1. Art and Diseño

CURRICULUM VITAE
(2015 January)
Name:
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Carlos
Noceda Alonso
EU Marie Curie Chair
Instituto de Ciências Agrárias e Ambientais Mediterrânicas (ICAAM)
Laboratory of Molecular Biology-University of Évora
Apartado 94, 7002-554 Pólo de Mitra, Évora, Portugal
Navarro-Campo Iglesia-Casas Carr., 17
33490 Avilés (Asturias).
Spain
Spain
AD706202
male
[email protected]
(+34) 606953023, (+351) 963320884
DRIVING LICENCE Class B (for cars) from 13.01.1984
Disposure for travelling
RESEARCH INTERESTS
I am a plant molecular biologist with experience in tissue culture. My education (PhD in
Biology and MSc in Plant Biotechnology) and international research experience have provided me
a broad understanding of plant cellular, molecular and evolutionary biology. This covers genetic,
epigenetic, transcriptional and biochemical studies on developmental biology and (epi)genetic
variation, frequently with the applied aim of improving plant cultures. My recent researches deal
with molecular studies of developmental stages and search of functional markers of stress
resistance, frequently from an evolutionary approach. My main goal from here onwards is to use all
that knowledge to go more deeply into eco-evo-devo mechanisms, and, if possible, to use the
arising information for applied purposes. I am sure that my background not only will be adaptable
to the objectives of your institution, but also will provide additional approaches.
Since the period of my PhD very often in my career I had to spend time setting up different
methodologies from the beginning of the researches, always with success to be used for
experimentation or analysis. For example, in my initial researches in plant biology at the Plant
Biotechnology Institute of Santa Clara (Cuba) –developed as a grant holder from the Spanish
International Cooperation Agency– I had to deal with unknown factors affecting propagation and
quality of plant field and in vitro cultures. During several years, always under fundamental and
practical approaches, I studied distinct molecular profiles –metabolites, released proteins,
isozymes- in different plant developmental processes to find biochemical indicators and
characterizations of morphogenetic capacities and physiological stages, and to establish
associations with variant genomic (AFLPs, functional markers) and epigenomic (MSAPs) molecular
markers. In this way, at the University of Oviedo (Spain) we found an inverse relationship between
pine genetic potential for somatic embryogenesis and both global genomic methylation and
polyamine contents. Nevertheless, these and other molecular associations were found to do not
exist regarding the typical loss of embryogenic ability with ageing of the cultures of several pine
genotypes, in a study in which I took actively part at Canadian Forest Service-Québec under the
supervision of Dr. Krystina Klimaszewska. Subsequently, I went into detail analysing more
treatments with respect to genome methylation changes, especially at INRA-Orléans (France), but
results are still being processed. Those studies on genome methylation regarded percentage of
methyalted cytosines and methylation patterns.
I also participated in the development and validation of a plantain micropropagation method
in a European Union research project for International Cooperation, which main researcher was
Prof. Pierre Debergh, from the University of Gent. In addition to biotechnological work, my role
there consisted mainly in the molecular assessment of field regenerants, performing karyotypic,
genomic and epigenomic analyses to follow culture-induced morphological somaclonal variation.
We found an AFPL marker associated to dwarfism. In that time I also cooperated in the molecular
characterization of artificial banana/plantain hybrid lines.
From recently I worked at the University of Évora (Portugal), under the supervision of Profs.
Birgit Arnholdt-Schmitt and Augusto Peixe. Here I continued working on my interests around
epigenetics, thus starting a research on developmental and evolutionary involvement of the
changing structures of the genome. From this perspective I studied, for example, variations in
number of DNA repeats under distinct culture conditions and in different plant tissues and
individuals. Keeping an eye on evolutionary approaches, I also have searched for functional
markers of plant stress resistance, so important in fitness and consequently highly linked to the
evolution, especially of reproductive systems. My current work consists in the study of the
differential regulation of gene expression (by RT-qPCR) during morphogenetic processes in plants
with different potential for adaption, with an emphasis in the alternative oxidase function and
genes, which are involved in physiological adaptations to biotic and abiotic stresses.
Currently I´m a Prometeo (Senescyt, Ecuador) researcher on molecular basis of somatic
embryogenesis of banana and coffee tree.
During all those researches I have acquired expertise in the use of bioinformatic platforms
and tools to analyse nucleic acid and protein sequences, having also a basis in biostatistics. In
spite my research work was mainly performed under a plant-biotechnology basis, I have also
worked in physiology and pathology of fishes and mammals. Furthermore, my formation in
sciences is not reduced to Biology, since I studied several university courses on Physics and,
furthermore, I taught sciences (Mathematics, Physics, Chemistry, Biology) at high school and
university levels.
The above mentioned stances abroad, so as international meetings and writing of
scientific papers have provided me a good level of spoken and written English, so as a basic level
of Portuguese and French, being Spanish my mother language. My communication skills have
increased with activities such as national and overseas pre-graduate and postdoctoral researcher
training and supervision, and management of projects. It is also worth to say here that I obtained
the Pedagogical Attitude Certificate awarded by the Spanish Institute of Educational Sciences.
I am highly interested in put in practise my knowledge and skills and co-operate in the
emergent development of science in Ecuador, hereby contributing to the social welfare which is
expected from the current politics. Summarizing, initiatives for working in universities or research
centres of Ecuador, like contracts or the Prometeo project, perfectly suits my professional and
personal interests, and I would fill very comfortable in the work.
WORK EXPERIENCE (EMPLOYMENT)
Research/Academy
Participation in research projects
- Current: “Caracterización de alteraciones moleculares que originan variación somaclonal”
Prometeo consultant (2014 Feb-2015 Feb).
Centro de Investigaciones Biotecnológicas del Ecuador (CIBE)- Escuela Superior Politécnica
del Litoral (ESPOL). Guayaquil. Ecuador.
Main Reseracher: Esther Lilia Peralta
Standardization of methods for estimation of (epi)genetic variability on the basis of observed
molecular changes. Applications in optimization of micropropagation protocols.
