1. Healthcare

Hormone and
Metabolie Research
Editors-in-Chief:
R. Levine, Duarte
E. F. Pfeiffer, Ulm
Assistant-Editors:
R. J. Mahler, Duarte
R. Ziegler, Ulm
Volume 6/1974
306 Figures
in 327 Single figures
and210Tables
Georg Thieme
Verlag
Stuttgart
III
T A B L E OF CONTENTS
Melanie, F.
Prohormones in Tissues and in Circulation . . .
1
Miyai, K., M. Azukizawa
and Y. K u m a h a r a
Bioassay and Immunoassay Measurements of
Serum Thyrotropin in Primary Hypothythyroidism Before and After the Administration of
Thyrotropin Releasing Hormone
9
Moody, A . J . , M . A . Stanlf, M. Stan, J. G l i e m a n n
A Simple Free Fat Cell Bioassay for Insulin . . .
Serrano-Rios,
M., F G . H a w k i n s , F. Escobar,
J.M. M a t o , L . L a r r o d e r a , M. de O y a and J . L .
Rodriguez-Minon
Insulin Secretion in Addison's Disease: Effect
of Hydrocortisone Treatment
Lücke, C., B . Höffken, H J . M i t z k a t and I.
Trau tschold
Effect of 2-Deoxy-D-Glucose and Mannoheptulose on the Insulin Response to Pancreozymin
in Rabbits
12
17
22
Lisch, H.-J., S. Sailer, F. Sandhofen
R.
Tschikof
and H.
Braunsteiner
The Action of Insulin and Glucose on Lipolysis
in Isolated Human Fat Cells
25
Werner, S. and H Low
Inhibitory Effects of Calcitonin on Lipolysis
and ^Calcium Accumulation in Rat Adipose
Tissue in Vivo
Goebell, H
The Effect of Ethionine on the Energy-Producing Metabolism in the Rat Pancreas
H o w e l l , S . L . , J.C. Edwards
and W. M o n t a g u e
Regulation of Adenylate Cyclase and CyclicAMP Dependent Protein Kinase Activities in
A -Cell Rieh Guinea-Pig Islets of Langerhans .
2
R o l d a n , A . G . , E J . del Castillo and V.G. Foglia
Effect of Cyclic AMP on Ketogenesis in Liver
Homogenates from Pancreatectomized-Hypophysectomized Dogs
R o l l e r i , E . , R . M a l v a n o , C. Gandolfi and U.
Rosa
Effect of Irreversible Adsorbents on the Dissociation of the Antigen-Antibody Complexes
.
Gautvik,
K.M. and A . H . Tashjian, Jr.
Human Medullary Thyroid Carcinoma: Control
of Calcitonin Secretion In Vivo and in Tissue
Culture
70
Boonayathap,
U. and S.F. M a r o t t a
Renal Handling of Cortisol in Dogs
74
Gennser,
G., P. L i e d h o l m and J. T h o r e i l
Pituitary Responses to Continuous Administration of LRH in Human Males with Oligozoospermia
79
Eshet, R . , Z. L a r o n , M. B r o w n and R . A r n o n
Immunological Behaviour of HGH from Plasma
of Patients with Familial Dwarfism and High
IR-HGH in a Radioimmunoassay System Using
the Cross-Reaction between HGH and HCS . . .
80
K e r p e l - F r o n i u s , E . , G. Gäcs and Ch. H e r v e l
Pituitary Function in Malnutrition
82
Legros, J.J. and P.
Lefebvre
Lack of Effect of Human Neurophysin I on the
Metabolism of Rat Adipose Tissue
83
30
Schleyer, M., K L . F e h m , K . H Voigt, J.D. Faulhaber and E.F. Pfeiffer
Studies on the Pituitary "Fettstoffwechselhormon"
36
Das, I. and D . A . Hems
Glycogen Synthetase and Phosphorylase Activities in Different Tissues of Genetically Obese
Mice
GunZaga, J., Th. Lauffenburger,
C h a r l o t t e Lentner, M . A . Dambacher,
H.G. Haas and H F l e i s c h ,
A . J . Olah
Diphosphonate Treatment of Paget's Disease of
Bone
62
40
44
49
53
57
Renauld,
A . , R . C . Sverdlik and L . L .
Andrade
Effect of Thyroidectomy upon the Insulin Tolerance Test
84
Bajaj, J.S. and J.
Vallance-Owen
Fenfluramine and Glucose Uptake by Muscle . 85
F r a y n , K . N . , A n m a r i e Hedges and Marilyn J.
Kirby
Stimulation by Fenfluramine of Glucose Uptake
into Skeletal Muscle In Vitro
86
Bercovitch,
L and J . A .
Moorhouse
Effect of Posture on Plasma Cortisol Level . . .
87
R a h e j a , K . L . and E.F. Reber
Relative Ineffectiveness of Exogenous as Compared with Endogenous Testosterone in Combating Hypercholesterolemia
88
Sutherland,
D.J.B, and R . L . S i n g h a i
Stimulation of Prostatic Adenyl Cyclase by Dihydrotestosterone
89
L e r n e , C.E., B . L . Wajchenberg,
M.O.R.
Leite
and A . B o r e l l i
The Dissociation of Urinary Sodium and Calcium Excretions
90
Rayssiguier,
Y. and P. L a r v o r
Parathyroid Response to Hypocalcemia in Magnesium Deficient Rat
91
IV
S u l m a n , G. and E l i Tal
Treatment of Functional Hypothyroidism by
Oral TRF Monitored by Daily Urinary Thyroxine and Histamine Assay
92
G h i o n e , S. M. Pellegrini,
G. Buzzigoli,
A . Carpi,
C. Valori and L . D o n a t o
Plasma and Urinary Catecholamine Levels and
Thyroid Activity in Relation to Cardiovascular
Changes in Hyper- and Hypothyroids
93
de Reviers, M. and M.P. Dubois
Binding of Synthetic-LHRF Antibodies in the
Median Eminence of the Cockerel
94
Adkofer,
F., U. Armbrecht
and H
Schleusener,
with technical assistance f r o m C.
Brammeier
and M M . Schürnbrand
Plasma Lecithin: Cholesterol Acyltransferase
Activity in Hypo- and Hyperthyroidism . . . .
142
G o r i n , E . and H M .
Goodman
Protein Kinase in Adipose Tissue: Effect of
Hypophysectomy
146
Elkeles,
R.S.
The Role of Heparin in Lipoprotein Lipase
Activity
151
Boonayathap,
U. and S.F. M a r o t t a
Renal Handling of Cortisol in Dogs. II. Effect
of ACTH on Stop Flow Pattern
154
t
K a l k , W.J., A . I . Vinik, S. B a n k , P. K e l l e r a n d
W.P. U. Jackson
Selective Loss of Beta Cell Response to Glucose in Chronic Pancreatitis
95
Bruckdorfer,
K . R . , S.S. Kang, L H K h a n , A . R .
B o u r n e and J. Y u d k i n
Diurnal Changes in the Concentrations of Plasma Lipids, Insulin and Corticosterone in Rats
fed Diets Containing Various Carbohydrates .
99
Tjälve, H , D . Popov and P. Slanina
Effect of the Local Anesthetics Procaine and
Lidocaine on Insulin Secretion from Rabbit
Pancreas Pieces
106
Loubatieres,
A . L . , M M . Loubatieres-Mariani,
R . A l r i c , G. Ribes and H Agot
Studies of the Action of Neutral Red on Glycemia and on Insulin and Glucacon Secretion in
the Dog
111
Aynsley-Green,
A . and K G . M M . A l b e r t i
In Vivo Stimulation of Insulin Secretion by
Guanidine Derivatives in the Rat
H o w l a n d , B . E . and E J .
Zebrowski
Serum and Pituitary Gonadotropin Levels in
Alloxan-Diabetic Rats
Roos, P , J M . M a r t i n , S. Westman-Naeser
and
C. Hellerström
Immunoreactive Growth Hormone Levels in
Mice with the Obese-Hyperglycemic Syndrome
(Genotype obob)
115
121
f
O D o r , R . K . , F E . Newsome,
C O . Parkes and
D.H.
Copp
Peptides Obtained from Porcine Calcitonin
After Degradation by a Human Plasma Fraction
161
Berger, W., M. Stahl, E. O h n h a u s and H
Gaschke
Pancreatic Glucagon, Plasma Insulin and Blood
Glucose Response to Arginine Infusion in Nondiabetic Subjects Following Biguanide Pretreatment
165
Davis, B . and N . R . Lazarus
Effect of Glucagon, Diazoxide, Phentolamine
on Islet Adenylate Cyclase
166
Strohfeldt,
P , H Kettl and K.F.
Weinges
Perfusion of the Isolated Rat Hindlimb with
a Synthetic Medium
167
M e i n h o l d , H. and K. W. Wenzel
Radioimmunoassay of Thyroxine in Unextracted Serum
169
125
R a b k i n , R . , Margaret S w a n n , D J . Shapiro and
L.
Isaacson
Effect of Growth Hormone on Sodium Transport and Osmotic Water Flow AcrossToadSkin . 129
Pokroy, N . , S. Epstein, Salle H e n d r i c k s and B .
