1. Healthcare

Date: 11-12 March, 2015
Venue: JNCSAR, Bangalore
www.jncasr.ac.in
www.flowsols.com
Workshop on Flow Cytometry
Experimental Designing and Data
Analysis
11-12 March, 2015
Jawaharlal Nehru Centre for Advanced Scientific
Research (JNCASR), Bangalore
Fluorescent Activated Cell Sorting (FACS) or Flow
Cytometry is widely used in basic and clinical
research. To harness the real power of this state of art
technique, appropriate experimental designing and
accurate analysis of FACS data are crucial. Correct
data analysis is not only important but also critical to
adequately identify or functionally characterize the
multiple populations of interest within the system. In
the last few years, the number of parameters (colors)
simultaneously used in flow cytometry experiments
has increased. This is enabled by the availability of
high-end instruments and powerful data analysis tools.
In this workshop, we will cover analysis of single &
multicolor flow cytometry data and guidelines for the
correct data analysis. Participants will be able to open
their FCS files with the offline data analysis software,
explore their data using various display options,
perform gating, and quickly generate graphical and
statistical reports to identify various populations of
interest. Potential technical issues with the data will
also be discussed. Study material will be provided that
will include lectures, published articles and Purdue
cytometry list discussions.
Eligibility: Graduate
students, postdoctoral
researchers and other
researchers from academic and
non-academic institutions with
prior experience in flow
cytometry are invited for this
course.
Registration: The tuition fee
for the course is Rs. 3,500 for
academic and Rs. 6500 for nonacademic participants. Pay in
cheque/ DD/online transfer
How to apply:
Those who are interested in
attending the course may send
in the following documents to;
[email protected]
/[email protected]
1. Resume
2. Previous experience with
flow cytometry
3. Reason for taking this
course
4. Contact details
Last date of application:
02 March, 2015
Participants will be notified of
their selection by 6th March,
2015.
Travel/ accommodation:
Guest house is available for
outstation candidates on paid
basis.
PROGRAMME OUTLINE
www.jncasr.ac.in
www.flowsols.com
What is flow cytometry and
its application?
Introduction to flow cytometry and
some of the many applications of
flow cytometry
Resource persons:
Dr. Hemant Agrawal, Instructor, Director,
Flowcytometry Solutions Pvt. Ltd.
Prof. Uday Kumar Ranga, HIV-AIDS Lab,
JNCASR, Bangalore
Contact:
Ms. Swaroopa Yalla
Flow Facility Incharge, JNCASR, Bangalore
Ph. No: +91-8494806079, +91-9176249644
E-mail ID: [email protected]
How to design multicolour
flow cytometry experiment?
An overview of experimental
planning and optimization
How to perform right
compensation and its
importance in data
analysis?
Detailed overview of compensation
and its importance in data analysis.
How to make sense of flow
cytometry data?
An overview of correct data
analysis
How to report and present
flow cytometry data?
Discussion about fundamental
statistics and introduction to
MiFlowCyte: Data presentation
guidelines
How to analyze various data
sets?
Demonstration and practice of data
analysis for Immunophenotyping,
Cell Cycle, Proliferation, Apoptosis,
Viability Assay, Population
comparison etc
How to troubleshoot flow
cytometry data?
Guidelines to recognize and
overcome bad data generation
www.jncasr.ac.in
PROGRAMME
DAY 1
9:30 - 10:30: Introduction to Flow
cytometry and its applications
10:30 - 11:30: Designing of a
Multicolor Flow Cytometry
Experiment
- How to avoid Garbage In/Garbage Out
Phenomenon
- Know your instrument.
- Selection of colors (Panel
design)/selection of right antibodies
- Importance of dead cell marker.
- Experimental controls, Isotype
controls, FMO, Biological controls,
Internal controls
- Introduction to OMIPs—Orchestrating
Multiplexity in Polychromatic Sciences.
www.flowsols.com
15:45 - 17:30: Analysis of Flow Data
and Reporting Results
- Discussion about Fundamental
Statistics
- Introduction to MiFlowCyte: Data
Presentation Guidelines
DAY 2
9:30 - 10:00: Review of the Day 1
10:00 - 11:30: Demonstration of flow
data analysis—Cell Cycle,
Proliferation, Apoptosis, Population
comparison and multicolor assay.
11:30 - 11:45: Coffee/Tea Break
11:45 - 13:00: Troubleshooting flow
cytometry data
13:00 - 14:00: Lunch
11:30 - 11:45: Coffee/Tea Break
11:45 - 13:15: Compensation and Data
Visualization
- Importance of right Compensation and
how to perform it.
- Visualizing Data and Identifying
Populations
13:15 - 14:00: Lunch
14:00 – 15:30: Basics of Flow Data
Analysis and Presentation—What to
do and what not to do?
15:30 – 15:45: Coffee/Tea break
14:00 - 15:30: Hands on Data
Analysis and Discussion
15:30 – 15:45: Coffee/Tea break
15:45 – 16:45: Data analysis and
Discussion (continued)
16:45 - 17:30: Test and Feedback and
Valedictory
Participants should bring their own
laptop and data files (.fcs files) for
analysis and discussion. Data files
will also be provided by us.