1. No category

Differen0al Lyn-­‐dependence of the SHIP1-­‐deficient mast cell phenotype Suppl. Figure 2 FL2-H: ckit - PE
102
101
0
10
10
0.45
0
0.66
1
2
3
Lyn-­‐/-­‐ 1.35
102
101
0
10
10
0.27
0
0.32
1
2
3
SHIP1-­‐/-­‐ 0.78
104
99.1
103
102
101
0.087
0
10
4
10
10
10
FL1-H: IgE - FITC
10
10
0
0.076
1
2
3
102
101
0
10
80
0
0
10
40
20
1
2
3
10
10
10
FL1-H: ST2 - FITC
4
60
40
20
0
0
10
10
% of Max
80
% of Max
80
% of Max
80
% of Max
100
60
con con C
88.1
wt WT-C - ST2 88.6
1
2
3
10
10
10
FL1-H: ST2 - FITC
4
3
4
10
60
40
20
0
0
10
10
2
dko 100
20
0.086
1
10
10
10
FL1-H: IgE - FITC
SHIP1-­‐/-­‐ 100
40
0.098
0
living
dko - C
Event Count: 17352
100
60
97.1
103
10
IgE Lyn-­‐/-­‐ dko 2.73
10
4
10
10
10
FL1-H: IgE - FITC
living
SHIP ko - C
Event Count: 18348
living
Lyn ko - C
Event Count: 15439
wt con con C
88.1
Lyn-­‐/-­‐ Lyn ko-C - ST2 72.3
1
2
3
10
10
10
FL1-H: ST2 - FITC
0
0
10
4
10
con con C
88.1
SHIP1-­‐/-­‐ SHIP ko-C - ST2 90.1
1
2
3
10
10
10
FL1-H: ST2 - FITC
4
10
con con C
88.1
dko dko-C - ST2 82.8
ST2 100
80
80
60
40
20
0
0
10
60
40
20
1
2
3
10
10
10
FL2-H: CD13 - PE
4
10
con con C
88.1
wt WT-C - CD13 88.9
% of Max
100
80
% of Max
100
80
% of Max
100
% of Max
Histogram peak intensity [%] 104
98.1
103
10
4
10
10
10
FL1-H: IgE - FITC
living
WT C
Event Count: 17276
B 104
98.1
FL2-H: ckit - PE
wt 0.81
103
FL2-H: ckit - PE
Kit 104
FL2-H: ckit - PE
A 60
40
20
0
0
10
1
2
3
10
10
10
FL2-H: CD13 - PE
40
20
0
0
10
4
10
60
con con C
88.1
Lyn-­‐/-­‐ Lyn ko-C - CD13 75.5
1
2
3
10
10
10
FL2-H: CD13 - PE
0
0
10
4
10
con con C
88.1
SHIP1-­‐/-­‐ SHIP ko-C - CD13 91.9
1
2
WB: α-­‐SHIP1
50 -­‐ WB: α-­‐Lyn
85 -­‐ WB: α-­‐p85
Nunes de Miranda et al. 2016 CZ 4T_3 V355 Proliferation
Cell count [105/ml]
wt Lyn-­‐/-­‐ SHIP1-­‐/-­‐ dko 120 -­‐ D 15
wt
Lyn-/SHIP1-/dko
***
***
10
***
5
0
0
4
8
Proliferation [days]
4
10
con con C
88.1
dko dko-C - CD13 86.3
CD13 C 3
10
10
10
FL2-H: CD13 - PE
12