- “Adventitious rooting in olive cuttings: a physiological response under oxidative stress?”
Postdoctoral researcher (2012 May-2013 Dec)
Instituto de Ciências Agrárias e Ambientais Mediterrânicas (ICAAM). Laboratory of
Molecular Biology and Laboratory of Phytotechnics-University of Évora. 7002-554 Évora,
Portugal
Main researcher: Augsuto Peixe
Study of the regulation of gene expression during plant morphogenetic processes -mainly
rooting-, with an emphasis in their control by the alternative oxidase function and genes,
which are involved in physiological adaptations to biotic and abiotic stresses. Measurements
of respiratory enzyme capacities, use of bioinformatic tools and platforms; nucleic acid
extractions, (RT)-PCR, electrophoresis; DNA fragment purifying, cloning and sequencing;
data analyses and evaluation.
- “Characterization of alternative oxidase (AOX) polymorphisms in Hypericum perforatum L.
– A novel approach to advance breeding on yield stability in a medicinal plant”
Researcher (2011 May-2012 Apr)
EU Marie Curie Chair. Instituto de Ciências Agrárias e Ambientais Mediterrânicas
(ICAAM). Laboratory of Molecular Biology-University of Évora. 7002-554 Évora, Portugal
Main researcher: Birgit Arnholdt-Schmitt ([email protected])
Study of developmental and evolutionary involvement of the changing structures of the
genome: e.g., variations in number of DNA repeats. Setting up of molecular biology
techniques (Southern blotting). PCRs. Study of the contribution of 3'-UTR of genes to gene
and mRNA function, and possible implications in the search for functional markers. Keeping
an eye on evolutionary approaches, search for functional markers of plant stress resistance.
Standardization of a method for in vitro culture of H. perforatum in order to serve as a model
for biological assays and molecular analyses.
- “Evolution de la méthylation de l’ADN de lignées embryogènes de pin maritime au cours
du processus de multiplication conduisant à la perte progressive de leur capacité de
maturation”
Postdoctoral CDD contract (2009 Mar - Dec ).
Unité Amélioration, Génétique et Physiologie Forestières. *Institut National de la
Recherche Agronomique (INRA), 2163 Avenue de la Pomme de pin, CS 4001 Ardon F- 45
075 Orléans Cedex 2 France
Main researcher: Marie Anne Lelú-Walter
Main goal: Epigenetic characterization of Pinus pinaster cultures with distinct embryogenic
potential. This included analyses of global DNA methylation levels and mRNA accumulation.
The duties included: degenerated primer design for candidate genes, molecular database
search, use of bioinformatic tools; reception, storage and processing of samples (DNA
extraction, DNA hydrolysis); measurement of global DNA methylation -by High Performane
Liquid Chormatography (HPLC)-, PCR, Gel electrophoresis; DNA fragment purifying, cloning
and sequencing; data analyses and evaluation, and report writing in English.
- The role of epigenetics in long term somatic embryogenesis of conifers
Visiting Scientist Contract (2006 Feb -2006 Oct ).
*Natural Resources Canada, Canadian Forest Service (NRC-CFS), Laurentian Forestry
Center, rue du P.E.P.S., 1055, Sainte-Foy G1V 4C7, 10380 Québec, Canada.
Main researcher: Krystyna Klimaszewska ([email protected])
Main goal: Epigenetic and biochemical characterization of conifer cultures with distinct
embryogenic potential (regarding genotype, age of culture, storing, type of somatic
embryogenesis, treatment with demethylating agents). This included analyses of both global
DNA methylation and polyamine levels. The duties included: use of bioinformatic tools;
collecting of explants, tissue culture for somatic embryogenesis, storage and processing of
samples (RNA and DNA extraction, DNA hydrolysis); measurement of global DNA
methylation - by High Performance Performane Capillary Electrophoresis (HPCE)-, PCR, Gel
electrophoresis; analysis of Methylation Sensitive Amplyfication Polymorphisms –MSAPs-,
DNA and RNA quantifying, quantitative RT-PCR, analyses of free and bound polyamines by
High Performance Liquid Chormatography (HPLC), data analyses and evaluation; review of
literature; article writing and conference communication, both in English.
- International INCO Project of the European Union UE-01-ICA4-CT-10063: Scaling-up
plantain production and quality by Temporary Immersion Bioreactor (TIB)-technology:
validation of this plant propagation procedure.
Postdoctoral Contract of Formation of Research Staff (2002 Nov-2005 Sep).
*Laboratory of Plant Physiology, Department of Biology of Organisms and Systems,
University of Oviedo, Spain.
Short stays in:
*Corporación Bananera Nacional (CORBANA). Guápiles. Costa Rica, 2004 Ago
02-09. Sampling and Project meeting.
*Centro Agronómico Tropical de Investigación y Enseñanza (CATIE). Turrialba.
Costa Rica, 2004 Ago 10-15. DNA extraction and preparation of samples for
transport.
Participation in meetings of the Project
* Ciego de Ávila, Cuba (2003)
* Oviedo, Spain (2004)
* Guápiles, Costa Rica (2004)
Director: Prof. María Jesús Cañal ([email protected]).
Goals: Detection of somaclonal variants and molecular characterization of field and
micropropagated phenotypes of plantain, identification of genetic markers and improving of
micropropagation methods stating the quality of the regenerants, specially regarding
organogenic multiplication. The duties included:
-Checking of genetic and epigenetic variability in macro and micropropagated Musa studding
a) nuclear DNA contents, including ploidy levels -using flow cytometry-, b) genetic alterations
using Ag staining and fluorescence-based fluorimetry, and c) quantitative changes of global
genomic DNA methylation using HPCE after hydrolysis.
-Quantifying of free and bound polyamine contents –by HPLC- in order to discuss a possible
relationship with molecular variations; study of different tissues and different stages of
micropropagation process using different culture conditions in both semisolid medium and
temporary immersion bioreactor systems (BITs).
-Validation of BIT-regenerated plants by evaluation of plant production rate and quality; study
of different culture conditions (growth regulators, physical factors…) for allowing the
optimization of the methodology, supported by histological observations.