Pimstone
Thyrotrophin Response to Intravenous Thyrotrophin-Releasing Hormone in Patients with
Hepatic and Renal Disease
Siegmund,
P , A . Tüllmann and M. H o l k e with
the technical
collaboration
of P. Ott and L .
Uher
The Cyclic AMP-Mediated Action of Epinephrine and the Activity of Carbonic Anhydrase in
Avian Erythrocytes
158
132
Renauld, A . , R . C . Sverdlik a n d L . L .
Andrade
Effects of Hypothyroidism on Serum Immunoreactive Insulin, Free Fatty Acids and Blood Sugar
in the Dog as Tested for Oral Glucose Tolerance.
Corrective Effects to Thyroxine Therapy
137
Nieschlag,
E . , T Walk and A . E .
Schindler
Dehydroepiandrosterone (DHA) and DHASulfate during Pregnancy in Maternal Blood. .
170
Mauss, J. and G. Borsch
Clomiphene Citrate Stimulation in Male Suband Infertility Monitored by Simple Immunochemical Assays for FSH and LH in Unconcentrated Urine
171
N e r u p , J., O. Ortved Andersen,
G.
Bendixen,
J. Egeberg, R . Gunnarsson,
H. K r o m a n n and
J.E.
Poulsen
Glucose Intolerance and Islet Damage in Mice
Immunized with Homologous Endocrine
Pancreas — A Preliminary Communication . .
173
V
K u m a r , D . , S . D . M e h t a l i a and L . V. Miller
Antigenicity of Monocomponent Porcine
Insulin in Rabbits
175
Pento, J.T., A . Kagan and S M G l i c h
Influence of Altered States of Calcium Homeostasis on Insulin Secretion in Rats and Rabbits 177
Somogyi,
J., I. Vincze, F. Willig and F . K
Schmidt
The Subcellular Distribution of ^-Glibenclamide in Different Tissues of Rat
181
Tchobroutsky,
G., M.-E. Lenormand,
G.
M i c h e l and R . Assan
Lack of Post Prandial Exercise-Induced Growth
Hormone Secretion in Normoglycemic InsulinTreated Diabetic Men
184
Clemens, J . A . , C J . Shaar, E . B . Smalstig
and
C.
Matsumoto
Effects of Some Psychoactive Agents on Prolactin Secretion in Rats of Different Endocrine States
Gilbert, C . K and D J . G a l t o n
The Effect of Catecholamines and Fasting on
Cyclic-AMP and Release of Glycerol from
Human Adipose Tissue
229
Cox, B . D . , A . R . Clarkson, M J . Whichelow and
P. R u t l a n d
Effect of Adrenaline on Plasma Vitamin C
Levels in Normal Subjects
234
Ward, W.F., K.M. K l i t g a a r d and R . C Meade
Insulin Degradation by Fat Cell Ghosts . . . .
238
H e r r m a n n , J, H J . Rusche, H J . Kröll, P. Häger and K L . Krüskemper
Free Triiodothyronine (T )- and Thyroxine
(T )-Serum Levels in Old Age
239
Galloway,
D . G . and J.
Pelletier
Influence of Age on the Pituitary Response of
Male Lambs to Synthetic LH-RH Injection . .
240
Wickramasinghe,
R.K
Effect of 11-Deoxycorticosterone on Cholesterol Sidechain Cleavage by Adrenal Cortex
Mitochondria
241
Jerums, G. and D J . G a l t o n
Two Types of Insulin Receptors Mediating
Opposite Effects on Cyclic-AMP in Fat Cells
of the Rat
242
G o z a r i u , L . , K. Forster, J . D . F a u l h a b e r , H.
M i n n e and R . Ziegler
Parathyroid Hormone and Calcitonin: Influences upon Lipolysis of Human Adipose
Tissue
243
T i e n g o , A . , D . Fedele, P. Frasson, M.
Muggeo
and G. Crepaldi
Ethanol Effect on Glucagon Secretion in the
Pig
245
3
187
P a v l o v i c - H o u r n a c , M. and D .
Delbauffe
In Vivo Incorporation of Labelled Amino Acid
into Different Protein Fractions of the Rat
Thyroid Gland
196
202
Klenerova,
V. and S. Kynie
The Activity of Adenylate Cyclase, Phosphodiesterase and Protein Kinase in the Adenohypophyses of Rats Treated by Chronic Administration of Estrogenes and Acute Application of Thyroxine
208
Kley, KK, E. Nieschlag,
F. B i d l i n g m a i e r and
K L . Krüskemper
Possible Age-Dependent Influence of Estrogens on the Binding of Testosterone in Plasma
of Adult Men
213
Sladek,
CD.
Gluconeogenesis and Hepatic Glycogen Formation in Relation to the Rat Estrous Cycle .
217
Adolfsson,
S., S. Boström, M. F a h l e n , A .
Hjalmarson,
B . E . Hustvedt
and R .
Johansson
Enzyme Activities in Muscle and Liver After
Destruction of the Ventromedial Hypothalamus Area and Administration of Insulin
226
4
V H e r m i t e , M . , C Robyn,
J. Golstein,
G.
Rothenbuchner,
J. B i r k , U. Loos, M. Bonnyns
and L .
Vanhaelst
Prolactin and Thyrotropin in Thyroid Diseases:
Lack of Evidence for a Physiological Role of
Thyrotropin-Releasing Hormone in the Regulation of Prolactin Secretion
190
Busnardo,
B . , M.E. Girelli, L . V a r o t t o and A .
Giacobbi
Computer Analysis of Early Thyroxine Distribution in Thyrotoxicosis and Hypothyroidism
Macho, L .
The Response of Adipose Tissue to Lipid
Mobilizing Hormones in Postnatally Underand Overnourished Rats
Kissebah, A . K , N. Vydelingum,
B . R . Tulloch,
H H o p e - G i l l and T R . Fräser
The Role of Calcium in Insulin Action.
I. Purification and properties of enzymes regulating lipolysis in human adipose tissue: effects
of cyclic-AMP and calcium ions
247
G o b e r n a , R . , J. T a m a r i t Jr., J. Osorio, R . Fussgänger, J. T a m a r i t and E . F . Pfeiffer
Action of B-Hydroxy Butyrate, Acetoacetate
and Palmitate on the Insulin Release in the
Perfused Isolated Rat Pancreas
256
Rehfeld, J.F. and J. Iversen
Secretion of Immunoreactive Gastrin from
the Isolated, Perfused Canine Pancreas . . . .
222
260
VI
Alemany,
M. and E . Herr era
Effects of Suckling and Food Deprivation on
the Acitivity of Citrate Enzymes in the Liver
of the Rat
264
H o w e l l , S . L . , C. Hellerström and M.
Tyhurst
Intracellular Transport and Storage of Newly
Synthesised Proteins in the Guinea Pig Pancreatic A Cell
267
Z e r m a t t e n , A . and J.-P. F e l b e r
Sensitivity to Glucose of an Intestinal Factor
Stimulating Insulin Release
Vargas, L . , M. Bronfman
and M.E. K a w a d a
Stress, Insulin Antagonist and Transient Diabetes Mellitus in the Rat
Tarachand,
U. and J. E a p e n
Effect of Streptozotocin-Diabetes on In Vivo
Protein Synthesis by Placenta and Liver of
Mice
Schönborn, J., W. Poser U. Panten and A .
Hasselblatt
Effect of Hypoglycemic Sulfonylureas on
Hepatic Fructose Metabolism in the Rat . .
272
Werner, S., K. Hall and H Low
Similar Effects of Calcitonin, Insulin and Somatomedin A on Lipolysis and Uptake of Calcium and Glucose in Rat Adipose Tissue In
Vitro
319
275
Gleispach,
H.
The Urinary Steroid Excretion in Girls during
Puberty
325
280
M a r o t t a , S . F . , D . M . L a n u z a and L . G . Hiles
Diurnal Variations in Plasma Corticosterone
and Cations of Male Rats on Two Lighting
Schedules
329
284
Bates, R . F . L . and J.P. B a r l e t
The Preventive Effect of Porcine Calcitonin
given by Mouth on Restraint-Induced Gastric
Ulcer in Rats
332
Kappus,
H and H M . Bolt
Irreversible Binding of Ethynylestradiol Metabolites to Protein: Lack of Methylation by Sadenosyl-Methionine
333
Kato, H , T I t o , Y. Kido and T
Torigoe
Preparation of 1-Labeled Estrone-Tri Tyrosine Methyl Ester
334
f
Rado, J.P.y L . Borbely,
L . Szende
J. Fischer
and J. T a k o
Investigation of the Diuretic Effect of Glibenclamide in Healthy Subjects and in Patients
with Pituitary and Nephrogenic Diabetes
Insipidus
289
t
M a r s c h n e r , L , P. B o t t e r m a n n , F. E r h a r d t , R .
L i n k e , G. Löffler, V. Maier, P.
Schwandt,
W. Vogt and P C . Scriba
Group Experiments on the Radioimmunological Insulin Determination
S u t t e r - D u b , M.Th.
R . Leclercq,
B . Ch.J.
Sutter and R .
Jacquot
Plasma Glucose, Progesterone and Immunoreactive Insulin Levels in the Lactating Rat .
Lilienfeld-Toal,
H v., R . - D . Hesch, M. Hüfner
and Chr.
