1. Ingeniería

Scientific Instrumentation Available for Use by the Research Community
(updated 1/29/15)
Instrument
Flow Cytometry
Advanced Flow Cytometry Analyzers
Manufacturer/Model
Location
Access requirements
BD LSR II (2 instruments)
BD LSR-Fortessa (1 instrument)
FLOW CYTOMETRY RESOURCE
CENTER (DWB 211)
Basic Flow Cytometry Analyzers
BD Accuri C6 (1 instrument)
FLOW CYTOMETRY RESOURCE
CENTER (DWB 211)
Flow Cytometer analyzer
BD LSR II (5 lasers)
Translational Technology Core
Laboratory (TTCL) (RU Hospital 234)
Completion of appropriate training.
FLOW CYTOMETRY RESOURCE
CENTER on-line booking system
registration is needed. Cardkey
access; 24/7
Completion of appropriate training.
FLOW CYTOMETRY RESOURCE
CENTER on-line booking system
registration is needed. Cardkey
access; 24/7
Training by the TTCL. Reservation on
the TTCL booking system
Olympus IX71 microscope
BIO-IMAGING RESOURCE CENTER
(DWB 201)
Completion of appropriate training;
cardkey access 24/7
CRI Nuance camera on CRI Nuance
camera on Olympus inverted
microscope
Olympus FV1000MPE Twin system
with Coherent Vision II and XR IR
lasers
Applied Precision Inc. OMX Blaze
BIO-IMAGING RESOURCE CENTER
(DWB 201)
Completion of appropriate training;
cardkey access 24/7
BIO-IMAGING RESOURCE CENTER
(DWB 202)
Completion of appropriate training;
cardkey access 24/7
BIO-IMAGING RESOURCE CENTER
(DWB 202)
BIO-IMAGING RESOURCE CENTER
(DWB 202)
Completion of appropriate training;
cardkey access 24/7
Completion of appropriate training;
cardkey access 24/7
BIO-IMAGING RESOURCE CENTER
(DWB 201)
BIO-IMAGING RESOURCE CENTER
(DWB 201)
BIO-IMAGING RESOURCE CENTER
(DWB 202)
Completion of appropriate training;
cardkey access 24/7
Completion of appropriate training;
cardkey access 24/7
Completion of appropriate training;
cardkey access 24/7
BIO-IMAGING RESOURCE CENTER
(DWB 202)
BIO-IMAGING RESOURCE CENTER
(DWB 201)
Completion of appropriate training;
cardkey access 24/7
Completion of appropriate training;
cardkey access 24/7
Light Microscopy
Inverted light microscope with B/W
and color digital cameras, also fitted
with phase contrast optics
Widefield spectral imaging system
Upright multiphoton system
3D-SIM super-resolution microscopy
system
STORM/TIRF inverted microscope
system also fitted with FLIM system
Wide-field fluorescence/brightfield/DIC
microscope
Inverted fluorescence microscope for
tissue culture cells
CellVoyager spinning disk microscope
system with full environmental control
and multi-well dish holders
DeltaVision Image Restoration
Microscope
Inverted laser scanning confocal
microscope
Nikon and Lambert
Instruments/STORM/TIRF/FLIM
Microscope
Zeiss, Axioplan 2 imaging upright
Nikon Eclipse TS100 inverted
Olympus/Yokagawa CV1000
Applied Precision / Olympus; Inverted
Olympus IX-70 microscope (2)
Leica SP8
Scientific Instrumentation Available for Use by the Research Community
(updated 1/29/15)
Inverted laser scanning confocal