-Literature review; Diffusion of results; training and supervision of researchers and students;
conference organizations and assistance to conferences and project international meetings,
in both English and Spanish.
Minor research collaborations
- Departament of Functional Biology of the University of Oviedo:
* Acid gastric secretion" and "Changes in the sexual behaviour of rat induced by propanolol
adrenergic inhibition. (Ref.: Emilia Menéndez Abraham, Área of Phyisiología Animal). 1991
* Population Genetics of Trifolium repens (Ref.: M.A. Comendador, Area of Genetics).
- IBP-UCLV, Santa Clara (Cuba)
* Research on somatic embryogenesis of Coffea.
Co-operation in the organization of meetings
- Meeting of the Society for In Vitro Biology, Minneapolis (2006)
- Reunión Proyecto Europeo, Oviedo (2004)
- COST 843 Oviedo (2004)
- BIOTEC 2004, Oviedo (2004)
Training and supervision of researchers
- Fernández O (2007) Somaclonal variation of Musa AAB clone ‘CEMSA ¾’: morphoagronomic
descriptors, molecular markers and biochemical indicators. Bachelor Thesis. University of
Oviedo. (Highest mark). Supervisors: Cañal MJ and Noceda C.
- Pérez Suárez M (2006) Genomic DNA methylation and free polyamine contents as indicators of
somatic embyogenic potencial in Pinus nigra Arn. Bachelor Thesis. University of Oviedo.
(Highest mark). Supervisors: Rodríguez R and Noceda C.
- Santamaría E (2006) Plant production of Musa AAB clon CEMSA ¾ by temporary immersion
systems: validation of regenerants. Master Thesis. University of Oviedo. (Highest mark).
Supervisors: Rodriguez R and Noceda C.
- Santamaría E (2004) Plant production of Musa AAB clon CEMSA ¾ by organogenic
micropropagation: validation of regenerants. Bachelor Thesis. University of Oviedo. (Highest
mark). Supervisors: Cañal MJ and Noceda C.
Teaching
- “Introducción a la Fisiología Vegetal” and “Genética”, from the career de Ingeniería Agrícola
y Biológica-, 2014, ESPOL, Guayaquil, Ecuador
- Sciences and Humanities (High School level), including Mathematics, Physics, Chemistry,
Biology and English. Olimpo Academy, Avilés, Spain (2008-2009).
- Plants as biofactories: present or future? Program for Seniors of University of Oviedo
(PUMUO), Spain (2004 and 2005)
- Support in theoretical and practical teaching on Plant Physiology in the Biological Sciences
university career. University of Oviedo (2002-2005)
- Conference in a Ph.D. Program of the Department of Biology of Organisms and Systems.
University of Oviedo, Spain (2005)
- Teaching in the course “Expert in the Collection of Urban Solid Wastes" (300 lecture hours)
(1994). Ayuntamiento de Avilés, Asturias (Spain)
- Mathematics (High School level) (1990). LECI Academy, Gijón, Spain
- Collaboration in the making (design, screenplay, filming and edition) of a didactical video on
Environment (1990). Promoted by the Associated Students of Biology (EBA) youth
association, which belonged to the Students of Asturias (EDA) federation, a member of the
Youth Council of the Principado de Asturias, in collaboration with the Audiovisual Media
Service of the University of Oviedo
- Field teacher of Biology and Geology (High School level) EBA and Monte Naranco Institute.
Arriondas, Asturias, Spain (1989). Into the environmental education Programme of Itineraries
in the Nature as support to the High School teaching.
- Others: Natural Sciences, Nutrition, Drawing, Biohemictry, Biostatistics, Genetics, Chemistry and
Algebra of universitary studies.
Technical/Commercial
- Public Relationships (2002) Urbatel Marketing Directo, SL, Oviedo, Spain.
- Enquirer for statistical researches (1995). Random (opinion, marketing and socio-economic
studies), Madrid
- Local Development Agent (1994-1995), into the Program "Attending the Cultural Change”
(Spanish Federation of Counties and Provinces -FEMP-), financed by the European Union.
Co-ordinator of the team elaborator of the Study on environmental conservation:
"Planning for the extension of selective collection of urban solid wastes in the county of
Avilés”, city Council of Avilés, Asturias, Spain.
- Collaborations with ICONA (Spain) in works for environmental control (1985-1988).
- Insurance seller (2002 Sep-Oct)
PROFESSIONAL SPECIALTY AND INTERESTS
Research lines
Molecular/functional markers, somaclonal variation, genome changes, gene expression, DNA
methylation, extracellular proteins, polyamines, tissue culture, somatic embryogenesis
Research topics
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Somatic embryogenesis
Organogenesis
Plant ageing and reinvigoration
Polyamines
Peroxidases and extracellular proteins related with somatic embryogenesis
Somaclonal variation
Molecular markers
Functional markers
Epigenetics (DNA-methylation)
Gene expression and morphogenetic control
Genome changes and evolution
Species:
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Daucus carota
Oleas europaea
Coffea spp.
Hypericum perforatum
Musa spp.
Pinus spp.
Picea spp.
TECHNICAL AND PROFESSIONAL QUALIFICATIONS
- Membership of Biologist Official College (COB, Spain) from 1990.
- Pedagogical Aptitude Certificate (CAP) (1990) Institute of Educational Sciences (ICE),
University of Oviedo, Spain.
- Title of Projector-Installer of Sun Energy (1992). Centre of Solar Energy Studies (CENSOLAR),
authorised by the Ministry of Education and Science, Spain.
SKILLS
Techniques and equipment (with all related software):
Routine handled:
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Plant Tissue and Cell Suspensions Culture (Somatic Embryogenesis and Organogenic
Micropropagation): Laminar Flow Chambers (1996-1998, 2002-2006) Bioreactors,
Temporary Immersion Systems (TIB®), Automated Temporal Immersion Recipients
(RITA®), cell-based screening techniques, etc. Growth chambers.
Ultracentrifuges: 1998-1999, 2002-2006
Spectrophotometry
Flow cytometry (Beckman Coulter’s Cytomics FC 500) (2003-2005)
Extraction and purification of secondary metabolites, proteins and nucleic acids.