Mclntosh
Excretion of Cyclic 3', 5'-Adenosine Monophosphate in Renal Insufflciency and Primary Hyperparathyroidism after Stimulation with Parathyroid Hormone
314
125
293
Rastogi, G.K., J. C h a k r a b o r t i and M.K. S i n h a
Serum Gonadotropins (LH and FSH) and their
Response to Synthetic LHRH in Diabetic Men
with and without Impotence
335
297
Mohnfeld,
G., J. Mauss and G. Borsch
Synthetic LH-RH Stimulation Monitored by
Immunochemical Assay for Urinary FSH and
LH
f
336
Voigt, K . H . , H G . D a h l e n , H L . F e h m , J. B i r k ,
K. Schröder, H P . G . Schneider,
G. Rothenbuchner and E.F.
Pfeiffer
Simultaneous Stimulation Test for the Anterior
Pituitary Hormones
337
Hervas, F. and G. M o r r e a l e de Escobar
A Rapid Procedure for the Radioimmunoassay of Rat Growth Hormone
300
Kowadlo
Silbergeld,
A . and Z. L a r o n
Lipolytic Effects of Sheep (3-Lipotropin in Rat
Adipose Tissue: Interaction with Theophylline
and Dihydroergotamine
303
Pfeiffer,
E . F , Ch. T h u m and A . H
The Artificial Beta Cell
306
I m u r a , H., Y. N a k a i , Y. Kato, Y.
Yoshimoto
and K. M o r i d e r a
Propranolol-Insulin Stimulation Test in the
Diagnosis of Growth Hormone Deficiency . .
343
B e r l e , P . , E. Finsterwalder
and M.
Apostolakis
Comparative Studies on the Effect of Human
Growth Hormone, Human Prolactin and
Human Placental Lactogen on Lipid Metabolism
347
Berthezene,
F., B . Chavrier and R . M o r n e x
Increase in the Amount of Iodide Released
from the Thyroid after Perchlorate or Carbimazole Administration
Jortay, A . M . , F . R . L . C a n t r a i n e and J.E. D u mont
Iodide Trapping by Thyroid Slices in Vitro . .
309
Clemens
339
VII
Ogundipe,
0.0. and A . Bray
The Influence of Diet and Fat Cell Size on
Glucose Metabolism, Lipogenesis and Lipolys351
is in the Rat
Kissebah, A . H . , B . R . T u l l o c h , N .
Vydelingum,
H Hope-Gill,
P. Clarke and T . R . Fräser
The Role of Calcium in Insulin Action . . . .
357
Werner, S, and H Low
Adenylate Cyclase in Rat Adipocyte Plasma
Membranes after Adrenalectomy and Administration of Cortisone Acetate
365
G o l d m a n , J.K. and L . L . Bernardis
Metabolism of Glucose, Fructose and Pyruvate
in Tissues of Weanling Rats with Hypothalamus
Obesity
370
F r a n c o i s e R a b a i n and L . Picon
Effect of Insulin on the Materno-Fetal Transfer of Glucose in the Rat
A n a A r a n d a and E. Herr era
The Effect of Food Deprivation on In Vivo
Gluconeogenesis in the Suckling Rat
Gaschke,
H , A . Denes, J. Girard, F. Collard
and W. Berger
Circadian Variations of Carbohydrate Tolerance in Maturity Onset Diabetics Treated with
Sulfonylureas
376
381
S u v a r n a l a t h a , M. and H.B. Devaraj S a r k a r
Effect of Steroids on Hemicastration Induced Compensatory Hypertrophy of the
Testis in the Frog, Rana hexadactyla
(Lesson)
417
Debry, G., P. D r o u i n and L . Mejean
Influence of Triglyceridemia Level on Glucose
Tolerance and Insulinemia in Subjects with
Type IV Hyperlipoproteinemia
421
Bittner, R . , H G . Beger and E . Kraas
Osciilation in Insulin Response to Glucose
Stimulation
423
Sundby, F . , J. Markussen
and W. D a n h o
Camel Glucagon: Isolation, Crystallization
and Amino Acid Composition
425
L a u b e , H , R . D . Fußgänger and E.F.
Pfeiffer
Paradoxical Glucagon Release in Obese Hyperglycemic Mice
426
Velasco, C, W. O p p e r m a n n , N. Marine and R .
Camerini-Davalos
Effect of Genetic Diabetes on Kidney Glucosyltransferase
427
Sutton, J. and L . Lazarus
Effect of Adrenergic Blocking Agents on Growth
Hormone Responses to Physical Exercise
. . 428
3
8
6
Rado, J.P., L . Vegh and I r e n e
Sawinsky
Interference of Triamterene with Cortisol
Determination in Urine
429
Yang, M.M.P.
and S . H Kok
Effect of a Single Dose of Progesterone on
Blood Glucose in Diabetic Rats and on Insulin
Sensitivity in Normal Rats
430
Bolt, W.H, H. Kappus and H M . Bolt
Ring A Oxidation of 17a-Ethynylestradiol in
Man
432
Steiner, H , M. Z a n i s i and L . M a r t i n i
Antiovulatory Activity of the Thyrotropin Releasing Hormone in the Rat
432
Cabello, G. and M.C. M i c h e l
Plasmatic Hormonal Iodine in Healthy and
Diarrheic Calves
434
Becker, F . O . , F. B u c h a n a n and M. Rasho
Parathyroid Hormone Degradation Studies in
Maternal and Fetal Rat Tissues by Radioimmunoassay
Sawhney,
R . C . and G.K.
Rastogi
Estimation of TBG by a Competitive LigandBinding Assay
435
Leicht, E , G. B i r o and K.F.
Weinges
Inhibition of Releasing-Hormone-Induced
Secretion of TSH and LH by Calcitonin . . . .
Voigt, K.H., H G . D a h l e n , H L . F e h m , L . B i r k ,
K. Schröder, H P . G . Schneider,
G.
Rothenbuchner and E.F.
Pfeiffer
Simultaneous Stimulation Test for the Anterior
Pituitary Hormones
436
Massi-Benedetti,
F., A . Falorni, A . Luyckx and
P.
Lefebvre
Inhibition of Glucagon Secretion in the Human Newborn by Simultaneous Administration of Glucose and Insulin
Vinik, A . L and A .
Hardcastle
Structure Antigenicity Relationships of Glucagon and Related Peptides
Renauld,
A . , L . L . Andrade,
R . C . Sverdlik and
R.R.
Rodriguez
Serum Insulin Response to Glucose Infusion
in Hyperthyroid Dogs
Story, J . A . and D . R .
Griffith
Effect of Exercise on Thyroid Hormone
Secretion Rate in Aging Rats
B o r r e i l , J and S. B o r r e l l
Alterations of Adrenal Corticosteroids and
Catecholamines Düring Arnino-Glutethimide
Feeding
4
0
3
F i n k , G., J.C. Cresto, R . A . G u t m a n , R . I .
L a v i n e , A . H . Rubenstein
and L . Recani
Plasma Proinsulin-Like Material in Insulin Treated Diabetics
439
VIII
R a n c o n , F . , M. L a b u r t h e , G. Rosselin and P.
Freychet
Untractable Hypoglycemia in an Infant: Studies
on Pancreas Insulin and Glucagon
443
A n d r e a n i , D . , M. I a v i c o l i , G. T a m b u r r a n o and
G. M e n z i n g e r
Comparative Trials with Monocomponent (MC)
and Monospecies (MS) Pork Insulins in the
Treatment of Diabetes mellitus. Influence on
Antibody Levels, on Insulin Requirement and
on Some Complications
447
G o l d m a n J.K. and L . L .
Bernardis
Persistent Adipose Tissue Insulin Responsiveness Düring Fasting of Weanling Rats with
Hypothalamus Obesity
t
H o p e - G i l l H , N. Vydelingum,
A.H
Kissebah,
B . R . T u l l o c h and T R . Fräser
Simulation and Enhancement of the Adipose
Tissue Insulin Response by Procaine Hydrochloride: Evidence for a Role of Calcium in
Insulin Action
Rosak, C., E. Haupt, J. Beyer and K. Schaffung
Investigations on the Antilipolytic Activity of
Sulfonylureas in Man with Indications of
Limit Dosages Concerning their Insulin-Secreting Properties
Lambert,
A . E . , J.C. H e n q u i n and P. Malvaux
Cationic Environment and Dynamics of Insulin
Secretion. IV. Effect of Ouabain
454
457
464
470
Rothenbuchner,
G., D A . Koutras,
S. Raptis,
J. B i r k , U. Loos, G. Rigopoulos
and B. Malamos
The Effect of Thyrotrophin-Releasing Hormone
on Serum TSH, T , and T Levels in Endemie
and Sporadic Nontoxic Goitre
501
4
3
Golstein,
J., E . Van Cauter, R . Leclercq,
G.
Copinschi
and L .
Vanhaelst
Monthly Variations of Serum Levels of Thyrotropin, Growth Hormone in Cortisol in Man .
506
D a h l e n , H G . , E. Keller and H.P.G.
Schneider
Linear Dose Dependent LH Release Following
Intranasally Sprayed LRH
510
Baier, H , B i r o and K.F. Wernges
Serum Levels of FSH, LH and Testosterone in
Human Males
514
B a r l e t , J.P.: The Influence of Porcine Calcitonin Given Intragastrally on Restraint-Induced Gastric Ulcers in Pigs
.