microscope
Inverted laser scanning confocal
microscope
Light sheet microscope for imaging
cleared samples
Spinning disk confocal microscope for
real-time live cell imaging
Inverted multiphoton microscope
system
Laser microdissection system
Zeiss LSM 510
Incubator microscope for long-term
live cell imaging with full
environmental control
Live animal imaging
Olympus LCV110U VivaView
BIO-IMAGING RESOURCE CENTER
(DWB 201)
BIO-IMAGING RESOURCE CENTER
(DWB 203)
BIO-IMAGING RESOURCE CENTER
(DWB 202)
BIO-IMAGING RESOURCE CENTER
(DWB 201)
BIO-IMAGING RESOURCE CENTER
(DWB 201)
BIO-IMAGING RESOURCE CENTER
(DWB 202)
BIO-IMAGING RESOURCE CENTER
- DWB 202
Completion of appropriate training;
cardkey access 24/7
Completion of appropriate training;
cardkey access 24/7
Completion of appropriate training;
cardkey access 24/7
Completion of appropriate training;
cardkey access 24/7
Completion of appropriate training;
cardkey access 24/7
Completion of appropriate training;
cardkey access 24/7
Completion of appropriate training;
cardkey access 24/7
IVIS Illumina
COMPARATIVE BIOSCIENCE
CENTER (CBC)
UV microscope for protein crystal
inspection
Inverted microscope with digital
camera
Electron Microscopy
Transmission electron microscope
JANSi UVEX
STRUCTURAL BIOLOGY
RESOURCE CENTER (RRB 363)
Translational Technology Core
Laboratory (RU Hospital 234)
ACUC protocol approval; Completion
of appropriate training; cardkey
access 24/7
Cardkey access only after hours
Transmission electron microscope
Transmission electron microscopes
Scanning electron microscope
Scanning electron microscope
Cryo-plunger
Zeiss LSM 780
LaVision Ultramicroscope
Zeiss/Perkin-Elmer; Inverted Zeiss
Axiovert 200 microscope
Zeiss LSM 510 NLO
MMI CellCut system
Zeiss/Axiovert 40 with Variable Relief
Contrast (VAREL)
FEI Tecnai G2 Spirit BioTwin with
Gatan 4K x 4K digital camera, Low
dose, Cryo-EM and Serial EMA
JEOL 100 cx with AMT 2K x 2K digital
imaging system
ELECTRON MICROSCOPY
RESOURCE CENTER (RRB 152)
JEOL 1400 Plus with Gatan 2K x 2K
digital imaging system, EDS (Energydispersive X-ray spectroscopy), Low
dose, Cryo-EM and Serial EMA
ZEISS LEO 1550 with field-emission
electron gun, Backscatter Detector
ELECTRON MICROSCOPY
RESOURCE CENTER (RRB 120)
ZEISS Merlin with field-emission
electron gun, 3View, CLEM shuttle
system
FEI Vitrobot™ Mark IV – Automated
ELECTRON MICROSCOPY
RESOURCE CENTER (RRB 154A)
ELECTRON MICROSCOPY
RESOURCE CENTER (RRB 120)
ELECTRON MICROSCOPY
RESOURCE CENTER (RRB 154)
ELECTRON MICROSCOPY
Reservation on the TTCL booking
system
Upon approval by Electron
Microscopy Center Director and with
appropriate scheduling
Upon approval by Electron
Microscopy Center Director and with
appropriate scheduling
Upon approval by Electron
Microscopy Center Director and with
appropriate scheduling
Upon approval by Electron
Microscopy Center Director and with
appropriate scheduling
To be commissioned.