Separation of polyamines (HPLC -Waters®:1998-2001, 2002-2005, nucleosides (HPCE
-Waters®: 2002-2005, proteins (SDS-PAGE) and nucleic acids (electrophoresis in
agarose gels)
Protein identification: Immuno-analytical techniques Dot and Western blot.
Genome and epigenome analyses: restriction endonucleases, PCR, nucleic acid
cloning, sequencing and analysis, molecular markers (AFLPs -ABI Prism® 3100
Genetic Analyzer: 2005, MSAPs -Li-Cor®: 2006), analyses of global DNA methylation
(HPCE -Waters®: 2002-2005; HPLC: 2009)
Gene expression analysis: semiquantitative RT-PCR (2009)
Occasionally handled, or training:
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Genome and epigenome analyses: chromosome walking (2011), Southern blotting
(2012)
Optical and transcription electronic (TEM-1993-1994) microcopy.
Gene expression analysis: RT(q)-PCR (2006, 2011, 2012, 2013)
Oxigraphy
Project Management Skills
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Writing of scientific research proposals
Designing and execution of projects
Supervision of project teams
Analysing of obtained information
Writing of technical and scientific reports
Languages
- Spanish (mother language)
- English: stances in USA (accumulated: 2 months), UK (accumulated: 3 months) and Canada (9
months):
* Course by mailing with The English School (1985).
* Couse and Certificate of the Stanton School of English (1992 Nov 11-1993 Jan 03). Level
4 (Higher intermediate: Oxford and Cambridge Preliminary) of 6. London.
* Course in the Dundee College (24 hours) (1995 Jul 31- Aug 18). Advanced. Dundee,
Scotland
- Basic knowledge of French (stances in France): several courses in France.
- Basic knowledge of Portuguese (stances in Portugal).
- Intensive course on Finish Language and Culture (1993 Aug 02-15). Helsinki
Computing
- Course on mechanic design with AutoCAD (Computer Aided Design) applications (480 hours)
(1991 Jan 10-Jul 19). National Institute for Employment (INEM). Oviedo, Spain. Scholarship
of INEM.
- Course on BASIC programming language (1985 Jan 01-May 31). Centre for Informatics
Assessment and Instruction, Oviedo, Spain. Diploma (1985 May 31)
- Operative systems (MS-DOS, WINDOWS), integrated packages, word processors, data
bases, computer sheets, Internet.
- Software applied to different analytical techniques: HPCE and HPLC (Waters©), DNAsequencing, DNA-fragment analysis, Li-Cor®, RT-PCR.
- Bioinformatics: protein and genome data bases, degenerate and non-dgenerate primer design,
multiple alignement.
- Statistical applications (SPSS and its CHAID module; SigmaStat)
- Statistical and experimental design knowledge and experience
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EDUCATION
- Ph.D. Biology (1991-2001) University of Oviedo, Spain. Thesis on Plant Biology. Program
(1991-1993): "Oceanography and Biology of Aquatic Resources”.
- M.Sc. Plant Biotechnology (1996-1999) Central University “Marta Abreu” of Las Villas, Cuba.
Program in Institute of Plant Biotechnology. Scholarship of the Spanish Agency for International
Cooperation (AECI) (Avenida de los Reyes Católicos, 4, Madrid, Spain).
- Grade of Bachelor in Biological Sciences (1990) University of Oviedo, Spain.
- Bachelor in Biological Sciences (speciality: Fundamental Biology) (1983-1989) University of
Oviedo, Spain.
Workshops/courses/only attended meetings
Scientific/Technical
- Bioveg (2007). Advanced Techniques Applied to Mass Clonal Propagation of Plants. May 711. Centro de Bioplantas, Ciego de Ávila. Cayo Coco (Cuba)
- BioVeg (1997) International Workshop on Plant Biotechnology. Apr 2-5. Centro de Bioplantas,
Ciego de Ávila (Cuba)
- IV Coloquio internacional de Biotecnología de las Plantas (invited, 1996). Jun 23-26. Instituto
de Biotecnología de las Plantas, Santa Clara (Cuba).
- Courses on both Physics (National University for Distance Education –U.N.E.D.-, Spain 19941995) and Biochemistry (University of Oviedo, Spain, 2004-2005) university careers
- Television and Video production (18 hours) (1994 Nov 14-19) Youth Association Rigel, Gijón,
Spain.
- Title of Projector-Installer of Sun Energy (1992). Centre of Solar Energy Studies (CENSOLAR),
authorised by the Ministry of Education and Science, Spain. Financed by a partial grant of
this institution
- Food microbiology (25 hours) (1991). Official College of Veterinary of Asturias, Oviedo, Spain.
- I Practical Course on “In vitro culture of Plant tissues” (68 hours) (1990 Jul 9-20) University
Extension, University of Oviedo.
- "Knowing the human brain” (14 hours) (1990). University Extension, University of Oviedo,
Avilés, Asturias, Spain.
- International course on Environmental Education (15 days) (1989 Jul 16-31) University
Student Association of Extremadura (AUNEX). Hoyos, Cáceres, Spain. Burse for travelling of
University of Oviedo, trough the Biology Students Association (EBA).
Pedagogy
- Course on Formation of Formers of “Expert in Cultural Change applied to the Separation and
selective Collection of Urban Solid Wastes (180 hours) (1994 Aug 29- Sep 23). Program
for the Cultural Change “University and County”. Coordinated by the Spanish Federation of
Counties and Provinces (FEMP) Zaragoza, Spain.
- I Journeys on Communication Media and Youth, with workshops of TV and video (1990 Oct 26). Assessor Council of the Spanish Radio and Television – RTVE- of Asturias and the Youth
Council of the Principado de Asturias. Oviedo, Spain.
- Pedagogical Aptitude Certificate (CAP) (1990 May 02) Institute of Educational Sciences (ICE),
University of Oviedo, Spain.