517
A n t o n i o , P . , M. G a b a l d o n , T Lacomba and
A . Juan
Effect of the Antiestrogen Navoxidine on the
Occurrence of Estrogen-Dependent Renal Tumors in Hamster
522
Clarke, P , A . Kissebah, N. Vydelingum,
H
Hope-Gill,
B . T u l l o c h and R . Fräser
Regulation of Glycogenolysis through Changes
in Intracellular Calcium
525
Vinik, A . L and S. Jessop
Red Blood Cell Potassium and Insulin Release
526
Schimmel,
R . J . and D . G r a h a m
Inhibition by Diphenylhydantion of the Diabetogenic Action of Streptozotocin
475
O h n e d a , A . , M. Sato, K. M a t s u d a , H I t a b a s h i ,
K. H o r i g o m e , M. C h i b a and S.
Yamagata
Suppression of Pancreatic Glucagon Secretion
by Tolbutamide in Dogs
Dolais-Kitabgi,
Jacqueline,
M. L a b u r t h e and G.
Rosselin
Immunological Specificity of the ß Subunit of
the Human Chorionic Gonadotropin (HCG)
527
478
K r a l , J . G . : The Effects of Different Methods
of Sacrifice on Lipid and Carbohydrate Metabolism in the Rat
Rastogi, G.K., R . C . Sawhney,
N.C. P a n d a and
B. B.
Tripathy
Thyroid Hormone Levels in Adult Protein Calorie Malnutrition (PCM)
528
483
Boninsegna,
A . , G. Federspil
and C. D e P a l o
The Effect of Muscular Exercise on Free
Fatty Acids, Acetoacetate and 3-Hydroxybutyrate Blood Levels
488
Tarachand,
U. and J. E a p e n
A Comparative Study on In Vivo Protein Synthesis by Liver of Pregnant and Non-pregnant
Mice
491
Schimpff,
R . M . , M. D o n n a d i e u , M.
Gourmelen
and F. Girard
The Effects of hGH Treatment on Somatomedin Levels in the Serum (as determined by
S uptake into cartilage)
494
3 5
Vinik, A . L , W.J. K a l k , H M c L a r e n and M. Paul
Impaired Prolactin Response to Synthetic Thyrotropin-Releasing Hormone after a 36 Hour Fast . 499
Thomas, J . A . and M.
Manandhar
Effect of Prolactin and/or Testosterone on
Cyclic AMP in the Rat Prostate Gland . . . . 529
y
'
/
Rado, J.P., T Simon, E. Juhos, J. T a k o and
O. Nagy
Interference of Psychotropic Drugs with
Cortisol Determinations
530
Sönka, J., J. Kopeckä, A . Pavlovd, A . Z b i r k o vä and J. Stas
Effects of Diet and Exercise on Catecholamine
Excretion
532
Meyer, Jr., R . A . and Martha H Meyer
Increased Liver Inorganic Phosphate Following
Thyrocalcitonin Administration to Rats . . . . 533
Offermann,
G. and F. D i t t m a r
A Direct Protein-Binding Assay for 25-Hydroxycalciferol
534
IX
AUTHOR'S INDEX
A
Adlkofer, F. 142
Adolfsson, S. 222
Agot, H. 111
Alberti, K.G.M.M. 115
Alemany, M . 264
Alric, R. 111
Andrade, L . L . 84, 137, 400
Andreani, D. 447
Antonio, P. 522
Apostolakis, M. 347
Aranda, A N A . 381
Armbrecht, U. 142
Arnon, R. 80
Assan, R. 184
Aynsley-Green., A . 115
Azukizawa, M . 9
B
Baier, H . 514
Bajaj, J.S. 85
Bank, S. 95
Barlet, J.P. 332, 517
Bates, R.F.L. 332
Becker, F.O. 407
Beger, H.G. 423
Bendixen, G. 173
Bercovitch, L. 87
Berger, W. 165, 386
Berle, P. 347
Bernardis, L . L . 370, 454
Berthezene, F. 306
Beyer, J. 464
Bidlingmaier, F. 213
Birk, L 190, 337,436,501
Biro, G. 410, 514
Bittner, R. 423
Borsch, G. 171, 336
Bolt, H.M. 333
Boninsegna, A . 488
Bonnyns, M. 190
Boonayathap, U . 74, 154
Borbely, L. 289
Borreil, J. 414
Borreil, S. 414
Borelli, A . 90
Boström, S. 222
Bottermann, P. 293
Bourne, A.R. 99
Braunsteiner, H. 25
Bray, A. 351
Bronfman, M . 275
Brown, M . 80
Bruckdorfer, K.R. 99
Buchanan, F. 407
Busnardo, B. 202
Buzzigoli, G. 93
C
Cabello, G. 434
Camerini-Davalos, R.A. 427
Cantraine, F.R.L. 309
Carpi, A . 93
Castülo, Del. E J . 53
Cauter, E. van 506
Charkaborti, J. 335
Chavier, Bernadette 306
Chiba, M. 478
Qakson, A.R. 234
Clarke, P. 357, 525
Clemens, A.H. 339
Clemens, J.A. 187
Collard, F. 386
Copinschi, G. 506
Copp, D.H. 161
Cox, B.D. 234
Crepaldi, G. 245
Cresto, J.C. 439
D
Dahlen, H.G. 436, 510
Dahlen, H.L. 337
Dambacher, M.A. 62
Danho, W. 425
Das, I. 40
Davis, B. 166
Debry, G. 421
Delbauffe, Daniele 196
Denes, A . 386
De Pala, C. 488
Devaraj-Sarkar, H.B. 417
Dittmar, F. 534
Dolais-Kitabgi, Jacqueline 527
Donato, L. 93
Donnadieu, M. 494
Drouin, P. 421
Dubois, M.P. 94
Dumot, J.E. 309
E
Eapen, J. 280, 491
Edwards, J.C. 49
Egeberg, J. 173
Elkeles, R.S. 151
Epstein, S. 132
Erhardt, F. 293
Escobar, F. 17
Eshet, R. 80
Gennser, G. 79
Ghione, S. 93
Giacobbi, A . 202
Gilbert, C.H. 229
Girard, F., 494
Girard, J., 386
Girelli, M.E. 202
Gleispach, H., 325
Glick, S.M. 177
Goberna, R. 256
Goebell, H. 44
Göschke, H. 386
Goldman, J.K. 370, 454
Golstein, J. 190, 506
Goodman, H.M. 146
Gorin, E. 146
Goschke, H. 165
Gourmelen, M. 494
Gozariu, L . 243
Graham, D. 475
Griffith, D.R. 403
Guncaga, J. 62
Gunnarsson, R. 173
Gutman, R.A. 439
H
Haas, H.G. 62
Hau, K. 319
Hardcastle, A . 396
Hasselblatt, A . 284
Haupt, E. 464
Hawkins, F.G. 17
Hedges, Annmarie 86
Hellerström, C. 125, 267
Hems, D.A. 40
Hendricks, Salie 132
Henquin, J.C. 470
Hermite, M.L. 190
Herrera, E. 264, 381
Hervas, F. 300
Hervei, Ch. 82
Hesch, R.D. 314
Hialmarson, A . 222
Hiles, L.G. 329
Höffken, B. 21
Holke, M. 158
Hope-Gill, H. 247, 357, 457, 525
Horigome, K . 478
Howell, S.L. 49, 267
Howland, B.E. 121
Hüfner, M . 314
Hustvedt, B.E. 222
Hynie, S. 208
F
Fahlen, M. 222
Falorni, A. 392
Faulhaber, J.D. 36/243
Fedele, D. 245
Federspil, G. 488
Fehm, H.L. 36, 337/436
Felber, J.R. 272
Fink, G. 439
Finsterwalder, E. 347
Fischer, J. 289
Fleisch, H . 62
Foglia, V.G. 53
Forster, K. 243
Fräser, R. 525
Fräser, T.R. 247, 357, 457
Frasson, P. 245
Frayn, K . N . 86
Freychet, P. 443
Fussgänger, R. 256
Fussgänger, R.D. 426
I
Iavicoli, M. 447
Imura, H. 343
Isaacson, L . 129
Itabashi, H. 478
Ito, T. 334
Wersen, J. 260
G
Gabaldon, M. 522
Gäcs, G. 82
Galloway, D.G. 240
Galton, D.J. 229, 242
Gandolfi, C. 57
Gautvik, K.M. 70
J
Jackson, W.P.U. 95
Jacquot, R. 297
Jerums, G. 242
Jessop, S. 526
Johansson, R. 222
Jortay, A . M . 309
X
Juan, A . 522
Juhos, E. 530
K
Kagan, A . 177
Kalk, W.J. 95, 499
Kang, S.S. 99
Kato, H. 334
Kato, Y . 343
Kappus, H. 333
Kawada, M.E. 275
Keller, E. 510
Keller, P. 95
Kerpel-Fronius, E. 82
Kettl, H. 167
Khan, L H . 99
Kido, Y . 334
Kirby, Marilyn, J. 86
Kissebah, A . 525
Kissebah, A . H . 247, 357, 457
Klenerova, V . 208
Kley, H.K. 213
Klitgaard, H.M. 238
Kok, S.H. 430
Kopecka, J. 532
Koutras, D.A. 501
Kowadlo Silbergeld, A . 303
Kraas, E. 423
Kral, J.G. 483
Kromann, H. 173
Krüskemper, H.L. 213
Kumahara, Y. 9
Kumar, D. 175
L
Laburthe, M . 443, 527
Lacomba, T. 522
Lambert, A.E. 470
Lanuza, D.M. 329
Laren, H . , Mc. 499
Laron, Z. 80, 303
Larrodera, L. 17
Larvor, P. 91
Laube, H. 426
Lauffenburger, Th. 62
Lawine, R . L . 439
Lazarus, L. 428
Lazarus, N.R. 166
Leclercq, R. 297, 506
Lefebvre, P. 83/392
Legros, J.J. 83
Leicht, E. 410
Leite, M.O.R. 90
Lerne, C E . 90
Lenormand, Marie-Emmanuelle 184
Lentner, Charlotte 62
Liedholm, P. 79
v. Lilienfeld-Toal, H. 314
Linke, R. 293
Lisch, H.-J. 25
Löffler, G. 293
Low, H . 30/319/365
Loos, U . 190, 501
Loubatieres, A . L . 111
Loubatieres-Mariani, M.M. 111
Lücke, C. 21
Luyckx, A . 392
M
Macho, L. 226
Maier, V. 293
Malmos, B. 501
