Pres.
C. M. Rick TGRC A genebank of wild species, mutants, and genetic stocks of tomato Tomato Genetics Resource Center Wild species •17 Solanum species •1,200 accessions (populations) Mutants •Single or multiple mutations •Morphology, disease resistance, … •Isogenic or near isogenic control old gold Miscellaneous stocks •Introgression lines •Recombinant inbred lines •Cytogenetic stocks •Latin American landraces •Vintage cultivars Total = 3,839 accessions landraces from Peru New collections of wild tomatoes are limited Access to genetic resources is restricted by international treaties: •1993 – Convention on Biological Diversity •2004 – Intl. Treaty on Plant Genetic Resources for Food and Agric. •2014 – Nagoya Protocol Impact on tomato genetic resources: •No collections of wild tomatoes from Peru or Ecuador have been authorized since passage of the CBD. •Collections made after 1992 are no longer available. The Rick Center’s wild species collection is effectively a non-renewable resource. Wild tomatoes are disappearing from the native region Intensive agriculture Urban expansion Ecuador Peru Mining Grazing Chile Chile C. M. Rick TGRC Tomato Genetics Resource Center Seed Processing Seed of inactive accessions was retrieved from storage Formerly inactive wild species accessions now available S. peruvianum complex S. chilense LA1931 LA1962 LA2729 LA2743 LA2881 LA2947 LA2957 LA2965 Minas de Acari, Arequipa, Peru Huaico Tacna, Tacna, Peru Pacahua quebrada, Tarapaca, Chile La Puntilla, Arica y Parinacota, Chile Quebrada Socaire, Antofagasta, Chile Guatacondo, Tarapaca, Chile Pozo, Tarapaca, Chile Pachia, Tacna, Peru S. habrochaites LA1986 LA1761 LA2541 LA2723 LA2728 LA2859 LA2868 Casmiche, Libertad, Peru Rio Chillon, Lima, Peru Olmos-Jaen, Lambayeque, Peru Yauyos, Lima, Peru Las Juntas, Loja, Ecuador Yangana, Loja, Ecuador Arenillas, El Oro, Ecuador S. pennellii LA1523 LA1773 LA2719 LA2720 Irrigacion Santa Rosa, Lima, Peru Rio Casma, Ancash, Peru Santa Rosa, Lima, Peru Pacaran-Catahuasi, Lima, Peru LA1952 LA2957B LA2958 LA1979 LA2813 LA3157 Sper Sper Sper Shua Sarc Scor Lomas de Camana, Arequipa, Peru Pozo, Tarapaca, Chile Caleta Vitor, Arica y Parinacota, Chile Colca (Rio Fortaleza), Ancash, Peru San Juan, Cajamarca, Peru Quebrada Tinajas, Lima, Peru S. pimpinellifolium LA2534 LA2535 LA2536 LA2537 LA2538 LA2539 LA2540 LA2543 LA2544 LA2545 LA2546 LA2547 LA2549 LA2649 Vista Florida, Lambayeque, Peru Patapo, Lambayeque, Peru Patapo, Lambayeque, Peru Malpaso, Lambayeque, Peru Malpaso, Lambayeque, Peru Cuculi, Lambayeque, Peru La Cria, Lambayeque, Peru Pacanguilla-Pacasmayo, La Libertad, Peru Pacasmayo, La Libertad, Peru Tembladera, Cajamarca, Peru Tonan, Cajamarca, Peru Yatahual, Cajamarca, Peru La Muyuna, Cajamarca, Peru Chulucanas, Piura, Peru S. habrochaites -- Rio Moche, La Libertad, Peru Large flowers, fruit S. habrochaites -- Arenillas, El Oro, Ecuador Only SI accession from Ecuador ★ S. pennellii -- Rio Casma, Ancash, Peru Pedicel joint in ‘mid’ position Tomato-like Solanum species photo CM Rick S. lycopersicoides S. sitiens S. juglandifolium S. ochranthum Albrecht et al. 2010 Ann. Bot. 105: 535 S. sitiens, xerophyte of the Atacama Desert S. sitiens, xerophyte of the Atacama Desert Plant Breeding