Upon approval by Electron
Scientific Instrumentation Available for Use by the Research Community
(updated 1/29/15)
sample vitrification
RESOURCE CENTER (RRB 120)
Ultramicrotome
REICHELT Ultra Cut E
ultramicrotomes
ELECTRON MICROSCOPY
RESOURCE CENTER (RRB 120)
Cryo-ultramicrotome
LEICA UC6b/EM FC6
cryoultramicrotome
ELECTRON MICROSCOPY
RESOURCE CENTER (RRB 120)
Glass knife-makers
LKB glass knife-makers
ELECTRON MICROSCOPY
RESOURCE CENTER (RRB 120)
Vacuum Evaporator
EDWARDS Auto 306
High Pressure Freezer
LEICA High Pressure Freezer EMPACT2
LEICA EM AFS2 Automatic Freeze
Substitution System
LEICA EM TP Automatic Tissue
Processor
PELCO BioWave® Pro-laboratory
microwave system
ELECTRON MICROSCOPY
RESOURCE CENTER (RRB 120)
ELECTRON MICROSCOPY
RESOURCE CENTER (RRB 120)
ELECTRON MICROSCOPY
RESOURCE CENTER (RRB 120)
ELECTRON MICROSCOPY
RESOURCE CENTER (RRB 120)
ELECTRON MICROSCOPY
RESOURCE CENTER (RRB 120)
Epi-fluorescent Microscope
NIKON EclipseTi – with Nikon CCD
digital camera
ELECTRON MICROSCOPY
RESOURCE CENTER (RRB 120)
Glow Discharger
GD110
ELECTRON MICROSCOPY
RESOURCE CENTER (RRB 120)
Computer Analysis Tablet
WACOM Cintiq
ELECTRON MICROSCOPY
RESOURCE CENTER (RRB 120)
High Vacuum Coater for SEM
LEICA EM TP Automatic Tissue
Processor
ELECTRON MICROSCOPY
RESOURCE CENTER (RRB 120)
Vibrating Blade Microtome
LEICA VT100S
ELECTRON MICROSCOPY
RESOURCE CENTER (RRB 120)
Calorimeter
Isothermal Calorimeter with micro titer
plate auto-sample
GE MicroCal MicrolTC
HIGH THROUGHPUT SCREENING
RESOURCE CENTER (DWB
Freeze Substitution System
EM Tissue Processor
EM Microwave Processor
Microscopy Center Director and with
appropriate scheduling
Upon approval by Electron
Microscopy Center Director and with
appropriate scheduling
Upon approval by Electron
Microscopy Center Director and with
appropriate scheduling
Upon approval by Electron
Microscopy Center Director and with
appropriate scheduling
By Appointment
By Appointment
By Appointment
By Appointment
Upon approval by Electron
Microscopy Center Director and EMproject with appropriate scheduling
Upon approval by Electron
Microscopy Center Director and EMproject with appropriate scheduling
Upon approval by Electron
Microscopy Center Director and EMproject with appropriate scheduling
Upon approval by Electron
Microscopy Center Director and EMproject with appropriate scheduling
Upon approval by Electron
Microscopy Center Director and EMproject with appropriate scheduling
Upon approval by Electron
Microscopy Center Director and EMproject with appropriate scheduling
Completion of appropriate training;
cardkey access 24/7
Scientific Instrumentation Available for Use by the Research Community
(updated 1/29/15)
210/214/216)
Chromotography
HPLC
Waters HPLC setup with auto sampler
FPLC
Amersham AKTA System
Off-gel electrophoresis
3100 OFFGEL Fractionator
Computing
Image processing – LSM 510
Image processing – SoftWoRx
Image processing – Imaris
Image processing – Huygens
Image processing – MetaMorph
Image processing – Circusoft
Image processing – Adobe products
Image processing – Red Hat Linux
Cell analysis workstations
"LSM 510 v. 3.2" confocal software
(Zeiss) (projections, movies, ROI
measurements, intensity profiles)
"SoftWoRx" (Applied Precision /
DeltaVision) (deconvolution, volume
rendering, surface rendering,
quantification)
"Imaris" "Surpass" and
"FilamentTracer" (Bitplane) (volume
rendering in 3D and 4D, surface
rendering, measurements,
colocalization, clipping planes, 4D
tracking, filament tracing)
“Huygens" deconvolution software
(SVI) (modules for deconvolving
confocal, spinning disk or multiphoton
data)
"MetaMorph" (Universal Imaging)
(cell counting, tracking,
measurements, kymographs)
"PFRET" software (Circusoft)
(sensitized emission FRET analysis)
Adobe Photoshop and Adobe
Premiere
One linux workstation (using Red Hat
linux) is available for deconvolving
and processing images collected on
the DeltaVision microscope.