PUBLICATIONS
INDEXED PUBLICATIONS
Research articles
- Noceda C, Vargas A, Roels S, Cejas I, Santamaría E, Escalona M, Debergh P, Rodríguez R,
Sandoval J, Cañal MJ (2012) Field performance and (epi)genetic profile of plantain (Musa
AAB) clone ‘CEMSA ¾’ plants micropropagated by temporary immersion systems. Sci Hortic
146: 65-75. doi 10.1016/j.scienta.2012.08.007.
- Cejas I, Capote I, Aragón CE, Escalona M, Pina D, González J, Rodríguez R, Noceda C, Cañal
MJ, Sandoval J, Roels S, Debergh P (2011) Optimizing protocol of plantain propagation cv.
CEMSA 3/4 in Temporary Immersion Bioreactors. Agrociencia (Montevideo) 15(1):13-18.
- Noceda C, Salaj T, Pérez M, Viejo M, Cañal MJ, Salaj J, Rodríguez R (2009) DNA demethylation
and decrease on free polyamines is associated with the embryogenic capacity of Pinus nigra
Arn. cell culture. Trees 23: 1285-1293. doi 10.1007/s00468-009-0370-8.
- Klimaszewska K, Noceda C, Pelletier G, Label P, Rodriguez R, Lelu-Walter MA (2008) Biological
characterization of young and aged embryogenic cultures of Pinus pinaster (Ait.) In Vitro Cell
Dev-Pl 45 (1): 20-33. doi 10.1007/s11627-008-9158-6.
- Hernández R, Ramírez T, Noa-Carrazana JC, Rodríguez-Fernández R, Cañal MJ, FloresEstévez N, Corujo M, Noceda C, Ventura J (2007) Generation of five new Musa hybrids with
resistance to black Sigatoka and high yield. Amer J Agric & Biol Sci 2(2): 43-48. doi
10.3844/ajabssp.2007.43.48.
- Hernández R, Rodríguez R, Ramírez T, Cañal MJ, Guillen D, Noceda C, Escalona M, Corujo M,
Ventura J (2007) Genetic and morphoagronomic characterization of plantain variants of
Musa AAB clone ´CEMSA ¾`. Journal of Food, Agriculture & Environment 5(1): 220-223.
- Aragón C, Escalona M, Capote I, Pina D, Cejas I, Rodríguez R, Noceda C, Sandoval J, Roels S,
Debergh P, González-Olmedo JL (2006) Metabolic importance of starch in the acclimation of
plantain ‘CEMSA 3/4’ (AAB) plants. INFOMUSA 15 (1,2): 32-35.
- Roels S, Noceda C, Escalona M, Sandoval J, Canal MJ, Rodriguez, Debergh P (2006) The effect
of headspace renewal in a Temporary Immersion Bioreactor on plantain (Musa AAB) shoot
proliferation and quality. Plant Cell Tiss Org Cult 84 (2): 155-163. doi 10.1007/s11240-0059013-y.
- Roels S, Escalona M, Cejas I, Noceda C, Rodriguez R, Canal MJ, Sandoval J, Debergh P (2005)
Optimization of plantain (Musa AAB) micropropagation by temporary immersion system.
Plant Cell Tiss Org Cult 82 (1): 57-66. doi 10.1007/s11240-004-6746-y.
- Noceda C, Quiala, E, Jiménez E, Rodríguez R, Cañal MJ, Gómez R, Barbón R, Capote A, Pérez
N, de Feria M, Chávez M (2002) Proporciones diferenciales de poliaminas en explantes
foliares y suspensiones celulares embriogénicas y no embriogénicas en Coffea canephora
P. var. Robusta. Café Cacao 3(2): 39-41.
- Noceda C, Sierra SG, Martínez JL (1997) Histopathology of UV-B irradiated brown trout (Salmo
trutta L.) skin. Dis Aquat Org 31: 103-10.
Reviews
- Uribe-Moraga M, Cañal MJ, Noceda C, Ríos D, Fraga MF, Ferrando A, Altabella T, Tiburcio AF,
Rodríguez R (2004) Polyamines in herbaceous and woody plants. Current Topics in Plant
Biology 5: 53-62.
Chapters in indexed books
- Nogales A, Noceda C, Ragonezzi C, Cardoso H, Campos MD, Frederico AM, Sircar D, Kumar
SR, Polidoros P, Peixe P, Arnhold-Schmitt B (in press) Functional marker development from
AOX genes requires deep phenotyping and individualized diagnosis. Sub-chapter in
Arnholdt-Schmitt B. From AOX diversity to functional marker development. Gupta KJ, Mur
LAJ, Neelwarne B (eds.) Alternative respiratory pathways in higher plants. John Wiley &
Sons, Inc. (pp. x-y) ISBN: 978-1-118-79046-5
https://sites.google.com/site/alternativerespiratorypathways/
- Noceda C, Svensson JT, Nogales A, Arnholdt-Schmitt B (in press) AOX gene diversity can affect
DNA methylation and genome organization relevant for functional marker development. Subchapter in Arnholdt-Schmitt B. From AOX diversity to functional marker development. Gupta
KJ, Mur LAJ, Neelwarne B (eds.) Alternative respiratory pathways in higher plants. John
Wiley & Sons, Inc. (pp. x-y) ISBN: 978-1-118-79046-5
https://sites.google.com/site/alternativerespiratorypathways/
Conferences with abstracts in indexed books
- Noceda C, Salaj T, Pérez M, Canal MJ, Salaj J, Rodriguez R (2007) DNA methylation status and
free polyamine content in Pinus nigra embryogenic tissues with different maturation ability.
Poster. VII International Symposium in the series “Recent Advances in Plant Biotechnology“.