Maltarello, C , Miss 447
Malvano, R. 57
Malvaux, P. 470
Manandhar, M. 529
Marine, N . 427
Markussen, J. 425
Marotta, S.F. 74, 154, 329
Marschner, I. 293
Martin, J.M. 125
Martini, L. 432
Massi-Benedetti, F. 392
Mato, J.M. 17
Matsuda, K . 478
Matsumoto, C. 187
Mauss, J. 171, 336
Mclntosh, Chr. 314
Meade, R.C. 238
Mehtalia, S.D. 175
Meinhold, H. 169
Mejean, L. 421
Melani, F. 1
Menzinger, G. 447
Meyer, Martha, H. 533
Meyer, Ralph, A . Jr. 533
Miayai, M . 9
Michel, G. 184
Michel, M.C. 434
Miller, L . V . 175
Minne, H . 243
Mitzkat, H J . 21
Mohnfeld, J. 336
Montague, W. 49
Moody, A . J . 12
Moorhouse, J.A. 87
Moridera, K . 343
Mornex, R. 306
Morreale, de Escobar, G. 300
Muggeo, M. 245
N
Nagy, O. 530
Nakai, Y . 343
Nerup, J. 173
Newsome, F.E. 161
Nieschlag, E . 170, 213
O
O'dor, R . K . 161
Offermann, G. 534
Ogunipe, O.O. 351
Ohneda, A . 478
Ohnhaus, E. 165
Olah, A . J . 62
Oppermann, W. 427
Ortvedandersen, O. 173
Osorio, J. 256
Oya, de, M. 17
P
Panda, N.C. 528
Panten, U. 284
Parkes; C O . 161
Paul, M . 499
Paulsen, J.E. 173
Pavlova, A . 532
Pavlovig, Mira. 196
Peletier, J. 240
Pellegrini, M . 93
Pento, J.T. 177
Pfeiffer, E.F. 36, 256, 337, 339, 426,436
Picon, L. 376
Pimstone, B. 132
Pokroy, N . 132
Popov, D. 106
Poser, W. 284
R
Rabain, Francoise 376
Rabkin, R. 129
Rado, J.P. 289,429,530
Raheja, K . L . 88
Rancon, F. 443
Raptis, S. 501
Rasho, M. 407
Rastogi, G.K. 335,435,528
Rayssiguier, Y . 91
Reber, E.F. 88
Recant, L. 439
Rehfeld, J.F. 260
Renauld, A . 84, 137,400
Reviers, de. M. 94
Ribes, G. 111
Rigopoulos, G. 501
Robin, C. 190^
Rodriguez-Minon, J.L. 17
Rodriguez, R.R. 400
Roldan, A . G . 53
Rolleri, E. 57
Rosa, U. 57
Rosak, C 464
Roos, P. 125
Rosselin, G. 443,527
Rothenbuchner, G. 190, 337,436, 501
Rubenstein, A . H . 439
Rutland, P. 234
S
Sauer, S. 25
Sandhofer, F. 25
Sato, M. 478
Sawhney, R.C. 435,528
Sawinsky, I. 429
Schimpff, R.M. 494
Schimmel, R.J. 475
Schindler, A . E . 170
Schleusener, H . 192
Schleyer, M. 36
Schmidt, F.H. 181
Schneider, H.P.G. 337, 436, 510
Schöffling, K . 464
Schönborn, J. 284
Schröder, K. 337, 436
Schwandt, P. 293
Scriba, P.C. 293
Serrano-Rios, M . 17
Shaar, C J . 187
Shapiro, D.J. 129
Siegmund, P. 158
Simon, T. 530
Singhai, R.L. 89
Sinha, M.K. 335
Sladek, Celia, D. 217
Slanina, P. 106
Smalstig, E.B. 187
Somogyi, J. 181
Sonka, J. 532
Stahl, M. 165
Stan, M. 12
Stan, M.A. 12
Stas,J. 532
XI
Steiner, H. 432
Story, J.A. 403
Strohfeldt, P. 167
Sulman, G. 92
Sundby, F. 425
Sutherland, D J . B . 89
Sutter, B.Ch. 297
Sutter-Dub, M.Th. 297
Sutton, J. 428
Suvarnalatha, M . 417
Sverdük, R.C. 84,137,400
Swann, Margaret 129
Szende, L. 289
T
Takrf, J. 289,530
Tal, Eli. 92
Tamarit, J. 256
Tamarit, J. Jr. 256
Tamburrano, G. 447
Tarachand, U. 280, 491
Tashjian, A . H . J.R. 70
Tchobroutsky, G. 184
Thomas, J.A. 529
Thoreil, J. 79
Thum, Ch. 339
Tiengo, A. 245
Tjälve, H. 106
Torigoe, T. 334
Trautschold, I. 21
Tripathy, B.B. 528
Tschikof, R. 25
Tüllmann, A . 158
Tulloch, B. 525
Tulloch, B.R. 247, 357, 457
Tyhurst, M. 267
V
Vallance-Owen, J. 85
Valori, C. 93
Vanhaelst, L . 190,506
Vargas, L . 275
Varotto, L. 202
Vegh, L. 429
Velasco, C. 427
Vincze, I. 181
Vinik, A.I. 95,396, 499, 526
Vogt, W. 293
Voigt, K . H . 36, 337,436
Vydelingum, N . 247, 357,457,
W
Wajchenberg, B.L. 90
Walk, T. 170
Ward, W.F. 238
Weinges, K . F . 167,410,514
Wenzel, K.W. 169
Werner, S. 30, 319, 365
Westman-Naeser, S. 125
Whichelow, M.J. 234
Wickramasighe, R.H. 241
Willig, F . 181
Y
Yamagata, S. 478
Yang, M.M.P. 430
Yoshimoto, Y . 343
Yudkin, J. 99
Z
Zanisi, M . 432
Zbirkova, A . 532
Zebrowski, E.J. 121
Zermatten, A. 272
Ziegler, R. 243
XII
SUBJECT INDEX
A
A-cell, intracellular transport of
proteins 267
A-2-cell 49
Acetoacetate 256, 488
ACTH 1, 36, 226
effect on reabsorption of Cortisol
154
Addison's disease 17
Adenine nucleotides, pancreas 44
- , surface fluorescence 284
Adenohypophysis 208
Adenylate cyclase 208, 365, 443
- , islets of Langerhans 49,166
- , prostatic 89
Adipose Tissue 226, 357, 37G, 454
- and cells, rat
- , human 229
- , protein kinase 146
- , rat 83
Adrenal cortex, mitochondria 241
Adrenalectomized dogs, neutral red
111
Adrenalectomy 30, 365
Adrenal gland, cat 414
Adrenaline 49, 234, 357
Adrenergic blocking agents 428
Adrenocorticotropic normo ne 1, 36,
154, 226
Age dependence, binding of testosterone 213
Age, influence on pituitary 240
- , thyroid hormones 403
Albino rat 483
Allergie reactions 447
Alloxan 121
Alpha-cell, see also A-cell 392
Amiloride hydrochloride 115
Amino acid uptake, thyroid gland 196
Amino glutethimide administration
414
Anesthetics, local 106
Anterior pituitary hormones 337
- , simultaneous Stimulation test 436
Antibodies, insulin 175
Antigen-antibody complex 57
Anti-insulin serum 376
Antilipolysis 319
Antilipolytic activity 464
Antiovulatory activity 432
Antiserum heterogeneity 57
Arginine, chronic pancreatitis 95
- infusion 165
Artificial 0-cell 339
- pancreas 339
Ascorbic acid 234
Athyreosis 190
Atropine 115
Autoimmunity, endoerine pancreas
173
Autoradiography, thyroid slices 309
B
0-blockade 234
Beta-cell 392
"Big"-ACTH 1
- gastrin 1
- glucagon 1
- growth hormone 1
Biguanides 165
Bioassay, insulin 12
- , thyrotropin 9
Blood pressure, neutral red 111
C
Calcitonin 30, 319,410,517
human 161
- , lipolysis 243
- , porcine 161
ulcers 332
- , release 70
Calcium 70, 247, 319/329/365
- balance 62
- , calcitonin 30
- excretion, urinary 90
- , homeostasis 177
- , intracellular 525
- kinetics 62
Caloric intake 351
Calvarium 407
camp, see cyclic AMP
Carbimazole 306
Carbohydrate metabolism 483
Carbon dioxide 457
Carbonic anhydrase 158
Cardiovascular changes, thyroid Dysfunetions 93
Catecholamine excretion 532
Catecholamines 229, 414
- , levels in plasma and urine 93
- , release, neutral red 111
Chlorpropamide 386
Cholecystokinin, chronic pancreatitis 95
Cholesterol 403
- aeyltransferase 142
- , sidechain cleavage 241
Chronic pancreatitis 95
Circadian variations, carbohydrate tolerance 386
Citräte enzymes 264
Clomiphene ci träte, male 171
Coma, diabetic 339
Computer analysis, thyroxine distribution 202
Copper 329
Cortical cells 417
Corticosteroids 414
Corticosterone, diurnal rhythm 99
Cortisol 213,506
- , determination 429
influence of psychotropic drugs 5 30
- , plasma level 87
- , reabsorption site 154
- stop flow 154
Cortisone 365
- acetate 30
C-Peptide 1
Cross-reaction, HGH and HCS 80
Cyclic AMP 53, 70, 158, 208, 229, 247,
357, 365,457,529
- , excretion 314
- , fat cells of the rat 242
D
Dehydroepiandrosterone, pregnancy 170
11-Deoxycorticosterone 241
2-Deoxy-D-glucose 21
Dexamethasone 303
DHA-sulfate, pregnancy 170
Diabetes 275
- , automatic treatment of acute conditions 339
- coma 339
- , experimental 173
•-, genetic427
- , gonadotropins 121
- insipidus 289
- , rats430
- , streptozotocin 475
- , proinsulinlike material 439
Diaphragm 370
Diarrheic calves 434
Diazoxide, effect on islets 166
Diet 351
- and exercise, catecholamine excretion 532
Dietary carbohydrates, diurnal rhythms