FLOW CYTOMETRY RESOURCE
CENTER Analysis I, II and PC
PROTEOMICS RESOURCE CENTER
- (RRB 156)
PROTEOMICS RESOURCE CENTER
- (RRB 156)
PROTEOMICS RESOURCE CENTER
- (RRB 151)
Completion of appropriate training
BIO-IMAGING RESOURCE CENTER
(DWB 203)
Completion of appropriate training;
cardkey access 24/7
BIO-IMAGING RESOURCE CENTER
(DWB 203)
Completion of appropriate training;
cardkey access 24/7
BIO-IMAGING RESOURCE CENTER
(DWB 203)
Completion of appropriate training;
cardkey access 24/7
BIO-IMAGING RESOURCE CENTER
(DWB 203)
Completion of appropriate training;
cardkey access 24/7
BIO-IMAGING RESOURCE CENTER
(DWB 203)
Completion of appropriate training;
cardkey access 24/7
BIO-IMAGING RESOURCE CENTER
(DWB 203)
BIO-IMAGING RESOURCE CENTER
(DWB 203)
BIO-IMAGING RESOURCE CENTER
(DWB 203)
Completion of appropriate training;
cardkey access 24/7
Completion of appropriate training;
cardkey access 24/7
Completion of appropriate training;
cardkey access 24/7
FLOW CYTOMETRY RESOURCE
CENTER (DWB 202A)
Both FLOW CYTOMETRY
RESOURCE CENTER on-line
booking system registration and CFS
Completion of appropriate training;
cardkey access 24/7
Completion of appropriate training
Scientific Instrumentation Available for Use by the Research Community
(updated 1/29/15)
Microarray data analysis
GeneSpring
Realtime PCR data analysis
Sequence detection system (SDS)
Irradiators
Cell irradiator
Nordian Cesium cell irradiator
DWB 1 floor
Animal X-ray irradiator
RadSource RS-2000
COMPARATIVE BIOSCIENCES
nd
CENTER 2 floor
Liquid Handling
Microtiter plate washer with stacker
Biotek EL406
HIGH THROUGHPUT SCREENING
RESOURCE CENTER (DWB
210/214/216)
HIGH THROUGHPUT SCREENING
RESOURCE CENTER (DWB
210/214/216)
HIGH THROUGHPUT SCREENING
RESOURCE CENTER (DWB
210/214/216)
STRUCTURAL BIOLOGY
RESOURCE CENTER (RRB 363)
STRUCTURAL BIOLOGY
RESOURCE CENTER (RRB 363)
Completion of appropriate training;
cardkey access 24/7
TEDA charcoal filtered hood
LKB WALLAC Beta Liquid Scintillation
Counter, Model 1209
Packard Gamma Counter Model 5530
Various handheld models
Various handheld models
LS&EH (WRB A13)
LS&EH (FRS A8)
By appointment with LS&EH
24/7
LS&EH (FRS A8)
LS&EH (FRS A8)
LS&EH (FRS A8)
24/7
For loan from LS&EH
For loan from LS&EH
NanoDrop -2000
GENOMICS RESOURCE CENTER
(WRB 723)
GENOMICS RESOURCE CENTER
2- minute training; cardkey access
24/7
Brief training; cardkey access 24/7
Automated High Throughput Pipetting
Station
Tecan EVO
Microtiter plate liquid dispenser and
stacker
Thermo MultiDrop Combi with
Rapidstack
Formulator 96,24-well liquid handler
Formulatrix
Phoenix 96-well nano dispenser
Art Robbins
Radioactive Materials Handling
Iodination fume hood
Beta scintillation counter
Gamma counter
Survey meter (beta)
Survey meter
Sample Quantitation
Nucleic acid quantitation
Nucleic acid quantitation
NanoDrop-8000
GENOMICS RESOURCE CENTER
(WRB 723)
GENOMICS RESOURCE CENTER
(WRB 723)
login/password are needed. Cardkey
access 24/7
Cardkey access 24/7
st
Cardkey access 24/7
Registered and approved users only;
cardkey access, 24/7 (for information,
contact [email protected])
As part of approved IACUC protocol
only; scheduling and access via CBC
staff in the main office; training
through CBC Vet Services Supervisor
(ext. 8526)
Completion of appropriate training;
cardkey access 24/7
Completion of appropriate training;
cardkey access 24/7
Completion of appropriate training;
cardkey access only after hours
Completion of appropriate training;
cardkey access only after hours
Scientific Instrumentation Available for Use by the Research Community
(updated 1/29/15)
(WRB 723)
Plate Reader
Luminometer
96 well plate luminometer
Multi-function microtiter plate reader
with stacker
Biotek Synergy Neo
Fluorescence microtiter plate reader
with built-in dispensing and stacker
Hammamatsu FDSS 6000
Microtiter plate reader Scintillation and
luminescence
Perkin-Elmer TriLux
Fluorescence microscope plate reader
Molecular Devices ImageXpress Micro
InfraRed Dye Scanner
LICOR Odyssey SA
Laser scanning plate reader
Molecular Devices Imagexpress Velo
Absorbance/luminescence/
fluorescence
BMG Labtechnology/FLUOstar
Omega; 6-1536-well plates
ANTIBODY AND BIORESOURCE
CORE FACILITY (MSK)
HIGH THROUGHPUT SCREENING
RESOURCE CENTER (DWB
210/214/216)
HIGH THROUGHPUT SCREENING
RESOURCE CENTER (DWB
210/214/216)
HIGH THROUGHPUT SCREENING
RESOURCE CENTER (DWB
210/214/216)
HIGH THROUGHPUT SCREENING
RESOURCE CENTER (DWB
210/214/216)
HIGH THROUGHPUT SCREENING
RESOURCE CENTER (DWB
210/214/216)
HIGH THROUGHPUT SCREENING
RESOURCE CENTER (DWB
210/214/216)
Translational Technology Core
Laboratory (RU Hospital 234)
By arrangement with Manager
Multianalyte profiling (xMAP tech).