Jun 10-16. Plant Biotechnology: Impact on High Quality Plant Production. Book of abstracts:
81-82 (193 pp). Stará Lesná, High Tatras (Slovakian Republic). ISBN: 978-80-89088-51-5 (In
Web of Science)
NON-INDEXED PUBLICATIONS
Chapters in books
- Rodríguez R, Fernández-Fraga M, Pacheco JC, Berdasco M, Diego LB, Noceda C, Hasbun R,
Valledor L, Hernández R, Cañal MJ (2004) Envejecimiento y cambio de fase: hacia la
propagación de genotipos élite independientes de edad. In: Sánchez-Olate, Ríos-Leal D
(eds) II Curso Internacional de Postgrado en Biotecnología Forestal. Desde el Microinjerto a
la embriogénesis somática. 2004 Mar 08-11. Univ. de Concepción. Chile. pp.: 1-17
Conference Proceedings in books
- Noceda C, Cañal MJ, Fraga MF, Escalona M, González-Olmedo J, Cejas I, Santamaría E,
Hernández R, Sandoval J, Roels S, Debergh P, Gómez-Kosky R, Berdasco M, Ramírez T,
Rodríguez R (2005) Participacion de las poliaminas en la morfogénesis vegetal. Oral
communication and Poster. Bioveg 2005 Feb 7-11. In: Cabrales Hernández G, Martínez
Montero ME, Santiesteban Toca C, Loyola Martínez O, Ortega Reyes Y, Romero Podio R
(eds) Abstracts: 114 (116 pp). Proceedings: 909-914 (926 pp). Centro de Bioplantas, Ciego
de Ávila (Cuba). ISBN: 959-16-0300-2.
- Noceda C, Rodríguez R, Hernández R, Alonso M, Escalona M, González-Olmedo J, Cejas I,
Sandoval J, Roels S, Debergh P, Ramírez T, Cañal MJ (2005) (Epi)genetic characterization
of Musa regenerants. Oral communication. Bioveg 2005 Feb 7-11. In: Cabrales Hernández
G, Martínez Montero ME, Santiesteban Toca C, Loyola Martínez O, Ortega Reyes Y,
Romero Podio R (eds) Abstracts: 4 (116 pp). Proceedings: 13-20 (926 pp). Centro de
Bioplantas, Ciego de Ávila (Cuba). ISBN: 959-16-0300-2
- Rodríguez R, Diego LB, Berdasco M, Fraga MF, Risueño C, Testillano PS, Noceda C,
Santamaría E, Hasbun R, Valledor L, Cañal MJ (2005) Impacto de la maduraciónenvejecimiento en la clonación de especies agroforestales: sistemas de multiplicación,
competencia-expresión de genes candidatos y validación de los regenerantes. Invited
plenary conference. Bioveg 2005 Feb 7-11. In: Cabrales Hernández G, Martínez Montero
ME, Santiesteban Toca C, Loyola Martínez O, Ortega Reyes Y, Romero Podio R (eds)
Abstracts: 23 (116 pp). Poceedings: 159-164 (926 pp): Centro de Bioplantas, Ciego de Ávila
(Cuba). ISBN: 959-16-0300-2.
- Cejas I, Capote I, Escalona M, Noceda C, Rodríguez R, Cañal MJ, Roels S, Sandoval J,
Debergh P (2005) Protocolo para la proliferación de plátano Musa AAB (CEMSA ¾) con el
empleo de meta-topolina en Biorreactores de Inmersión Temporal. Oral communication.
Bioveg 2005 Feb 7-11. In: Cabrales Hernández G, Martínez Montero ME, Santiesteban Toca
C, Loyola Martínez O, Ortega Reyes Y, Romero Podio R (eds) Abstracts: 3 (116 pp).
Proceedings: 7-13 (926 pp) Centro de Bioplantas, Ciego de Ávila (Cuba). ISBN: 959-160300-2.
- Rodríguez R, Fraga M, Diego L, Berdasco M, Hasbun R, Noceda C, Mendivil C, Salajová T
Radojevic L, Escalona M, Roels S, Debergh P, Cañal MJ (2003) Micropropagation and
Physiological aspects. Invited plenary conference. In: Espinel S, Barredo I, Ritter E (eds)
Sustainable Forestry, Wood Products and Biotechnology. DFA-AFA Press, Vitoria-Gasteiz,
Spain International Scientific Congress BIOFOR´02 Nov 11-14. Arabako Foru Aldundia.
Depósito legal: VI-552/03. 1, 15-30. Vitoria (España).
OTHER MEETINGS
With abstracts
- Noceda C, Flores J, Santos E, Burbano R, Peralta E, Sosa D (2014) Estrategia para el estudio
de las alteraciones moleculares que causan la variación somaclonal en banano. VI Simposio
de Mejoramiento y Conservación de Recursos Fitogenéticos. XIX Congreso Internacional del
INCA (Cuba)
- Noceda C, Porfírio S, Santos-Macedo E, Peixe A, Birgit Arnholdt-Schmitt B (2014) Papel del
estrés en el enraizamiento adventicio de olivo. IV Simposio de Ecofisiología Vegetal. XIX
Congreso Internacional del INCA (Cuba)
- Noceda C, Porfirio S, Santos-Macedo E, Peixe A, Arnhold-Schmitt A (2014) Gestión del estrés
oxidativo por la oxidasa alternativa durante la inducción del enraizamiento adventicio en
olivo. Memorias del 2º Simposio de Fisiología Vegetal. Archivos Académicos de la
Universidad San Francisco (Ecuador). 1:10. ISBN: 978-9978-68-069-8
- Noceda C (2014) Epigenetic reprogramming during the induction of somatic embryogenesis.
Cases in Pinaceae. Presentación oral. II Congreso Internacional de Biotecnología y
Biodiversidad (CIBBII). Abstracts. Guayaquil, Ecuador.
- Noceda C, Flores J, Santos E, Cevallos JM, Manzano P, Peralta EL (2014) Principales
resultados sobre la producción de material de siembra de banano y plátano: cultivo de
tejidos, saneamiento y variación somaclonal. Conferencia invitada. II Congreso Internacional
de Biotecnología y Biodiversidad (CIBBII). Abstracts. Guayaquil, Ecuador.
- Noceda C, Santos-Macedo E, Peixe A, Arnholdt-Schmitt B (2013). Increased alternative oxidase
(AOX) expression early during adventitious root induction in shoot cuttings of Olea europaea
L. Olomouc Biotech 2013. Plant Biotechnology: Green for Good II. June 17-21. Abstracts: 88.
Centre of the Region Haná for Biotechnological and Agricultural Research. Olomouc, Czech
Republic.