99
Diethylstilbestrol 522
Dihydroergotamine 303
Dihydrotestosterone 89
Diphenylhydantoin 475
Diphosphonate 62
Diuretic effect, glibenclamide 289
Diuretics 115
Diurnal rhythm 99
- variations, corticosterone and cations
329
D,L-ethionine 44
Dog 400
Dogs, hypothyroidism 137
Dopamine neuroleptics 187
Dwarfism 343
- , familial 80
E
Exercise, thyroid hormones 403
Eminence median 94
Entero-insular axis 95
Enzymeactivities, muscle and liver 222
Epinephrine 158
Erythrocytes 158
Estradiol 213,217
Estrogen 208
Estrone 213
- tri tyrosine methyl ester, 1-labelled
334
Estrous cycle 217
Ethanol, effect on glucogon secretion
245
17a-Ethynylestradiol in man 430
Ethynylestradiol, metabolites 333
Exercise, growth hormone secretion 184
- , muscular 488
125
F
Fasting 229,499
Fat cells, free 12
- size 351
Fatty Acid 457
Fenfluramine 85, 86
Fettstoffwechselhormon 36
Fetus 376
F F A 137, 386, 400, 483, 488
Follicle stimulating hormone 121, 171,
335,336,514
Food deprivation 264
XIII
Free Antigen 57
Free Fatty Acids 137, 386, 400, 483,
488
Fructose 370, 457
- , sulfonylureas 284
FSH 121,514
- and L H - urinary 336
- , diabetics 335
- , infertility 171
G
Gastric secretion 517
- ulcer517
, restraint-induced 332
Gastrin 1, 260
Gastrointestinal hormones, effect of 2deoxyglucose 21
Gestation 376
Ghosts, fat cells 238
Glibenclamide 181, 289, 464
- , subcellular distribution 181
Glibornuride 386
Glucagon 1, 49, 70, 392, 443, 478
- , camel 425
- , effect on adenylate cyclase in islets
166
- , pancreatic 156, 396
- , paradoxical release 426
- secretion, neutral red 111
pig 245
Gluconeogenesis 284, 381
Glucose 25, 234, 256, 319, 370, 376,
386, 392, 457, 470
- (3H)12
blood 115, 184
- controlled insulin infusion 339
- , diurnal rhythm 99
- homeostasis 392
- infusion test 400
- uptake, muscle 85, 86
Glucosyltransferase, kidney 427
Glucuronyltransferase 522
Gluconeogenesis 217
Glycemia, Addison's disease 17
- , neutral red 111
- Glycerol 483
~ release 357
Glycogen 370, 457
- content, muscle and liver 222
Glycogenolysis 525
Glycogen synthesis 217
- synthetase 40, 457
Glycolysis 284
Glycolytic metabolites, pancreas 44
Goitre, nontoxic endemic and sporadic501
Gonadotropins, diabetics 121, 335
Group experiment, radioimmunoassay
for insulin 293
Growth hormone 1, 129, 275, 386,506
- , human 184, 303, 347
- , obese mice 125
- , propanolol-insulin Stimulation test
343
- , rat, radioimmunoassay 300
--, responses to physical exercise 428
- therapy 494
Growth rate 494
Guanidine Chloride, insulin secretion
115
Guanidinobutyramide 115
Guanidinobutyric acid 115
Guanidino-propionic acid 115
Guinea pig 267
Gut gli 396
H
HB 419181
HCG, 0-subunit 527
HCS 80
Hemicastration 417
Heparin 151
Hepatic disease, TSH-response 132
HGH, antibodies 484
- , immunological behaviour 80
H-glucose 12
Hindlimb, isolated, rat 167
Histamine assay, T R F treatment 92
Histomorphometry 62
H-lipid counting 12
- extraction 12
Human chorionic gonadotropin (HCG)
527
- placental lactogen 347
Hydrocortisone treatment, Addison's
disease 17
0-Hydroxy butyrate 256
25-Hydroxycalciferol 534
Hyperbaric oxygen 40
Hypercholesterolemia 88
Hyperglycemic mice 40
Hyperinsulinemia 222
Hyperparathyroidism 314
Hyperthyroidism 93, 142, 190, 202, 400
- , cholesterol a cyltransferase 142
- , dogs137
- , treatment 92
Hypocalcemia, parathyroid response 91
- , insulin and glucagon 443
Hypophysectomie 5 3
- , protein kinase 146
Hypothalamic lesion 222
- obesity 370, 454
Hypothyroidism, primary 9
3
3
I
Immunoassay, prolactin 499
Immunological specificity, HCG 527
Immunoreactive insulin (I.R.I.),
Addison's disease 17
Impotence 335
Infertility, male 171
Insulin 217, 219, 234, 247, 256, 272,
280, 357, 376, 386, 392, 443, 457
- administration 222
- antagonist 275
- antibodies 175, 447
- , bioassay 12
- , chronic pancreatitis 95
- , degradation by fat cell ghosts 238
- , diurnal rhythm 99
- , growth hormone 343
- , immunoreactive 400
- infusion, glucose-controlled 339
- , lactating rat 297
- , lipolysis 25
- , oscillations in response 423
- , plasma 115, 165, 184
- , receptors 242
- , recovery in radioimmunoassay 293
- release 526
- requirement 447
Insulin resistance 447
, obese mice 40,125
- , responsiveness 454
- RIA, group experiment 293
- secretion 177,470
, Addison's disease 17
in vitro, local anesthetics 106
, neutral red 111
, pancreozymin 21
- , sensitivity in normal rats 430
- tolerance test 84
- treated diabetes 184
Insulitis 173
Intestinal hormone 272
Intestine 407
Intravenous glucose, chronic pancreatitis
95
- glucose test, rapid 17
- tolbutamide test, Addison's disease 17
Iodide 306
- , hormonal 434
- trapping in vitro 309
IR-HGH 80
IRI, serum 137
Irreversible adsorbent 57
Islets, adenylate cyclase 166
- of Langerhans 49
Isolated fat cells 25
- islets 470
- pancreas 260
- perfused pancreas 256
K
Ketoacidosis 339
Ketone bodies 53
Kidney 407
L
Lactation 297
Lecithin 142
LH 514
- , diabetics 335
infertility 171
L H R F antibodies 94
LH-RH, see also L R H
- , diabetic men 335
- , injection in lambs 240
- Stimulation 336
Lipase, hormone sensitive 247
Lipid counting (3H) 12
- extraction (3H) 12
- metabolism 347,483
Lipodystrophy 447
Lipogenesis 351
Lipolysis 226, 229, 247, 303, 351, 357
- , calcitonin 30
- , isolated fat cells 25
Lipolytic hormone 36
Lipolysis, influence parathyroid hormone and calcitonin 243
Lipoprotein lipase activity, heparin 151
Liver 407,491
- cirrhosis 142
- , enzyme activities 222
- , fructose metabolism 284
- glycogen 381
- membranes 443
- , metabolism 264
- , protein synthesis 280
- triglycerides 53
Local anesthetics 106
L R H , see also LH-RH 510
XIV
L R H , continuous administration 79
- , human males 79
Luteinizing hormone (LH) 121, 410
- releasing hormone (LH-RH) 410
Pituitary, diabetic rats 121
- , "Fettstoffwechselhormon" 36
- function, malnutrition 82
- responses to L R H 79
- respone to LH-RH 240
M
Placenta 280
Magnesium 70, 329
Plasma lipids, diurnal rhythm 99
- deficient rat 91
Polydiethylstilbestrol phosphate 522
Malnutrition 82
Posture, effect on Cortisol 87
Mannoheptulose 21
Potassium 470
Microangiopathy 447
Pregnancy 297
Monocomponent insulin (MC) 175, 447 - , DHA 170
Monospecies pork insulin (MS) 447
Pregnant mice 491, 280
Monthly rhythms, hormones 506
Procaine-HCL 357,457
Muscle 407
Progesterone 217, 297, 430
~, enzyme activities 222
Pro-hormones in tissues and in circula- , glucose uptake 85, 86
tion 1
Proinsulin 1
N
- antibody 439
Nafoxidine522
- -like material 439
Nasal spray 510
Prolactin 187,499,529
Neuroleptics 187
- and testosterone 529
Neurophysin I 83
- , human 347
Neutral red, insulin secretion 111
- inhibiting factor (PIF) 187
Newborn, human 392
- , thyroid diseases 190
Nicotinamide 280
Propranolol 53
Norepinephrine 226
Pro-parathormone 1
Nutrition 226
Propranolol, growth hormone 343
Prostaglandin Ei208
O
Prostaglandins 229
Obese hyperglycemic mice, glucagon
Prostate gland 529
release 426
Prostatic adenyl cyclase 89