Multiplex assay, absolute
quantification of biomarkers ( s/n,
serum, plasma, sf, urine, etc.)
Electrochemoluminescence Multiplex
assay. Absolute quantifcation of
biomarkers (s/n, serum, plasma, sf,
urine, etc..)
Single-molecule counting tech.
Singleplex assay, absolute
quantication of biomarkers (s/n, sf,
serum, urine, etc..)
PCR Instrument
PCR
Luminex 200.
96-well plate
Translational Technology Core
Laboratory (RU Hospital 234)
Training by the TTCL.
Reservation on the TTCL booking
system
Training by the TTCL. Reservation on
the TTCL booking system
MSD/Sector Imager 2400A.
96-well plate
Translational Technology Core
Laboratory (RU Hospital 234)
Training by the TTCL. Reservation on
the TTCL booking system
Singulex/Erenna Immunoassay
System, high sensitivity (fg/ml)
Translational Technology Core
Laboratory (RU Hospital 234)
Training by the TTCL. Reservation on
the TTCL booking system
Roche LightCycler 480
GENOMICS RESOURCE CENTER
(WRB 723)
Completion of appropriate training;
cardkey access 24/7
Completion of appropriate training;
cardkey access 24/7
Completion of appropriate training;
cardkey access 24/7
Completion of appropriate training;
cardkey access 24/7
Completion of appropriate training;
cardkey access 24/7
Completion of appropriate training;
cardkey access 24/7
Completion of appropriate training;
cardkey access 24/7
Scientific Instrumentation Available for Use by the Research Community
(updated 1/29/15)
PCR
GeneAmp PCR System 9700 (for 96well and 384-well plates)
Lifetechnologies/7900HT
96-well plate
Lifetechnologies/QuantoStudio 3D
GENOMICS RESOURCE CENTER
(WRB 723)
Translational Technology Core
Laboratory (RU Hospital 234)
Translational Technology Core
Laboratory (RU Hospital 234)
Cardkey access 24/7
Bruker Avance 600 MHz
SPECTROSCOPY RESOURCE
CENTER (TSH B16)
SPECTROSCOPY RESOURCE
CENTER (TSH S509)
HIGH THROUGHPUT SCREENING
RESOURCE CENTER (DWB 208)
HIGH THROUGHPUT SCREENING
RESOURCE CENTER (DWB 208)
HIGH THROUGHPUT SCREENING
RESOURCE CENTER (DWB 208)
PROTEOMICS RESOURCE CENTER
(RRB 151)
Cardkey access 24/7
STRUCTURAL BIOLOGY
RESOURCE CENTER (RRB 363)
STRUCTURAL BIOLOGY
RESOURCE CENTER (RRB 363)
STRUCTURAL BIOLOGY
RESOURCE CENTER (RRB 363)
STRUCTURAL BIOLOGY
RESOURCE CENTER (RRB 327)
Completion of appropriate training;
cardkey access only after hours
Completion of appropriate training;
cardkey access only after hours
Completion of appropriate training;
cardkey access only after hours
Completion of appropriate training;
cardkey access only after hours
By arrangement with the BIOIMAGING RESOURCE CENTER
Senior Director
By arrangement with HIGH
THROUGHPUT SCREENING
RESOURCE CENTER Director
By arrangement with the BIOIMAGING RESOURCE CENTER
qRT PCR
Digital qRT-PCR; Absolute
Quantification (mRNA copies, viral
load, mutations
Spectroscopy
NMR Spectrometer
NMR Spectrometer
Bruker DPX 400 MHz
Thermophoresis
NanoTemper MST
