agrobacterium tumefaciens-mediated
transformacion of Pinus pinea L. cotyledons: an assessment of factors influencing the
efficiency of uidA gene transfer. Plant Cell Reports 19: 51-58.
Humara, J.M.; López, M.; Ordás, R.J. 1999. Induction of a virulence response in
Agrobacterium tumefaciens by exudates of Pinus pinea cotyledons. Plant Cell, Tissue
and Organ Culture 55: 175-181.
Humara, J.M.; López, M.; Ordás, R.J. 1999. Transient expression of the uidA gene in
Pinus pinea cotyledons: A study of heterologous promoter sequences. Plant Cell,
Tissue and Organ Culture 56: 69-78.
González, M.V.; Rey, M.; Tavazza, R.; La Malfa, S.; Cuozzo, L.; Ancora, G. 1998. In vitro
adventitious shoot formation on cotyledons of Pinus pinea L. HortScience 33: 749-750.
Humara, J.M.; López, M.; Ordás, R.J. 1998. Modifying transient β-glucuronidase
expression in pine species using introns. Plant Cell, Tissue and Organ Culture 52:
183-187.
Rey, M.; Díaz Sala, C.; Rodríguez, R. 1998. Free polyamine content in leaves and buds of
hazelnut (Corylus avellana L. cv. negret) trees subjected to repeated severe pruning.
Scientia Horticulturae 76: 115-121.
López, M.; Pacheco, J.; Rodríguez, R.; Ordás, R.J. 1996. Regeneration of plants from
isolated cotyledons of black pine (Pinus nigra Arn. ssp. salzmannii (Dunal) Franco. In
Vitro Cellular and Developmental Biology - Plant 32: 109-114.
Rey, M.; González, M.V.; Ordás, R.J.; Ancora, G.; Tavazza, R. 1996. Factors affecting
transient gene expression in cultured radiata pine cotyledons following particle
bombardment. Physiologia Plantarum 96: 630-636.
Berros, B.; Rey, M.; Díaz Sala, C.; Albuerne, M.; Rodríguez, R. 1995. Somatic
embryogenesis in hazelnut (Corylus Species). In: Biotechnology in Agriculture and
Forestry, Vol. 30. Somatic Embryogenesis and Synthetic Seed I. Y.P.S. Bajaj, ed. pp.
318-334. Springer-Verlag, Berlin.
González, M.V.; Rey, M.; Rodríguez, R. 1995. Plant regeneration from petioles of kiwifruit
microshoots. HortScience 30: 1302-1303.
Díaz Sala, C.; Rey, M.; Boronat, A.; Besford, R.T.; Rodríguez, R. 1995. Variations in the
DNA methylation and polypeptide patterns of adult hazel (Corylus avellana L.)
associated with sequential in vitro subcultures. Plant Cell Reports 15: 218-221.
Rey, M.; Tiburcio, A.F.; Díaz Sala, C.; Rodríguez, R. 1994. Endogenous polyamine
concentrations in juvenile, adult and in vitro reinvigorated hazel. Tree Physiology 14:
191-200.
Rey, M.; Díaz Sala, C.; Rodríguez, R. 1994. Comparison of endogenous polyamine
content in hazel leaves and buds between the annual dormancy and flowering phases
of growth. Physiologia Plantarum 91: 45-50.
Rey, M.; Díaz Sala, C.; Rodríguez, R. 1994. Exogenous polyamines improve rooting of
hazel microshoots. Plant Cell, Tissue and Organ Culture 36: 303-308.
Díaz Sala, C.; Rey, M.; Rodríguez, R. 1994. Temporary modification of adult filbert
proliferation capacity by sequential subcultures: intensive pruning as a pretreatment
for in vitro reinvigoration. Journal of Horticultural Science 69: 673-678.
Rey, M.; Díaz Sala, C.; Rodríguez, R. 1994. Effect of repeated severe pruning on
endogenous polyamine content in hazelnut trees. Physiologia Plantarum 92: 487-492.
Revilla, M.A.; Rey, M.; González Rio, F.; González, M.V.; Díaz Sala, C.; Rodríguez, R.
1992. Micropropagation of kiwi (Actinidia spp.). In: Biotechnology in Agriculture and
Forestry, Vol. 18. High Tech and Micropropagation II. Y.P.S. Bajaj, ed. pp. 399-423.
Springer-Verlag, Berlin.
Rey, M.; Fernández, M.T.; González, V.; Rodríguez, R. 1992. Kiwifruit micropropagation
through callus shoot-bud induction. In Vitro Cellular and Developmental Biology 28P:
148-152.
Díaz Sala, C.; Rey, M.; Rodríguez, R. 1990. In vitro establishment of a cycloclonal chain
from nodal segments and apical buds of adult hazel (Corylus avellana L.). Plant Cell,
Tissue and Organ Culture 23: 151-157.
Rodríguez, R.; Rey, M.; Cuozzo, L.; Ancora, G. 1990. In vitro propagation of caper
(Capparis spinosa L.). In Vitro Cellular and Developmental Biology 26: 531-536.