program project: “An optimized introgression line library and transcriptome sequence of Solanum sitiens: novel resources for tomato breeding and research.” collaborators: Luca Comai, Allen VanDeynze, Hamid Ashrafi 9/2013 – 8/2017 Diana Burkart-Waco Project Goals 1) 2) 3) Develop a library of introgression lines representing the genome of Solanum sitiens in cultivated tomato. Genotype the introgression lines to high resolution. Sequence and analyze the transcriptome of S. sitiens. S. sitiens chromosome segments bred into tomato 1 2 3 0 TG608 4 9 TG301 20 TG184 18 T1706 32 5g49480 35 2g35130 46 54 3g06050 SSR135 48 SSR349A 63 4g30930 69 70 2g45620 TG460 79 4g3556 90 U146494 110 112 SSR125 3g02300 123 TG507 130 1g80460 143 1g67730 145 3g18520 168 5g49830 3g12300 92 116 1g02560 159 163 SSR288 U220487 1g60440 1g26945 SSR325 35 2g01275 51 4g24830 T1601 69 5g05270 83 108 27 2g20390 53 71 1g29520 1g02140 72 1g71810 90 3g48330 103 4g33350 120 1g19600 137 1g76080 7 2 4g30580 0 2 5g46630 4g17380 25 5g05690 29 4g33250 21 23 5g27390 2g25950 39 1g77470 32 5g11490 45 54 2g42810 1g53670 55 56 SSR38 5g47390 75 5g54310 77 1g63980 90 1g55870 87 CT68 108 5g53130 50 5g61910 67 2g43360 83 1g18640 97 U146140 10 2g37240 3 5g06430 2g32600 1 1 TG303 17 34 3g10070 34 5g04910 45 2g38025 61 3g58470 72 2g37510 1g07310 85 3g23400 97 3g24050 103 SSR599 116 T1065 8 5g61510 1 75 1g28530 CAPSS3-2F 7 3g55360 9 2g01770 21 28 6 5 0 18 19 7 86 11 1 12 TG497 14 3g52180 13 4g03280 26 T0408 5g19690 38 41 3g02870 1g21690 27 33 56 5g04590 74 T0801 82 2g28250 93 5g38530 103 5g51700 115 118 3g17000 T0800 2g06530 TG233 allopolyploid crosses diploid crosses S. lycopersicum × S. pimpinellifolium RILs •148 Recombinant inbred lines donated by M. Foolad •F8 generation by SSD from F2’s •Described by Ashrafi et al. 2009 Genome 52: 935 •GBS to be done by A. VanDeynze’s group NC EBR-1 Gardner 1988 S. pimpinellifolium LA2093 La Union, El Oro, Ecuador Traits measured in RILs during seed multiplication Flower / infloresence traits •Stigma exsertion •Anther length •Corolla diameter •# of corolla segments •Inflorescence length •# inflorescence branches •# flowers per inflorescence Fruit traits •# of locules •Shoulder color (u or +) •Fruit weight Other traits •Seed weight •Seed per fruit •Growth habit (sp or +) Stigma Exsertion (mm) 2.00 1.00 NC EBR-1 0.00 LA2093 -1.00 -2.00 -3.00 -4.00 Seed per Fruit 90.00 80.00 70.00 60.00 NC EBR-1 50.00 40.00 30.00 20.00 10.00 0.00 LA2093 Locule Number 4.50 NC EBR-1 4.00 3.50 3.00 2.50 2.00 1.50 1.00 0.50 0.00 LA2093 Seed Weight (g/100 seed) 0.35 NC EBR-1 0.30 0.25 0.20 LA2093 0.15 0.10 0.05 0.00 Thanks to … Diana Burkart-Waco Scott Peacock Christine Nguyen Wentao Li Mira Markova Tom Starbuck Adryanna Corral Kristine Donahue Daniel Short Angela Prada-Baez Matthew Valle Yosuke Moritama Jennifer Petersen Xiuwen Huo Ricardo Pertuze Tim Wills Luca Comai Allen VanDeynze Hamid Ashrafi Department of Plant Sciences C. M. Rick TGRC Tomato Genetics Resource Center Endowment Fundraising Drive •The goal: to raise $1 million in donations to the Rick Center endowment. •To provide stable funding for optimal long-term stewardship of the collection. •For more information: [email protected]
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