- Rodríguez R, Cañal MJ, Valledor L, Hasbún R, Noceda C, Santamaría E, Meijón M, Feito I,
Rodríguez JL, Testillano P, Risueño C, Berdasco M, Fraga M (2006) Physiological and
epigenetic traits during maturation of agroforesntry species. Invited conference. COST E-28
WG2. Meeting on Functional genomics of tree maturation and reproduction. Feb 2-3.
Abstracts: 17-18. Dpto. Biología Vegetal. Universidad de Alcalá. Alcalá de Henares, Madrid
(España)
- Klimaszewska K, Noceda C, Pelletier G, Label P, Rodríguez R, Lelu-Walter MA (2006) Agedependent biological characteristics of embryonal mass of maritime pine in relations to the
embryogenic potential. Poster. 2006 Meeting of the Society for In Vitro Biology. Jun 3-7.
Late abstracts: 8 (12 pp). Minneapolis (USA)
- Noceda C, Rodríguez R, Cañal MJ, Alonso M, Hernández R, Escalona M, González-Olmedo J,
Cejas I, Sandoval J, Roels S, Debergh P, Ramírez T (2004) (Epi)genetic certification of Musa
regenerants from BIT® systems. Invited conference. COST 843. 2004 Nov 18-21. Heraklio
(Greece).
- Escalona M, González-Olmedo J, Cejas I, Aragón C, Capote I, Rodríguez R2, Cañal MJ,
Sandoval J, Hernández R, Noceda C, Alonso-Guervós M, Santamaría Fernández E, Roels
S, Debergh P (2004) Scaling-up plant production by temporary immersion and quality of
regenerants. Invited conference (Co-organizator). COST 843. 2004. Abstracts: 23. Oviedo
(Spain)
- Cejas I, Escalona M, Capote I, Noceda C, Rodriguez R, Canal MJ, Roels S, Debergh P,
Sandoval (2004) Protocol for proliferation of the plantain (Musa AAB) clon 'CEMSA 3/4' in
temporary immersion bioreactor. Oral communication. XVI reunión internacional ACORBAT.
Sept 26-Oct 01. In: Orozco Romero J, Orozco-Santos M, Zapata-Altaminaro R, VizcaínoGuajardo A, Morfin-Valencia A, Hernández-Bautista JA (eds) Publicación especial. Short
reports: 185 (280 pp). Oaxaca (Mexico).
- Noceda C, Hernández R, Alonso-Guervós M, Santamaría E, Escalona M, González-Olmedo J,
Cejas I, Aragón C, Capote I, Ramírez T, Sandoval J, Roels S, Debergh P, Rodríguez R,
Cañal MJ (2004) Producción de plantas de Musa AAB clon CEMSA ¾ mediante escalado en
biorreactores de inmersión temporal. Calidad de los regenerantes. Poster (Co-organizator).
BIOTEC 2004 Jul 19-23. Abstracts: 429 (594 pp) Oviedo (Spain). ISBN: 84-609-1771-1. DL:
AS-2521/2004
- Noceda C, Cejas I, Fernández P, Ramírez T, Hernández R, Gómez Kosky R, Cañal MJ,
Escalona M, Rodríguez R (2003) Definición de marcadores moleculares y métodos para
incrementar el rendimiento de organogénesis y embriogénesis somática de Musa spp.
empleando inmersión temporal (BIT®). Oral communication. Reunión SECIVTV 2003 Sept
16-20. Abstracts: 191. Palma de Mallorca (Spain)
- Rodríguez R, Hasbún R, Cañal M, Fraga M, Noceda C, Berdasco M, Diego L (2003). Molecular
Bases of Plant Ageing and Reinvigoration in Agroforestry Species. Invited plenary
conference. International Workshop on Plant Biotechnology Bioveg 2003 Apr 14-19. In:
Santos M, Cabrales G (eds) Plant Biotechnology and Sustainable Agriculture. Abstracts: 5-6
(131 pp). Centro de Bioplantas, Ciego de Ávila (Cuba)
- Noceda C, Cejas I, Fernández P, Roels S, Bernal B, Sandoval J, Hernández R, Rodríguez R,
Escalona M, Debergh P, Cañal MJ (2003) Genetic, epigenetic and phenotypic variations
along in vitro vegetative regeneration of Musa AAB clone CEMSA ¾. Oral communication.
International Workshop on Plant Biotechnology Bioveg 2003 Apr 14-19. In: Santos M,
Cabrales G (eds) Plant Biotechnology and Sustainable Agriculture. Abstracts: 29-30 (131
pp). Centro de Bioplantas, Ciego de Ávila (Cuba)
- Noceda C, Gómez Kosky R, Rodríguez R, Reyes M, Jiménez E, Cañal MJ, Rodríguez R (2003)
Extracellular proteins serologically analogue to PE32 and polyamines as markers in somatic
embryogenesis process in Musa spp.: induction, somatic embryo formation, maturation,
germination and embryogenic competency. Oral communication and Poster. International
Workshop on Plant Biotechnology Bioveg 2003 Apr 14-19. In: Santos M, Cabrales G (eds)
Plant Biotechnology and Sustainable Agriculture. Abstracts: 12-14 (131 pp). Centro de
Bioplantas, Ciego de Ávila (Cuba)
- Noceda C, Cejas I, Fernández P, Ramírez T, Hernández R, Gómez R, Escalona M, Cañal MJ,
Rodríguez R (2003) Hacia la certificación molecular y epigenética de microplantas de Musa
spp. producidas en sistemas de inmersión temporal BIT®. Poster. V Reunión de la Sociedad
Española de cultivo in vitro de Tejidos Vegetales (SECIVTV) Jun 29-Jul 2. Abstracts: 79
(104). Pamplona (Spain)
- Rodríguez R, Esaclona M, Rodríguez M, Gómez R, Reyes M, Jiménez E, Roels S, Debergh P,
Castresana C, Lauria M, Lund G, Ritter E, Espinel S, Aragonés A, García I, Telleira A,
Harvengt L, Risueño MC, Testilano P, Tiburcio A, Altabella T, Ríos D, Sánchez M, Radojevic
L, Berdasco M, Diego LB, Hasbun R, Noceda C, Bernardo S, Valledor L, Fraga M, Cañal MJ
(2002) Gene expression and epigenetic modifications related to maturation and phasechange in Pinus radiata D. Don. Invited conference. International Scientific Congress
BIOFOR´02 Nov 11-14. Sustainable Forestry, Wood Products and Biotechnology. Vitoria
(Spain).