- , growth hormone levels 125
Protein-binding assay, 25-Hydroxy- mice 40
calciferoi 534
Obesity, hypothalamic 370
Protein caloriec malnutrition (PCM)
OGTT 137
528
Oligozoospermia, LRH 79
- kinase 49, 208, 247
Oral amino acids load 272
, hypophysectomy 146
- glucose 272
- synthesis 196, 267, 488
, chronic pancreatitis 95
, placenta and liver 280
, tolerance test, Addison's disease Psychoactive drugs 187
17
Psychotropic drugs 530
Osmotic water flow, growth hormone PTH-fragment 1
129
Puberty, girls 325
Ouabain 470
Pyridine nucleotides, pancreas 44
Pyruvate 370, 457
Oxygen consumption, liver 284
- dehydrogenase 457
P
Paget's disease of bone 62
Palmitate 256
Pancreas, metabolism 44
Pancreatectomized rat, partially 275
Pancreatectomy 53
Pancreatic a cell 267
Pancreatitis, chronic 95
Pancreozymin 21
- , chronic pancreatitis 95
Parathormone 1
Parathyroid hormone 243, 314
- , degradation 407
- , radioimmunoassay 407
respone to hypocalcemia 91
Pentobarbital 483
Pentose shunt 457
Perchlorate 306
Perfusion, rat lundlimb 167
Perifusion 470
Phentolamine, effect on islets 166
Phosphatase 247
Phosphate, inorganic 533
Phosphodiesterase 208
Phosphorylase 40
Pig 517
R
Radioimmunoassay, data processing
293
- , quality control 293
- , thyrotropin 9
thyroxine 169
Rana hexadactyla 417
Rat, conscious 115
- , organs, gübenclamide distribution 181
Receptors 443
Red blood cells, potassium 526
Renal Cortisol reabsorption 74
- disease, TSH response 132
- tumors522
Rhythm, diurnal 99
Ribosomes 488
S
S-adenosyl-methionine 333
Salmon calcitonin 161
Secretin 21, 272
- , chronic pancreatitis 95
Sex organs, diabetic rats 121
Sheep 0-lipotropin 303
Skeletal muscle 86
Sodium excretion, urinary 90
- transport, effect of growth hormone
on 129
Somatomedin 125, 319, 494
Steroids 417
- , urinary 325
Stüling cells 417
Streptozotocin 280, 475, 488
Stress 275
Suckling 264
- -food deprivation 381
Sugar, Blood 137,400
Sulfation factor 125
Sulfonylureas 284
Synthetic medium for perfusion 167
T
T 190
TBG, competitive ligand-binding assay
435
- , estimation of 435
Testis compensatory hypertrophy 417
Testosterone 514, 529
- , and prolactin 529
- /cortisol 213
- , endogenous.88
Theophylline 303
Threshold, renal, cortisol 74
Thyrocalcitonin administration 533
Thyroglobulin synthesis 196
Thyroid 306
- activity 93
- , dog 309
Thyroidectomy 84
Thyroid gland, amino acid uptake 196
- hormone levels 528
s in nontoxic goitre 501
Thyroidism, asymptomatic 190
Thyroid secretion rate 403
- , sheep 309
- stimulating hormone (TSH) 410
Thyrotoxicosis 202
Thyrotropin 9, 506
- Releasing hormone 9, 499
, rat 432
(TSH-RH)410
Thyroxine 137, 202, 208
- ( T ) , old age 239
- , radioimmunoassay 169
Tissue culture, calcitonin secretion 70
Toad skin, growth hormone 129
Tolbutamide 386,464, 478
TRF92
TRH 190
- , hepatic and renal diseases 132
- in nontoxic goitre 501
Triamterene 429
Triglycerides 403
Triiodothyronine ( T ) , old age 239
Tryptic peptides and secretin 396
TSH 190
hepatic and renal diseases 132
- , serum, in nontoxic goitre 501
Tubulär maximum, cortisol 74
U,V
Urinary excretion, sodium and calcium 90
Uncinate Dog 478
Vasopressin 1
W,Z
Weanling rat 454
Zinc 329
3
4
3
293
Horm. Metab. Res. 6 ( 1 9 7 4 ) 2 9 3 - 2 9 6
© Georg Thieme Verlag Stuttgart
Group Experiments on the Radioimmunological Insulin Determination*
1
1
1
1
1
I. Marschner , P. Bottermann , F. Erhardt , R. Linke2, G. Löffler , V . Maier3, P. Schwandt ,
W. Vogt , and P.C. Scriba
1
1
1
Sonderforschungsbereich 51 Muenchen, and Sonderforschungsbereich 87 Ulm ( München, 2j tzing, ^uim),
Germany
u
Summary
By means of two group experiments, performed in seven
laboratories and using six different modifications in the insulin radioimmunoassay technique, it is shown that the use
of Standards diluted in human serum of low basal insulin
concentration, instead of buffer, improves appreciably the
interlaboratory precision. The causes lies perhaps in some
serum factor, which could disturb some modifications of
the radioimmunoassay more than others. There was no improvement of precision when the same insulin Standard was
used in all laboratories. Standard curves made up in different human fasting sera have a scatter only within the
ränge of pipetting and counting error.
Key-Words: Group E x p e r i m e n t - I n s u l i n R I A - D a t a
cessing - Q u a l i t y C o n t r o l - I n s u l i n
Recovery
Pro-
Introduction
Elimination of inter-laboratory differences of the
results of clinical chemical analyses has recently
drawn major attention. Quality control of routine
methods largely is performed by means of participation in group experiments. The aim of this work
was to design and to test a modell for collaborative, comparative studies on the radioimmunological
insulin determination with the goal of improving the
inter-laboratory precision.
in his own buffer system, and using his usual concentrations. The unknowns were then evaluated with the laboratory's own Standard curve, as well as with the Standard
curve containing the common insulin Standard.
Second g r o u p e x p e r i m e n t ( 1 9 7 3 ) : The samples assayed in
the second group experiment were as follows:
Nine samples from a new serum pooL
Triplicates of seven serum samples drawn from a further patient during an OGTT. Times of venipuneture: 0, 5, 1 0 , 15,
20, 3 0 and 6 0 minutes after administration of glucose.
Triplicates of 8 samples to form the recovery curve. These
were made in human serum with a low basal concentration
of insulin. For this we used the serum of a fasting patient
which contained approximately 6 M U insulin/ml. 2 0 0 /utU
insulin were then dissolved in each millilitre of an aliquot
of this serum, so providing a stock serum Standard of 206
M U insulin/ml. From this, further serial 1 + 1 dilutions were
made, using the rest of the low insulin serum as diluent.
Thus the following Standards were obtained 206, 106, 5 6 ,
3 1 , 18.5, 12.3, 9.2, and 6 /uU/ml. In all cases the identity
of the samples was withheld from the partieipants.
Results
First group
experiment
The individual group modifications of the insulin
RIA technique are shown in Table 1. Because all
unknowns were measured in one assay-run, the within assay coefficient of Variation could be obtained
from the 24 samples of the serum pool. This ranged
between 3.4% and 14%. The scatter of the means of
the 24 values from each laboratory was between 21
Material and Methods
juU/ml
and 46 /iU insulin/ml and had a coefficient
All experiments were performed on the same samples by
of Variation .of 21%. The results from the OGTT-sera
seven individual groups of workers in different laboratories.
had a similar scatter. The means of the measured inFirst g r o u p e x p e r i m e n t ( 1 9 7 2 ) : For the first group experiment we dispatched the following two lots of serum sampsulin concentrations lay between 13 juU (fasting) and
les to all seven partieipants.