Surface Plasmon Resonance
Bio-Rad Proteon XPR
Circular Dichroism Spectrometer
Aviv 202 Circular Dichroism
Spectrometer
PerSeptive Voyager DE STR MALDITOF Mass Spectrometer
Mass Spectrometer
X-ray Diffraction
Microscope, UV for crystal screening
JanScientific
Formulator 24,96-well liquid handler
Formulatrix
Phoenix 96-well nano dispenser
Art Robbins
Macromolecular Crystallography
Rigaku/MSC microMax 007
HF or HR optics with RaxisIV++
imageplate
Training by the TTCL. Reservation on
the TTCL booking system
Training by the TTCL. Reservation on
the TTCL booking system
Cardkey access 24/7
Cardkey access 24/7
Completion of appropriate training;
cardkey access 24/7
Completion of appropriate training;
cardkey access 24/7
Completion of appropriate training;
cardkey access 24/7
Tissue Culture/Animal Prep
Biological Safety Cabinet
Baker Class II 4’- For live animal and
live cell imaging prep work
BIO-IMAGING RESOURCE CENTER
(DWB 201)
Biological Safety Cabinet
Baker SterilGuard
HIGH THROUGHPUT SCREENING
RESOURCE CENTER (DWB 208)
Biological Safety Cabinet
Baker Class II 4’- For live animal and
live cell imaging prep work
BIO-IMAGING RESOURCE CENTER
(DWB 202B)
Scientific Instrumentation Available for Use by the Research Community
(updated 1/29/15)
Incubator with CO2
For temp storage of live cell samples
for imaging
BIO-IMAGING RESOURCE CENTER
(DWB 201)
Incubator without CO2
For temp storage of live cell samples
for imaging
BIO-IMAGING RESOURCE CENTER
(DWB 201)
PROTEOMICS RESOURCE CENTER
(RRB 156)
PROTEOMICS RESOURCE CENTER
(RRB 156)
Completion of appropriate training;
cardkey access 24/7
Completion of appropriate training;
cardkey access 24/7
PROTEOMICS RESOURCE CENTER
(RRB 156)
Completion of appropriate training;
cardkey access 24/7
InfraRed Laser Scanner
Amersham Biosciences Typhoon
9400 Variable Imager
Pharmacia Multiphor II Isoelectric
focusing system (available but no
expertise in center)
Pharmacia Ettan DALTsix
electrophoresis system (available but
no expertise in center)
LiCor Odyssey SA
HIGH THROUGHPUT SCREENING
RESOURCE CENTER (DWB 208)
Completion of appropriate training;
cardkey access 24/7
Sonicator
Sonicator
Covaris S2
GENOMICS RESOURCE CENTER
(WRB 723)
Completion of appropriate training;
cardkey access 24/7
Agilent High Resolution DNA
Microarray Scanner
GENOMICS RESOURCE CENTER
(WRB 729)
Completion of appropriate training;
cardkey access 24/7
PerkinElmer, IVIS Lumina 1
COMPARATIVE BIOSCIENCE
CENTER (CBC vivarium)
Completion of appropriate training;
cardkey access 24/7
Imager and Electrophoresis Units
Fluorescence /phosphor
autoradiography /chemiluminescence
Isoelectric focusing system
Electrophoresis system
Scanner
Glass Slide Scanner
In vivo Imaging
IVIS Lumina in vivo fluorescent
imaging system
Senior Director
By arrangement with the BIOIMAGING RESOURCE CENTER
Senior Director
By arrangement with the BIOIMAGING RESOURCE CENTER
Senior Director