- Noceda C, Rodríguez M, Gómez Kosky R, Reyes M, Jiménez E, Fernández Fraga M, Cañal MJ,
Rodríguez R (2001) Participación de proteínas serológicamente análogas a PE32 en la
embriogénesis somática de Musa. Oral communication. XIV Reunión de la Sociedad
Española de Fisiología Vegetal y VII Congreso Hispano-Luso. Sep 27. In: Álvarez Tinaut
MC, Paredes Maña MA, Espinosa Borreguero F (eds) Resúmenes: 248 (367 pp). Badajoz
(Spain) ISBN: 84-7723-455-8. DL: BA-488-2001
- Noceda C, Grau R, Reyes M, y Gómez-Kosky R (1999) Influencia de diversos factores sobre la
embriogénesis somática de alta frecuencia en explantes florales masculinos de Musa cvs.
Gran Enano (AAA) y FHIA-18 (AAAB). Oral communication. V Coloquio Internacional de
Biotecnología Vegetal 1999 Jun 16-19. In: Alvarado Capó Y, Gregorio Chaviano O (eds)
Libro de Reportes Cortos. pp 125-127 (266 pp). Instituto de Biotecnología de las Plantas.
Santa Clara. (Cuba). ISBN: 959-7122-03-0
- Noceda C, Jiménez E, Barbón R, Capote A, Quiala E, Chávez M, Pérez N, Pérez JC (1998)
Estudio del potencial embriogénico en diferentes explantes foliares de Coffea canephora P.
cv. Robusta. Poster. III Latin-American Meeting on Plant Biotechnology FAO-REDBIO 1998
Jun 1-5. Abstracts. 19-20 (516 pp). Havana (Cuba)
- Sierra SG, Noceda C, Herrero P, Martínez JL (1997) Sunburn lesions in salmonid skin
experimentally irradiated with ultraviolet-B Poster. VIII international Conference of European
Association of Fish Pathologists (EAFP). Abstracts: P-023. Edinbourgh, Scotland (UK)
-Sierra SG, Noceda C, Martínez JL (1996) Irradiaciones experimentales de truchas con UV-B.
Efectos tegumentarios. Poster. I Reunión Nacional de Inmunología y Patología de Peces.
Abstracts: P1. University of Extremadura, Badajoz (Spain)
Without publication
- Noceda C (2014) Epigenética: cómo el ambiente modifica la expresión de los genes.
Conferencia invitada. Semana Verde, UEES 2014: Jornadas Ambientales. Universidad
Estatal del Espíritu Santo, Guayaquil, Ecuador.
- Noceda C (2014) Uso de tecnologías OMICAS para el desarrollo de la biotecnología microbiana
e industrial: Introducción a las tecnologías OMICAS, aplicaciones biotecnológicas para el
biodescubrimiento a partir de microorganismo y su uso en la industria. Conferencia invitada.
Taller “Bioprospección y metagenómica microbiana. Avances de los estudios moleculares en
banano”. Universidad de Las Amáricas, Quito, Ecuador.
- Noceda C (2014) Investigaciones del CIBE en Cultivo de tejidos y biología molecular. Taller
“Bioprospección y metagenómica microbiana. Avances de los estudios moleculares en
banano”. Conferencia invitada. Universidad de Las Amáricas, Quito, Ecuador.
- Noceda C (2014) Investigaciones del CIBE en Bioproductos y Fitopatología. Conferencia
invitada. Taller “Bioprospección y metagenómica microbiana. Avances de los estudios
moleculares enbanano”. Universidad de Las Amáricas, Quito, Ecuador.
- Peixe A, Noceda C, Porfírio S (2013) Procurando compreender a dificuldade de enraizamento de
algumas variedades de oliveira (Olea europaea L.). Oral communication. Workshop
“Investigação em Olivicultura e Azeite –Resultados e Aplicações”. June 27-28. Pólo da Mitra,
University of Évora, Portugal.
- Noceda C (2001) Optimization of somatic embryogenesis in Musa cultivars and involvement of
polyamines and extracellular proteins. Invited conference. 22 Nov. Lab. Of Tropical Crop
Improvement. KU Leuven.
- Noceda C (1996) Residuos sólidos urbanos. I Taller Nacional de Ecología y Medio Ambiente.
PROECO 1996 Feb 29- Mar 01. Instituto de Biotecnología de las Plantas, Santa Clara
(Cuba).
THESES/DISSERTATIONS
- Noceda C (2001) Optimization of somatic embryogenesis in Musa cultivars and involvement of
polyamines and extracellular proteins. PhD thesis. University of Oviedo, Spain. Cum laude
(highest distinction). Supervisors: Prof. María J Cañal (University of Oviedo), Dr. Rafael
Gómez-Kosky, R (Insitute of Plant Biotechnology, Central University of Las Villas, Cuba).
- Noceda C (1999) Influence of different factors on somatic embryogenesis in banana (Musa spp.).
Biochemical characterization of the process. MSc thesis. Central University of Las Villas,
Cuba. Excellent (highest distinction) Supervisors: Dr. Rafael Gómez-Kosky (Insitute of Plant
Biotechnology, Central University of Las Villas), MSc Mayra Rodríguez (National Centre of
Scientific Researches, Havana).
- Noceda C (1994) Hystopathological effects of UV-B radiation in the epidermis of trout (Salmo
trutta L) Department of Biology of Organisms and Systems, University of Oviedo, Spain. 9
credits (highest distinction). Supervisor: Prof. Juan L Martínez (Univesity of Oviedo)