82 /iU/ml (60 minutes after glucose), and the coeffiTwentyfour samples from a serum pool, mixed from the serum cient of Variation of 18% calculated in the lower conof 5 patients, venipuneture one hour after breakfast.
centration ränge increased to 33% in the higher concentration ränge. The result from the first group exSi> serum samples with different concentrations of insulin,
taken from one patient during an oral glucose tolerance test periment was, that there was no improvement of
(OGTT). The venipunetures were performed at 0 , 5, 10, 2 0 , inter-laboratory precision, when the same insulin Stan30 and 6 0 minutes after the administration of 100 gm gludards were used by all groups.
cose. Each sample was assayed in triplicate. In addition,
each box with the frozen samples contained a vial of
Second group
experiment
2 nU insulin Standard preparation (kindly supplied by
Because of the different methods for evaluating RIA
Fa. Buchler, Braunschweig - RCC, Amersham). With this
data (logit- or parabola-transformation, graphic-manual
insilin Standard each partieipant prepared a Standard curve
procedures), which intrinsically yield different hor*Sipported by the Deutsche Forschungsgemeinschaft
mone concentration values, particularly at either end
(SFB 5 1 , München, and SFB 87, Ulm).
of the Standard curve, all results were handled as raw
Received: 15 Feb. 1974
Accepted: 12 Mar. 1974
counts in this second group experiment in contrast
294
I. Marschner et al.
Fig. l a Results of the insulin-determinations of the serum pool (n=9) as calculated from each laboratory's own Standard
curve in buffer (second group experiment). The results from each laboratory are represented in a Gaussian-curve. As usual
the peak of each curve represents the mean value, the width of each curve between upper and middle third is two Standard
deviations. The peaks (means) have a scatter with a coefficient of Variation of 28%. The results of the first group experiment (cf. text) were similar.
Fig. l b The same data, related to the recovery curve, i.e. Standards dissolved in low insulin serum. Coefficient of Variation
of the means 8%.
to the first one. In this way we were able to evaluate
all hormone concentrations using one method, and so
record only the differences of the assay variations.
This was done with a Computer program, using spline
approximation to fit the Standard curve. A Siemens
404/3 data processing machine was employed. The
curves were plotted by an on-line controlled Hagenplotter (Marschner,
E r h a r d t and Scriba 1973). The
count rates of the unknowns from the various
groups were related firstly to their corresponding
Standard curve and then to their recovery curve.
Calculation
o f the unknowns,
using
the S t a n d a r d
curve i n buffer
The means of the 9 serum pool samples from all
seven groups had a scatter with a coefficient of Variation of 28%. This, and also the individual within
assay precision were not appreciably different from
the results of the first experiment (Fig. la). Similar
results were also obtained by comparing the hormone values from the OGTT in both group experiments (Fig. 2a).
C a l c u l a t i o n of the unknowns,
using the recovery
curve
By calculating the insulin concentrations of the samples from the serum pool (Fig. lb) and those from
the OGTT (Fig. 2b) using the recovery curve (see
above) we obtained a great improvement of interlaboratory precision. Inspite of the fact, that the
individual within-assay precision remained nearly uninfluenced, the scatter of the means of the pooled
serum was reduced to a coefficient of Variation of
only 8% from 28%.
It was noticed, that the recovery curves in serum
generally had a gradient steeper than the Standard
curves in buffer (Fig. 3).
Table 1 Modifications of the radioimmunological determinations used by the partieipants.
Incubation
Temp.
Ml
PreIncubation
h
h
°C
A
B
100
100
6
6
24
18
C
100
-
D
100
E
Serum
Volume
Pipetting
Steps
B/F-Separation
4
4
3
3
Filtration on Cellulose Acetate Filters,
Using Preprecipitated Antibody
17-24
4-8
2
Solid-Phase Technique Fa. Boehring;r
-
72
4
4
Ion Exchange Resin (Amberlite)
100
-
24
22
6
Sepharose-bound Antibody Pharmacia,
Uppsala
F
50
3
22
3
Sephadex G-75-Column Chromatogaphy
G
100
-
37
3
Dextran Coated Charcoal Adsorption
8
120
295
Group Experiments on the Radioimmunological Insulin Determination
MICHROUNITS INSULIN/ML
MICROUNITS INSULIN/ML
120-h-
120-
110»- •
110--
100 J-
100
•
Füg. 2a io
00 MIN
Fig. 2b io
MINUTES
Fig.. 2a Results of the insulin-determinations in the sera from the OGTT, related to each laboratory's own Standard curve
in buffer. Ordinate: insulin concentration in pU/ml. Abscissa: time of venipuneture after glucose administration. The middle
line* connects the means of all Single values of all laboratories, the shaded area covers two Standard deviations. Mean coefficiemt of Variation 14%.
Fig.. 2b The same data, related to the recovery curves: mean coefficient of Variation 6-5%. In the higher concentration
ränge of the Standard curve no improvement was obtained (decreasing steepness).
We examined the usefulness of various human fasting
sera for insulin recovery curves and prepared 7 recovery curves as described above. Triplicates of 4
comtrol samples calculated by the Computer on each
of the 7 recovery curves, showed a coefficient of
Variation not greater than 7%, which lays within the
precision of the assay.
8[%|
RECOVE RY C: U R V E
\
Discussion
S T A N D A R D CU RVE
Group experiments are a well aeeepted procedure to
examine the validity of laboratory results. For ordinary laboratory methods one or two determinations
of a substance are sufficient for such a project whereas because of the nonlinear RIA Standard curves 6 to
8 determinations in triplicates of samples having concentrations which are spread over the sensitive part
of the Standard curve are essential.
\^\^
\\
It is desirable, that the concentrations of the samples
are within a physiological context, like in our case
from an OGTT.
To make sure, that the results represent all individual laboratory errors, each partieipant should handle
his samples as in a routine assay. A detailed Information about the individual assay is not necessary. The
only important fact is the agreement of results with
those of other laboratories.
Since one is commonly interested in comparing assay Systems and not the different evaluation procedures, it is advantageous to ask for count rates instead of hormone concentrations. From the obtained count rates the hormone concentrations will be
0
3.13
6.25 12.5 25.0 50.0 100.0 200.0
MICROUNITS INSULIN / ML
Fig. 3 A typical Standard curve in buffer and recovery
curve. The latter has a steeper gradient. Ordinate: B / B
(B = antibody bound activity, B = maximal antibodybound activity in absence of cold hormone). Abscissa:
logarithmic dose (/uU insulin/ml). The horizontal line at
87% is the limit of sensitivity, calculated as 3 Standard
deviations of B -values (n = 9).
0
0
0
296
I. Marschner et al.
It is apparent, that the precision will decrease a little,
if each laboratory uses a different serum for its own
Standards. Despite this, our results suggest, that this
decrease would not be great. It is, furthermore recommended that Standards be made by serial dilutions
of 1 + 1, starting from one stock Solution containing
about 200 to 300 juU/ml. Thereby, it is necessary to
The two group experiments, regarded as pilot studies,
use only one pipette and one Single volume, resulting
show, that both the type of the RIA System (antiin a precision superior tc all other ways of pipetting
body, bound-free-separation, incubation-time and
Standard dilutions.
-temperature, sample volume) are of secondary importance in improving inter-laboratory precision. It
References
is, therefore, less important to standardize either of
these. Differences in the purity of the tracer, the in- A s h f o r d , W.R., J . C a m p b e l l , J . K . D a v i d s o n A M . F i s h e r , R.E.
H a i s t , A M . Lacey, B . L i n , J M . M a r t i n N i n a H . M o r l e y ,
cubation and the antigen-antibody-reaction seem to
K . Sudha R a s t o g i , a n d W.O. S t o r v i c k : A consideration of
play a greater role in serum samples than in pure
methods of insulin assay. Diabetes 18 (1969) 823-833
buffer Systems. In view of this, it was considered
E r h a r d t , F . , I . M a r s c h n e r , Renate C. P i c k a r d t , P . C . S c r i b a :
better to dissolve the insulin Standard not in buffer,
Verbesserung und Qualitätskontrolle der radioimmunologischen Thyreotropin-Bestimmung. Z. klin. Chem. 11
but in serum with a low basal insulin concentration.
(1973) 381-387
Comparable effects have been shown by E r h a r d t ,
M a r s c h n e r , /., F . E r h a r d t , P . C . S c r i b a : The calculation of
Marschner,
Pickardt,
and Scriba (1973) in the measthe radioimmunoassay Standard curve by "spline funcurement of hTSH.
tion". Symposion on radioimmunoassay and related piocalculated in a uniform fashion by an appropriate
Computer program. From the count rates of the
Standard curves one gets also Information about the
steepness of the slope of the Standard curves and
the limits of sensitivity (threefold Standard deviation of the zero-standard).
A coefficient of Variation greater than 20% is considered too high for satisfactory hormone measurement, particularly when needed frequently, for clinical and research purposes and when the RIA itself
is relatively simple (Ashford
et al. 1969). This can
be improved considerably, however, by using Standards in serum. In this way the inter-laboratory precision approximates to the ränge of such assays as
cholesterol or urea-nitrogen (Stamm
and Büttner
1969).
cedures in clinical medicine and research. Internationa
Atomic Energy Agency, Sept. 1973. Istanbul, Turkey
Stamm,
£>., H . Büttner: Ringversuch zur Qualitätskontrolle
1968. Z. klin. Chem. 7 (1969) 393403
Requests for reprints should be addressed to:
Dr. I. Marschner,
Laboratorien für Klinische Chemie und Endokrinologie
II. Medizinische Klinik der Universität
D-8000 München 2, Ziemssenstr. 1 (Germany).